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Anti-ERK1 + ERK2 antibody (ab17942)

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Overview

Product name

Anti-ERK1 + ERK2 antibody
See all ERK1 + ERK2 products (22) ...

Description

Rabbit polyclonal to ERK1 + ERK2

Tested applications

IHC-P, ICC, ICC/IF, IP, IHC-Fr, WBmore details

Cross reactivity

Reacts with

Mouse, Rat, Cow, Dog, Human

Immunogen

Synthetic peptide, corresponding to C terminal amino acids 317-339 of Human ERK1 + ERK2.

Positive control

A431, PC12, and NIH3T3 cells.

General notes

Please note that this is an intracellular epitope.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 1mg/ml BSA. pH 7.3

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

  • Western blot - ERK1 + ERK2 antibody (ab17942)Western blot - ERK1 + ERK2 antibody (ab17942) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ERK1 + ERK2 antibody (ab17942)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ERK1 + ERK2 antibody (ab17942) image (enlarge)

  • Western blot - ERK1 + ERK2 antibody (ab17942)Western blot - ERK1 + ERK2 antibody (ab17942) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab17942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational
modifications

Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.

Cellular localization

Nucleus.

Target information above from: UniProt accessionP28482 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • ERK 1 antibody
  • ERK 2 antibody
  • ERK-2 antibody
  • ERK1 antibody
  • ERK2 antibody
  • ERT1 antibody
  • ERT2 antibody
  • Extracellular signal regulated kinase 1 antibody
  • Extracellular signal regulated kinase 1 antibody
  • Extracellular signal regulated kinase 2 antibody
  • Extracellular signal regulated kinase 2 antibody
  • Extracellular signal-regulated kinase 2 antibody
  • HS44KDAP antibody
  • HUMKER1A antibody
  • Insulin stimulated MAP2 kinase antibody
  • MAP kinase 1 antibody
  • MAP kinase 2 antibody
  • MAP kinase isoform p42 antibody
  • MAP kinase isoform p44 antibody
  • MAPK 1 antibody
  • MAPK 2 antibody
  • MAPK 3 antibody
  • MAPK 3 antibody
  • MAPK1 antibody
  • MAPK2 antibody
  • MAPK3 antibody
  • MGC20180 antibody
  • Microtubule associated protein 2 kinase antibody
  • Mitogen activated protein kinase 1 antibody
  • Mitogen activated protein kinase 1 antibody
  • Mitogen activated protein kinase 2 antibody
  • Mitogen activated protein kinase 3 antibody
  • Mitogen activated protein kinase 3 antibody
  • Mitogen-activated protein kinase 1 antibody
  • Mitogen-activated protein kinase 2 antibody
  • MK01_HUMAN antibody
  • p38 antibody
  • p40 antibody
  • p41 antibody
  • p41mapk antibody
  • p42 MAPK antibody
  • p42-MAPK antibody
  • p42MAPK antibody
  • p42MAPK antibody
  • p44 ERK1 antibody
  • p44 MAPK antibody
  • p44ERK1 antibody
  • p44ERK1 antibody
  • p44MAPK antibody
  • p44MAPK antibody
  • PRKM 1 antibody
  • PRKM 1 antibody
  • PRKM 2 antibody
  • PRKM 2 antibody
  • PRKM 3 antibody
  • PRKM 3 antibody
  • PRKM1 antibody
  • PRKM2 antibody
  • PRKM3 antibody
  • Protein kinase mitogen activated 1 antibody
  • Protein kinase mitogen activated 1 antibody
  • Protein kinase mitogen activated 2 antibody
  • Protein kinase mitogen activated 2 antibody
  • Protein kinase mitogen activated 3 antibody
  • Protein kinase mitogen activated 3 antibody
  • Protein tyrosine kinase ERK 2 antibody
  • Protein tyrosine kinase ERK 2 antibody
see all

Anti-ERK1 + ERK2 antibody images:

  Western blot - ERK1 + ERK2 antibody (ab17942)

Western blot - ERK1 + ERK2 antibody (ab17942)



Predicted band size : 42-44 kDa


Extracts prepared from PC12 cells not stimulated (-), or stimulated with NGF (+) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to nitrocellulose. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4C, then were incubated with ERK1&2 pan antibody for two hours at room temperature in a 3% BSA-TBST buffer. After washing, membranes were incubated with goat anti-rabbit IgG alkaline phosphatase. These data show that ab17942 ERK1&2 antibody allows the total amount of ERK1&2 to be measured.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ERK1 + ERK2 antibody (ab17942)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ERK1 + ERK2 antibody (ab17942)

ab17942 at 1/200 staining human, rat and mouse kidney sections by IHC-P. The tissue sections were formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

This image is courtesy of an anonymous Abreview

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  Western blot - ERK1 + ERK2 antibody (ab17942)

Western blot - ERK1 + ERK2 antibody (ab17942)

All lanes : Anti-ERK1 + ERK2 antibody (ab17942) at 1/1000 dilution

Lane 1 : Rat spinal cord tissue homogenate from animals that underwent Sham surgery
Lane 2 : Rat spinal cord tissue homogenate from animals that underwent L5 nerve transection
Lane 3 : Rat spinal cord tissue homogenate from animals that underwent L5 nerve transection

Lysates/proteins at 20 µg per lane.

Secondary
HRP conjugated goat anti-rabbit antibody at 1/3000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 42-44 kDa
Observed band size : 42,44 kDa (why is the actual band size different from the predicted?)


Exposure time : 5 minutes

The tissue was harvested seven days post surgery, sonicated with RIPA buffer and the protein estimate made by Lowry. A 10% SDS-PAGE gel was run for 1.5 hr at 100V and transfered to PVDF membrane for 1.5 hr at 274 mA. The blot was blocked with 5% BSA for 1 hour at 23°C. The primary antibody was incubated with the blot for 18 hours at 4°C.

This image is courtesy of an anonymous Abreview

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  Immunocytochemistry/ Immunofluorescence - ERK1 + ERK2 antibody (ab17942)

Immunocytochemistry/ Immunofluorescence - ERK1 + ERK2 antibody (ab17942)

ab17942 staining ERK1 + ERK2 in human HeLa cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in formaldehyde, permeabilised in 0.025% Triton X, PBS and then blocked using 5% serum for 1 hour at 23°C. Samples were then incubated with primary antibody at 1/200 for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 568 (red) used at a 1/1000 dilution.

Image courtesy of an anonymous Abreview.

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  Immunohistochemistry (Frozen sections) - ERK1 + ERK2 antibody (ab17942)

Immunohistochemistry (Frozen sections) - ERK1 + ERK2 antibody (ab17942)

ab17942 staining ERK1 + ERK2 in Embryonic Stem Cell-induced Mouse Neurospheres by Immunohistochemistry (Frozen sections). Sections were formaldehyde-fixed prior to blocking in 10% serum for 30 minutes at room temperature. The primary antibody was diluted 1/1000 and incubated with the sample for 12 hours at 4°C. An Alexa Fluor® 594-conjugated Goat anti-Rabbit polyclonal antibody, diluted 1/1500 was used as the secondary.

This image is courtesy of an anonymous Abreview.

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  Immunohistochemistry (Frozen sections) - ERK1 + ERK2 antibody (ab17942)

Immunohistochemistry (Frozen sections) - ERK1 + ERK2 antibody (ab17942)

ab17942 staining ERK1 + ERK2 in human stomach tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with acetone and then blocked with 5% serum for 1 hour at 23°C followed by incubation with the primary antibody at a 1/200 dilution for 1 hour at 23°C. An undiluted HRP-conjugated goat anti-rabbit polyclonal was used as secondary antibody.

Image courtesy of an anonymous Abreview.

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  Immunocytochemistry - ERK1 + ERK2 antibody (ab17942)

Immunocytochemistry - ERK1 + ERK2 antibody (ab17942)

ab17942 staining ERK1 + ERK2 in AGS human gastric carcinoma cells by Immunocytochemistry. The cells were fixed in formaldehyde, permeabilised in 0.025% Triton X/ PBS and then blocked using 5% serum for 1 hour at 23°C. Samples were then incubated with the primary antibody at 1/200 for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to HRP, used undiluted.

Image courtesy of an anonymous Abreview.

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References for Anti-ERK1 + ERK2 antibody (ab17942)

This product has been referenced in:

  • Tummala H  et al. The D153del Mutation in GNB3 Gene Causes Tissue Specific Signalling Patterns and an Abnormal Renal Morphology in Rge Chickens. PLoS One 6:e21156 (2011). WB; Chicken.Read more (PubMed: 21887213) »
  • Beljanski V  et al. A novel sphingosine kinase inhibitor induces autophagy in tumor cells. J Pharmacol Exp Ther 333:454-64 (2010).Read more (PubMed: 20179157) »

See all 5 publications for this product

Publishing research using ab17942? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"