You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Overview

  • Product nameAnti-EPS8 antibodySee all EPS8 primary antibodies ...
  • Description
    Rabbit polyclonal to EPS8
  • Tested applicationsWB, IHC-P more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 761- 822 (DELRTVCPEG ARVYSQITVQ KAALEDSSGS SELQEIMRRR QEKISAAASD SGVESFDEGS SH) of Human EPS8 (NP_004438).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive controlWB: H1299, HeLa cell lysates IHC-P: Gastric CA

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 1% BSA, 1X PBS, pH 7
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified by antigen affinity chromatography.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab96144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/500 - 1/3000. Predicted molecular weight: 92 kDa.
IHC-P IHC-P: 1/100 - 1/250.

Target

  • FunctionUpon binding to EGF receptor enhances EGF-dependent mitogenic signals. Can bind multiple cellular targets.
  • Tissue specificityExpressed in all tissues analyzed, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Expressed in all epithelial and fibroblastic lines examined and in some, but not all, hematopoietic cells.
  • Sequence similaritiesBelongs to the EPS8 family.
    Contains 1 PH domain.
    Contains 1 SH3 domain.
  • DomainThe SH3 domain mediates interaction with SHB.
  • Post-translational
    modifications    Phosphorylated by several receptor tyrosine kinases.

    • Target information above from: UniProt accession Q12929 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        Epidermal growth factor receptor kinase substrate 8 antibodyEpidermal growth factor receptor pathway substrate 8 antibodyEPS 8 antibody
        EPS8 antibodyEPS8_HUMAN antibody
      see all

    Anti-EPS8 antibody images

    • Western blot - EPS8 antibody (ab96144)
      Western blot - EPS8 antibody (ab96144)
      All lanes : Anti-EPS8 antibody (ab96144) at 1/1000 dilution

      Lane 1 : H1299 whole cell lysate
      Lane 2 : Hela whole cell lysate

      Lysates/proteins at 30 µg per lane.


      Predicted band size : 92 kDa
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EPS8 antibody (ab96144)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EPS8 antibody (ab96144)
      ab96144, at 1/100 dilution, staining EPS8 in paraffin embedded Gastric CA by Immunohistochemical analysis.

    References for Anti-EPS8 antibody (ab96144)

    ab96144 has not yet been referenced specifically in any publications.

    Publishing research using ab96144 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab96144 Submit a Question for ab96144

    There are currently no Abreviews or Questions for ab96144.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"