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Overview

  • Product nameAnti-ERCC1 antibodySee all ERCC1 primary antibodies ...
  • Description
    Goat polyclonal to ERCC1
  • Specificityab77405 is expected to recognise both reported isoforms (NP_973730.1 and NP_001974.1).
  • Tested applicationsWB, ELISA more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide: DPGKDKEGVPQPS-C, corresponding to N terminal amino acids 2-14 of Human ERCC1 (NP_973730.1; NP_001974.1).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive controlA431 and Kelly lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab77405 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab77405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
ELISA
  • Application notesPeptide ELISA: Antibody detection limit dilution 1/64000.
    WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 32 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionStructure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair.
    • Involvement in diseaseDefects in ERCC1 are the cause of cerebro-oculo-facio-skeletal syndrome type 4 (COFS4) [MIM:610758]. COFS is a degenerative autosomal recessive disorder of prenatal onset affecting the brain, eye and spinal cord. After birth, it leads to brain atrophy, hypoplasia of the corpus callosum, hypotonia, cataracts, microcornea, optic atrophy, progressive joint contractures and growth failure. Facial dysmorphism is a constant feature. Abnormalities of the skull, eyes, limbs, heart and kidney also occur.
    • Sequence similaritiesBelongs to the ERCC1/RAD10/SWI10 family.
    • Cellular localizationNucleus.

      • Target information above from: UniProt accession P07992 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          COFS 4 antibodyCOFS4 antibodyDNA excision repair protein ERCC 1 antibody
          DNA excision repair protein ERCC-1 antibodyDNA excision repair protein ERCC1 antibodyERCC 1 antibodyERCC1 antibodyERCC1_HUMAN antibodyExcision repair cross complementing 1 antibodyExcision Repair Cross Complementing Rodent Repair Deficiency Complementation Group 1 antibodyExcision repair protein antibodyRAD 10 antibodyRAD10 antibodyUV 20 antibodyUV20 antibody
        see all

      Anti-ERCC1 antibody images

      References for Anti-ERCC1 antibody (ab77405)

      ab77405 has not yet been referenced specifically in any publications.

      Publishing research using ab77405 ? Please let us know so that we can cite the reference in this datasheet

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"