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Product name
Anti-ERK1 + ERK2 antibody
See all ERK1 + ERK2 products (22) ...
Description
Rabbit polyclonal to ERK1 + ERK2
Tested applications
IHC-P, ICC, ICC/IF, IP, IHC-Fr, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Cow, Dog, Human
Immunogen
Synthetic peptide, corresponding to C terminal amino acids 317-339 of Human ERK1 + ERK2.
Positive control
A431, PC12, and NIH3T3 cells.
General notes
Please note that this is an intracellular epitope.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 1mg/ml BSA. pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Signaling Pathways >> TGF beta >> Cytoplasmic
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> MAPK Pathway
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Tangles & Tau
Applications
Show applications key
Our Abpromise guarantee covers the use of ab17942 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/200.
ICC: Use at an assay dependent concentration.
ICC/IF: Use at an assay dependent concentration.
IP: Use at 1 µg/mg of lysate.
IHC-Fr: 1/200.
WB: 1/1000. Predicted molecular weight: 42-44 kDa.
Target
Function
Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.
Domain
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Post-translational
modifications
Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP28482
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ERK 1 antibody
- ERK 2 antibody
- ERK-2 antibody
see all
Database links
- Entrez Gene: 327672 Cow
- Entrez Gene: 5595 Human
- Entrez Gene: 5594 Human
- Entrez Gene: 26417 Mouse
- Entrez Gene: 26413 Mouse
- Entrez Gene: 50689 Rat
- Entrez Gene: 116590 Rat
- Omim: 601795 Human
see all
Anti-ERK1 + ERK2 antibody images:
Western blot - ERK1 + ERK2 antibody (ab17942)
Predicted band size : 42-44 kDa
Extracts prepared from PC12 cells not stimulated (-), or stimulated with NGF (+) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to nitrocellulose. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4C, then were incubated with ERK1&2 pan antibody for two hours at room temperature in a 3% BSA-TBST buffer. After washing, membranes were incubated
with goat anti-rabbit IgG alkaline phosphatase.
These data show that ab17942 ERK1&2 antibody allows the total amount of ERK1&2 to be measured.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ERK1 + ERK2 antibody (ab17942)
ab17942 at 1/200 staining human, rat and mouse kidney sections by IHC-P. The tissue sections were formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
This image is courtesy of an anonymous Abreview
Western blot - ERK1 + ERK2 antibody (ab17942)
All lanes : Anti-ERK1 + ERK2 antibody (ab17942) at 1/1000 dilution
Lane 1 : Rat spinal cord tissue homogenate from animals that underwent Sham surgery
Lane 2 : Rat spinal cord tissue homogenate from animals that underwent L5 nerve transection
Lane 3 : Rat spinal cord tissue homogenate from animals that underwent L5 nerve transection
Lysates/proteins at 20 µg per lane.
Secondary
HRP conjugated goat anti-rabbit antibody at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 42-44 kDa
Observed band size : 42,44 kDa (why is the actual band size different from the predicted?)
Exposure time : 5 minutes
The tissue was harvested seven days post surgery, sonicated with RIPA buffer and the protein estimate made by Lowry. A 10% SDS-PAGE gel was run for 1.5 hr at 100V and transfered to PVDF membrane for 1.5 hr at 274 mA. The blot was blocked with 5% BSA for 1 hour at 23°C. The primary antibody was incubated with the blot for 18 hours at 4°C.
This image is courtesy of an anonymous Abreview
Immunocytochemistry/ Immunofluorescence - ERK1 + ERK2 antibody (ab17942)
ab17942 staining ERK1 + ERK2 in human HeLa cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in formaldehyde, permeabilised in 0.025% Triton X, PBS and then blocked using 5% serum for 1 hour at 23°C. Samples were then incubated with primary antibody at 1/200 for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 568 (red) used at a 1/1000 dilution.
Image courtesy of an anonymous Abreview.
Immunohistochemistry (Frozen sections) - ERK1 + ERK2 antibody (ab17942)
ab17942 staining ERK1 + ERK2 in Embryonic Stem Cell-induced Mouse Neurospheres by Immunohistochemistry (Frozen sections). Sections were formaldehyde-fixed prior to blocking in 10% serum for 30 minutes at room temperature. The primary antibody was diluted 1/1000 and incubated with the sample for 12 hours at 4°C. An Alexa Fluor® 594-conjugated Goat anti-Rabbit polyclonal antibody, diluted 1/1500 was used as the secondary.
This image is courtesy of an anonymous Abreview.
Immunohistochemistry (Frozen sections) - ERK1 + ERK2 antibody (ab17942)
ab17942 staining ERK1 + ERK2 in human stomach tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with acetone and then blocked with 5% serum for 1 hour at 23°C followed by incubation with the primary antibody at a 1/200 dilution for 1 hour at 23°C. An undiluted HRP-conjugated goat anti-rabbit polyclonal was used as secondary antibody.
Image courtesy of an anonymous Abreview.
Immunocytochemistry - ERK1 + ERK2 antibody (ab17942)
ab17942 staining ERK1 + ERK2 in AGS human gastric carcinoma cells by Immunocytochemistry. The cells were fixed in formaldehyde, permeabilised in 0.025% Triton X/ PBS and then blocked using 5% serum for 1 hour at 23°C. Samples were then incubated with the primary antibody at 1/200 for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to HRP, used undiluted.
Image courtesy of an anonymous Abreview.
References for Anti-ERK1 + ERK2 antibody (ab17942)
This product has been referenced in:
- Tummala H et al. The D153del Mutation in GNB3 Gene Causes Tissue Specific Signalling Patterns and an Abnormal Renal Morphology in Rge Chickens. PLoS One 6:e21156 (2011). WB; Chicken.Read more (PubMed: 21887213) »
- Beljanski V et al. A novel sphingosine kinase inhibitor induces autophagy in tumor cells. J Pharmacol Exp Ther 333:454-64 (2010).Read more (PubMed: 20179157) »
See all 5 publications for this product
Publishing research using ab17942? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"