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Overview

  • Product nameAnti-ERK1 + ERK2 antibodySee all ERK1 + ERK2 primary antibodies ...
  • Description
    Rabbit polyclonal to ERK1 + ERK2
  • Tested applicationsIHC-P, ICC, ICC/IF, IP, IHC-Fr, WB more details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Dog, Human
  • Immunogen

    Synthetic peptide, corresponding to C terminal amino acids 317-339 of Human ERK1 + ERK2.

  • Positive controlA431, PC12, and NIH3T3 cells.
  • General notesPlease note that this is an intracellular epitope.

Properties

Applications

Our Abpromise guarantee covers the use of ab17942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P IHC-P: 1/200.
ICC ICC: Use at an assay dependent concentration.
ICC/IF ICC/IF: Use at an assay dependent concentration.
IP IP: Use at 1 µg/mg of lysate.
IHC-Fr IHC-Fr: 1/200.
WB WB: 1/1000. Predicted molecular weight: 42-44 kDa.

Target

  • Alternative names
      ERK 1 antibodyERK 2 antibodyERK-2 antibody
      ERK1 antibodyERK2 antibodyERT1 antibodyERT2 antibodyExtracellular signal regulated kinase 1 antibodyExtracellular signal regulated kinase 1 antibodyExtracellular signal regulated kinase 2 antibodyExtracellular signal regulated kinase 2 antibodyExtracellular signal-regulated kinase 2 antibodyHS44KDAP antibodyHUMKER1A antibodyInsulin stimulated MAP2 kinase antibodyMAP kinase 1 antibodyMAP kinase 2 antibodyMAP kinase isoform p42 antibodyMAP kinase isoform p44 antibodyMAPK 1 antibodyMAPK 2 antibodyMAPK 3 antibodyMAPK 3 antibodyMAPK1 antibodyMAPK2 antibodyMAPK3 antibodyMGC20180 antibodyMicrotubule associated protein 2 kinase antibodyMitogen activated protein kinase 1 antibodyMitogen activated protein kinase 1 antibodyMitogen activated protein kinase 2 antibodyMitogen activated protein kinase 3 antibodyMitogen activated protein kinase 3 antibodyMitogen-activated protein kinase 1 antibodyMitogen-activated protein kinase 2 antibodyMK01_HUMAN antibodyp38 antibodyp40 antibodyp41 antibodyp41mapk antibodyp42 MAPK antibodyp42-MAPK antibodyp42MAPK antibodyp42MAPK antibodyp44 ERK1 antibodyp44 MAPK antibodyp44ERK1 antibodyp44ERK1 antibodyp44MAPK antibodyp44MAPK antibodyPRKM 1 antibodyPRKM 1 antibodyPRKM 2 antibodyPRKM 2 antibodyPRKM 3 antibodyPRKM 3 antibodyPRKM1 antibodyPRKM2 antibodyPRKM3 antibodyProtein kinase mitogen activated 1 antibodyProtein kinase mitogen activated 1 antibodyProtein kinase mitogen activated 2 antibodyProtein kinase mitogen activated 2 antibodyProtein kinase mitogen activated 3 antibodyProtein kinase mitogen activated 3 antibodyProtein tyrosine kinase ERK 2 antibodyProtein tyrosine kinase ERK 2 antibody
    see all

Anti-ERK1 + ERK2 antibody images



  • Predicted band size : 42-44 kDa


    Extracts prepared from PC12 cells not stimulated (-), or stimulated with NGF (+) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to nitrocellulose. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4C, then were incubated with ERK1&2 pan antibody for two hours at room temperature in a 3% BSA-TBST buffer. After washing, membranes were incubated with goat anti-rabbit IgG alkaline phosphatase. These data show that ab17942 ERK1&2 antibody allows the total amount of ERK1&2 to be measured.
  • ab17942 at 1/200 staining human, rat and mouse kidney sections by IHC-P. The tissue sections were formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

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  • All lanes : Anti-ERK1 + ERK2 antibody (ab17942) at 1/1000 dilution

    Lane 1 : Rat spinal cord tissue homogenate from animals that underwent Sham surgery
    Lane 2 : Rat spinal cord tissue homogenate from animals that underwent L5 nerve transection
    Lane 3 : Rat spinal cord tissue homogenate from animals that underwent L5 nerve transection

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP conjugated goat anti-rabbit antibody at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42-44 kDa
    Observed band size : 42,44 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

    This image is courtesy of an anonymous Abreview

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  • ab17942 staining ERK1 + ERK2 in human HeLa cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in formaldehyde, permeabilised in 0.025% Triton X, PBS and then blocked using 5% serum for 1 hour at 23°C. Samples were then incubated with primary antibody at 1/200 for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 568 (red) used at a 1/1000 dilution.

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  • ab17942 staining ERK1 + ERK2 in Embryonic Stem Cell-induced Mouse Neurospheres by Immunohistochemistry (Frozen sections). Sections were formaldehyde-fixed prior to blocking in 10% serum for 30 minutes at room temperature. The primary antibody was diluted 1/1000 and incubated with the sample for 12 hours at 4°C. An Alexa Fluor® 594-conjugated Goat anti-Rabbit polyclonal antibody, diluted 1/1500 was used as the secondary.

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  • ab17942 staining ERK1 + ERK2 in human stomach tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with acetone and then blocked with 5% serum for 1 hour at 23°C followed by incubation with the primary antibody at a 1/200 dilution for 1 hour at 23°C. An undiluted HRP-conjugated goat anti-rabbit polyclonal was used as secondary antibody.

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  • ab17942 staining ERK1 + ERK2 in AGS human gastric carcinoma cells by Immunocytochemistry. The cells were fixed in formaldehyde, permeabilised in 0.025% Triton X/ PBS and then blocked using 5% serum for 1 hour at 23°C. Samples were then incubated with the primary antibody at 1/200 for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to HRP, used undiluted.

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References for Anti-ERK1 + ERK2 antibody (ab17942)

This product has been referenced in:
  • Buggele WA  et al. Influenza A Virus Infection of Human Respiratory Cells Induces Primary MicroRNA Expression. J Biol Chem 287:31027-40 (2012). WB . Read more (PubMed: 22822053) »
  • Tummala H  et al. The D153del Mutation in GNB3 Gene Causes Tissue Specific Signalling Patterns and an Abnormal Renal Morphology in Rge Chickens. PLoS One 6:e21156 (2011). WB ; Chicken . Read more (PubMed: 21887213) »

See all 6 Publications for this product

Product Wall

Displaying 1 - 10 of 22 results for Abreviews and Q&A

Application Western blot
Sample Human Cell lysate - whole cell (stomach cancer)
Loading amount 30 µg
Specification stomach cancer
Treatment 10uM WIN 55,212-2 for 24hrs
Gel Running Conditions Reduced Denaturing (8% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Apr 03 2013

Application Western blot
Sample Human Cell lysate - whole cell (U2-OS)
Loading amount 25 µg
Specification U2-OS
Gel Running Conditions Reduced Denaturing (15%)
Blocking step Odyssey buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
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Verified customer

Submitted Oct 18 2012

Gracias por contactarnos.

La concentración de este anticuerpo es dependiente del lote. He conseguido localizar el único pedido que tenemos de la Univ. de XXXXX de este producto para comprobar el lote enviado, que si no me equivoco ...

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For the detection of total ERK1+2 we recommend ab17942, a Rabbit polyclonal directed against amino acids 317-339 of Human ERK1+ERK2. This region is not subject to phosphorylation. ab17942 is covered by our Abpromise guarantee to work in ICC/IF, IHC-Fr, IH...

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Thank you for contacting us. The concentration of antibody ab17942 in lot GR46650-5 is 0.5 mg/mL. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Application Western blot
Sample Mouse Tissue lysate - whole (cortex)
Loading amount 25 µg
Specification cortex
Gel Running Conditions Reduced Denaturing (14% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted May 31 2011

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (NIH3T3 Mouse Fibroblasts)
Specification NIH3T3 Mouse Fibroblasts
Fixative Formaldehyde
Permeabilization Yes - 0.025% Triton-X in TBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Verified customer

Submitted Jan 21 2011

Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse Intestine)
Loading amount 20 µg
Specification Mouse Intestine
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Verified customer

Submitted Dec 24 2010

Application Immunohistochemistry (Frozen sections)
Sample Mouse Cell (Embryonic stem cells-induced neurosphere)
Specification Embryonic stem cells-induced neurosphere
Fixative Formaldehyde
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
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Verified customer

Submitted Nov 23 2010

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa cells)
Specification HeLa cells
Fixative Formaldehyde
Permeabilization Yes - 0.025% Triton-X in PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Verified customer

Submitted Oct 28 2010



Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"