Anti-PICH antibody (ab88560)
Key features and details
- Mouse polyclonal to PICH
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PICH antibody
See all PICH primary antibodies -
Description
Mouse polyclonal to PICH -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human PICH. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (AAH08808.1)
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Positive control
- Hela cell lysate, PICH transfected 293T cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88560 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 141 kDa.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 141 kDa. |
Target
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Function
DNA helicase that acts as an essential component of the spindle assembly checkpoint. Contributes to the mitotic checkpoint by recruiting MAD2 to kinetochores and monitoring tension on centromeric chromatin. Acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase. -
Sequence similarities
Belongs to the SNF2/RAD54 helicase family.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 2 TPR repeats. -
Post-translational
modificationsPhosphorylation by PLK1 prevents the association with chromosome arms and restricts its localization to the kinetochore-centromere region. -
Cellular localization
Chromosome > centromere. Chromosome > centromere > kinetochore. Localizes to kinetochores, inner centromeres and thin threads connecting separating chromosomes even during anaphase. In prometaphase cells, it mostly concentrates in between kinetochores. In metaphase, it localizes to numerous thin threads that stretch between sister kinetochores of the aligned chromosomes and are composed of catenated centromeric DNA. Evolution from inner centromeres to thin threads takes place in response to tension. Resolution of thin threads requires topoisomerase 2-alpha (TOP2A) after anaphase onset. - Information by UniProt
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Database links
- Entrez Gene: 54821 Human
- Omim: 300687 Human
- SwissProt: Q2NKX8 Human
- Unigene: 47558 Human
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Alternative names
- ATP dependent helicase ERCC6 like antibody
- ATP-dependent helicase ERCC6-like antibody
- DNA excision repair protein ERCC 6 like antibody
see all
Images
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All lanes : Anti-PICH antibody (ab88560) at 1 µg/ml
Lane 1 : PICH transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 141 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
Anti-PICH antibody (ab88560) at 1 µg/ml + Hela cell lysate at 50 µg
Predicted band size: 141 kDa
Observed band size: 141 kDa
Additional bands at: 57 kDa. We are unsure as to the identity of these extra bands.
Datasheets and documents
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Datasheet download
References (2)
ab88560 has been referenced in 2 publications.
- Dutta A et al. PLK-1 Interacting Checkpoint Helicase, PICH, Mediates Cellular Oxidative Stress Response. Epigenomes 6:N/A (2022). PubMed: 36278682
- Panier S et al. SLX4IP Antagonizes Promiscuous BLM Activity during ALT Maintenance. Mol Cell 76:27-43.e11 (2019). PubMed: 31447390