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Overview
Product name
Anti-ERG antibody
See all ERG products (7) ...
Description
Rabbit polyclonal to ERG
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Chicken, Cow
Immunogen
Synthetic peptide corresponding to a region within internal sequence amino acids 360-409 (IMTKVHGKRY AYKFDFHGIA QALQPHPPES SLYKYPSDLP YMGSYHAHPQ) of Human ERG (NP_004440).
Positive control
Human fetal liver lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Lysine methylation
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Stem Cells >> Hematopoietic Progenitors >> Intracellular Molecules
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation
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Western blot - ERG antibody (ab87105)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab87105 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 52 kDa.
Target
Function
Transcriptional regulator. May participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure.
Involvement in disease
Defects in ERG are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving ERG is found in patients with Erwing sarcoma. Translocation t(21;22)(q22;q12) with EWSR1.
Note=Chromosomal aberrations involving ERG have been found in acute myeloid leukemia (AML). Translocation t(16;21)(p11;q22) with FUS. Translocation t(X;21)(q25-26;q22) with ELF4.
Sequence similarities
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Contains 1 PNT (pointed) domain.
Cellular localization
Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Target information above from: UniProt accessionP11308
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Avian erythroblastosis virus E-26 (v-ets) oncogene related antibody
- D030036I24Rik antibody
- Erg 3 antibody
see all
Database links
- Entrez Gene: 374146 Chicken
- Entrez Gene: 2078 Human
- Entrez Gene: 13876 Mouse
- Entrez Gene: 170909 Rat
- Omim: 165080 Human
- SwissProt: Q90837 Chicken
- SwissProt: P11308 Human
- SwissProt: P81270 Mouse
see all
Anti-ERG antibody images:
Western blot - ERG antibody (ab87105)
Anti-ERG antibody (ab87105) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal liver lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 52 kDa
Observed band size : 62 kDa (why is the actual band size different from the predicted?)
Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 12%
References for Anti-ERG antibody (ab87105)
ab87105 has not yet been referenced specifically in any publications.
Publishing research using ab87105? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"