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Western blot - ERG antibody (ab28662)
(enlarge)
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Product Name
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ERG antibody
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See all ERG antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ERG
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Immunogen
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A region within synthetic peptide: SKAAQPSPST VPKTEDQRPQ LDPYQILGPT SSRLANPGSG QIQLWQFLLE, corresponding to internal sequence amino acids 253-302 of Human ERG
BLAST 'SKAAQPSPST VPKTEDQRPQ LDPYQILGPT SSRLANPGSG QIQLWQFLLE ' with  or 
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Reacts with
(species key)
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Hu
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Specificity
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This antibody should detect both isoforms of ERG.
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/15000.
WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
Is unsuitable for IHC-P.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Jurkat cell lysate, HepG2 cell lysate.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Lysine methylation
Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Stem Cells >> Hematopoietic Progenitors >> Intracellular Molecules
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
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Relevance
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v-ets erythroblastosis virus E26 oncogene like isoform 2 (ERG) is a transcriptional regulator. It may participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Purification notes
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Purified by Protein G/A affinity chromatography.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ERG antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ERG antibody images, references, products related to ab28662 and other tools.
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ERG antibody images:
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 Western blot - ERG antibody (ab28662)
ERG antibody (ab28662) at 5 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 52 kDa
Observed band size : 52 kDa
Gel concentration: 12%
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Search PubMed (MEDLINE) for references to ERG
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ERG antibody - more information
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Customer reviews (feedback) regarding ERG antibody |
Customer FAQs regarding ERG antibody |
Protocols for ERG antibody |
Price and availability of products related to ERG antibody |
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ERG antibody - related products:
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ERG antibody - other tools:
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Contact Abcam with a Technical Enquiry about ERG antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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