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Western blot - ERG antibody (ab87105)
(enlarge)
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Product Name
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ERG antibody
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See all ERG antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ERG
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Immunogen
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Synthetic peptide corresponding to a region within internal sequence amino acids 360-409 (IMTKVHGKRY AYKFDFHGIA QALQPHPPES SLYKYPSDLP YMGSYHAHPQ) of Human ERG (NP_004440).
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 52 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human fetal liver lysate.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Lysine methylation
Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Stem Cells >> Hematopoietic Progenitors >> Intracellular Molecules
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
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Relevance
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v-ets erythroblastosis virus E26 oncogene like isoform 2 (ERG) is a transcriptional regulator. It may participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Lyophilised
Add 50 µl of distilled water. Final antibody concentration is 1 mg/ml.
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ERG antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ERG antibody images, references, products related to ab87105 and other tools.
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ERG antibody images:
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 Western blot - ERG antibody (ab87105)
ERG antibody (ab87105) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal liver lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 52 kDa
Observed band size : 62 kDa (why is the actual band size different from the predicted?)
Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 12%
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Search PubMed (MEDLINE) for references to ERG
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ERG antibody - more information
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Customer reviews (feedback) regarding ERG antibody |
Customer FAQs regarding ERG antibody |
Protocols for ERG antibody |
Price and availability of products related to ERG antibody |
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ERG antibody - related products:
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ERG antibody - other tools:
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Contact Abcam with a Technical Enquiry about ERG antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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