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Western blot - ERG antibody (ab87105)
Western blot - ERG antibody (ab87105)
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Product Name 

ERG antibody

 

See all ERG antibodies (3)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to ERG

Immunogen 

Synthetic peptide corresponding to a region within internal sequence amino acids 360-409 (IMTKVHGKRY AYKFDFHGIA QALQPHPPES SLYKYPSDLP YMGSYHAHPQ) of Human ERG (NP_004440).

Reacts with 
(species key)

Hu, Ms, Rat

Tested applications 
(see key)

WB


Abreviews 

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(see key)


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Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 52 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human fetal liver lysate.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Lysine methylation
Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Stem Cells >> Hematopoietic Progenitors >> Intracellular Molecules
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes

Relevance 

v-ets erythroblastosis virus E26 oncogene like isoform 2 (ERG) is a transcriptional regulator. It may participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure.

 

Alternative names ERG antibody (ab87105)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

2078    (Human)
13876    (Mouse)
170909    (Rat)

Omim

    

165080    (Human)

SwissProt

    

Q8IXK9    (Human)
P11308    (Human)
P81270    (Mouse)
Q3UQJ4    (Mouse)
Q6IMZ7    (Rat)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: None
Constituents: 2% Sucrose, PBS

Form 

Lyophilised
Add 50 µl of distilled water. Final antibody concentration is 1 mg/ml.

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ERG antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for ERG antibody images, references, products related to ab87105 and other tools.

 

ERG antibody images:

Product image  Western blot - ERG antibody (ab87105)

Western blot - ERG antibody (ab87105)

ERG antibody (ab87105) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal liver lysate at 10 µg

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Predicted band size : 52 kDa
Observed band size : 62 kDa (why is the actual band size different from the predicted?)
Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.

Gel concentration: 12%

 
 

Search PubMed (MEDLINE) for references to ERG

 

ERG antibody - more information

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ERG antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Positive Controls for ERG antibody

ab29885

  

Liver (Human) Nuclear Lysate - fetal normal tissue

 

Compatible Secondaries for ERG antibody

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other ERG Antibodies

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ERG antibody - other tools:

Contact Abcam with a Technical Enquiry about ERG antibody

 

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