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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ERG antibody (ab77258)
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Product Name
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ERG antibody
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See all ERG antibodies (3)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to ERG
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Immunogen
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Synthetic peptide: PNTRLPTSHMPSH from the C terminal region of Human ERG (100% homology for isoforms NP_891548.1 and NP_004440.1))(Peptide available as ab87722.)
BLAST 'PNTRLPTSHMPSH' with  or 
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Rat
(Due to sequence homology)
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Specificity
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Expected to recognise both reported isoforms (NP_891548.1; NP_004440.1)
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Tested applications
(see key)
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ELISA, IHC-P
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Peptide ELISA: Antibody detection limit dilution 1/16000.
IHC-P: Use at a concentration of 10 - 20 µg/ml. Steamed antigen retrieval with citrate buffer pH 6 is recommended.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human prostate tissue.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Lysine methylation
Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Stem Cells >> Hematopoietic Progenitors >> Intracellular Molecules
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
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Relevance
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v-ets erythroblastosis virus E26 oncogene like isoform 2 (ERG) is a transcriptional regulator. It may participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ERG antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ERG antibody images, references, products related to ab77258 and other tools.
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ERG antibody images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ERG antibody (ab77258)
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human prostate tissue, using ab77258 at a 10µg/ml. Note the nucleolar staining in some of the epithelial cells.
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Search PubMed (MEDLINE) for references to ERG
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ERG antibody - more information
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Customer reviews (feedback) regarding ERG antibody |
Customer FAQs regarding ERG antibody |
Protocols for ERG antibody |
Price and availability of products related to ERG antibody |
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ERG antibody - related products:
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ERG antibody - other tools:
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Contact Abcam with a Technical Enquiry about ERG antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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