Anti-ERK1 + ERK2 antibody (ab17942)

Overview

• Product nameAnti-ERK1 + ERK2 antibodySee all ERK1 + ERK2 primary antibodies ...
• Description
  Rabbit polyclonal to ERK1 + ERK2
• Tested applicationsIHC-P, ICC, ICC/IF, IP, IHC-Fr, WB more details
• Species reactivity
  Reacts with: Mouse, Rat, Cow, Dog, Human
  
• Immunogen

  Synthetic peptide, corresponding to C terminal amino acids 317-339 of Human ERK1 + ERK2.

• Positive controlA431, PC12, and NIH3T3 cells.
• General notesPlease note that this is an intracellular epitope.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.05% Sodium Azide
  Constituents: 50% Glycerol, PBS, 1mg/ml BSA. pH 7.3
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Signaling Pathways
  • TGF beta
  • Cytoplasmic

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • MAPK Pathway

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Alzheimer's disease
  • Tangles & Tau

Applications

Target

• FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
  Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
• Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
  Contains 1 protein kinase domain.
• DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
• Post-translational
  modificationsDually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
• Cellular localizationNucleus.

Target information above from: UniProt accession P28482 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 327672 Cow
  • Entrez Gene: 5595 Human
  • Entrez Gene: 5594 Human
  • Entrez Gene: 26417 Mouse
  • Entrez Gene: 26413 Mouse
  • Entrez Gene: 50689 Rat
  • Entrez Gene: 116590 Rat
  • Omim: 601795 Human

  • Omim: 176948 Human
  • SwissProt: P46196 Cow
  • SwissProt: P27361 Human
  • SwissProt: P28482 Human
  • SwissProt: Q63844 Mouse
  • SwissProt: P63085 Mouse
  • SwissProt: P21708 Rat
  • SwissProt: P63086 Rat
  • Unigene: 861 Human
  • Unigene: 431850 Human
  • Unigene: 196581 Mouse
  • Unigene: 8385 Mouse
  • Unigene: 2592 Rat
  • Unigene: 34914 Rat

  see all

• Alternative names
  ERK 1 antibodyERK 2 antibodyERK-2 antibody

  ERK1 antibodyERK2 antibodyERT1 antibodyERT2 antibodyExtracellular signal regulated kinase 1 antibodyExtracellular signal regulated kinase 1 antibodyExtracellular signal regulated kinase 2 antibodyExtracellular signal regulated kinase 2 antibodyExtracellular signal-regulated kinase 2 antibodyHS44KDAP antibodyHUMKER1A antibodyInsulin stimulated MAP2 kinase antibodyMAP kinase 1 antibodyMAP kinase 2 antibodyMAP kinase isoform p42 antibodyMAP kinase isoform p44 antibodyMAPK 1 antibodyMAPK 2 antibodyMAPK 3 antibodyMAPK 3 antibodyMAPK1 antibodyMAPK2 antibodyMAPK3 antibodyMGC20180 antibodyMicrotubule associated protein 2 kinase antibodyMitogen activated protein kinase 1 antibodyMitogen activated protein kinase 1 antibodyMitogen activated protein kinase 2 antibodyMitogen activated protein kinase 3 antibodyMitogen activated protein kinase 3 antibodyMitogen-activated protein kinase 1 antibodyMitogen-activated protein kinase 2 antibodyMK01_HUMAN antibodyp38 antibodyp40 antibodyp41 antibodyp41mapk antibodyp42 MAPK antibodyp42-MAPK antibodyp42MAPK antibodyp42MAPK antibodyp44 ERK1 antibodyp44 MAPK antibodyp44ERK1 antibodyp44ERK1 antibodyp44MAPK antibodyp44MAPK antibodyPRKM 1 antibodyPRKM 1 antibodyPRKM 2 antibodyPRKM 2 antibodyPRKM 3 antibodyPRKM 3 antibodyPRKM1 antibodyPRKM2 antibodyPRKM3 antibodyProtein kinase mitogen activated 1 antibodyProtein kinase mitogen activated 1 antibodyProtein kinase mitogen activated 2 antibodyProtein kinase mitogen activated 2 antibodyProtein kinase mitogen activated 3 antibodyProtein kinase mitogen activated 3 antibodyProtein tyrosine kinase ERK 2 antibodyProtein tyrosine kinase ERK 2 antibody

  see all

Anti-ERK1 + ERK2 antibody images

• 

  Western blot - ERK1 + ERK2 antibody (ab17942)
  
  
  Predicted band size : 42-44 kDa
  
  
  Extracts prepared from PC12 cells not stimulated (-), or stimulated with NGF (+) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to nitrocellulose. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4C, then were incubated with ERK1&2 pan antibody for two hours at room temperature in a 3% BSA-TBST buffer. After washing, membranes were incubated with goat anti-rabbit IgG alkaline phosphatase. These data show that ab17942 ERK1&2 antibody allows the total amount of ERK1&2 to be measured.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ERK1 + ERK2 antibody (ab17942)This image is courtesy of an anonymous Abreview
  ab17942 at 1/200 staining human, rat and mouse kidney sections by IHC-P. The tissue sections were formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

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• 

  Western blot - ERK1 + ERK2 antibody (ab17942)
  All lanes : Anti-ERK1 + ERK2 antibody (ab17942) at 1/1000 dilution
  
  Lane 1 : Rat spinal cord tissue homogenate from animals that underwent Sham surgery
  Lane 2 : Rat spinal cord tissue homogenate from animals that underwent L5 nerve transection
  Lane 3 : Rat spinal cord tissue homogenate from animals that underwent L5 nerve transection
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  HRP conjugated goat anti-rabbit antibody at 1/3000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 42-44 kDa
  Observed band size : 42,44 kDa (why is the actual band size different from the predicted?)
  
  
  Exposure time : 5 minutes

  This image is courtesy of an anonymous Abreview

  See Abreview

• 

  Immunocytochemistry/ Immunofluorescence - ERK1 + ERK2 antibody (ab17942)Image courtesy of an anonymous Abreview.
  ab17942 staining ERK1 + ERK2 in human HeLa cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in formaldehyde, permeabilised in 0.025% Triton X, PBS and then blocked using 5% serum for 1 hour at 23°C. Samples were then incubated with primary antibody at 1/200 for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 568 (red) used at a 1/1000 dilution.

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• 

  Immunohistochemistry (Frozen sections) - ERK1 + ERK2 antibody (ab17942)This image is courtesy of an anonymous Abreview.
  ab17942 staining ERK1 + ERK2 in Embryonic Stem Cell-induced Mouse Neurospheres by Immunohistochemistry (Frozen sections). Sections were formaldehyde-fixed prior to blocking in 10% serum for 30 minutes at room temperature. The primary antibody was diluted 1/1000 and incubated with the sample for 12 hours at 4°C. An Alexa Fluor® 594-conjugated Goat anti-Rabbit polyclonal antibody, diluted 1/1500 was used as the secondary.

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• 

  Immunohistochemistry (Frozen sections) - ERK1 + ERK2 antibody (ab17942)Image courtesy of an anonymous Abreview.
  ab17942 staining ERK1 + ERK2 in human stomach tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with acetone and then blocked with 5% serum for 1 hour at 23°C followed by incubation with the primary antibody at a 1/200 dilution for 1 hour at 23°C. An undiluted HRP-conjugated goat anti-rabbit polyclonal was used as secondary antibody.

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• 

  Immunocytochemistry - ERK1 + ERK2 antibody (ab17942)Image courtesy of an anonymous Abreview.
  ab17942 staining ERK1 + ERK2 in AGS human gastric carcinoma cells by Immunocytochemistry. The cells were fixed in formaldehyde, permeabilised in 0.025% Triton X/ PBS and then blocked using 5% serum for 1 hour at 23°C. Samples were then incubated with the primary antibody at 1/200 for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to HRP, used undiluted.

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