Anti-ERK1 + ERK2 (phospho T185 + Y187 + T202 + Y204) antibody [MAPK-YT] (ab50011)

Overview

• Product nameAnti-ERK1 + ERK2 (phospho T185 + Y187 + T202 + Y204) antibody [MAPK-YT]See all ERK1 + ERK2 primary antibodies ...
• Description
  Mouse monoclonal [MAPK-YT] to ERK1 + ERK2 (phospho T185 + Y187 + T202 + Y204)
• Specificityab50011 is specific for the active, dually-phosphorylated form of MAP kinase (ERK-1 and ERK-2, 44 kDa and 42 kDa, respectively). It does not recognise the non-phosphorylated or the monophosphorylated forms of the MAP kinase molecule or the diphosphorylated forms of JUN kinase and p38 MAP kinase.
• Tested applicationsICC/IF, ELISA, WB, IP, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Rat, Cow, Human, Saccharomyces cerevisiae, Fruit fly (Drosophila melanogaster)
  Predicted to work with: Xenopus laevis, Zebrafish
• Immunogen

  Synthetic peptide HTGFLpTEpYVAT corresponding to the phosphorylated form of human ERK1 and ERK2 activation loop.

  Run BLAST with Run BLAST with
• EpitopeThe epitope recognized by ab50011 is located in the region of phosphorylated threonine and tyrosine residues within the regulatory site of active MAP kinase (Thr185 and Tyr187 in ERK-2).
• Positive controlRat brain extract, Drosophila wing imaginal disc.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 15mM Sodium Azide
  Constituents: Ascites
• Concentration information loading...
• PurityAscites
• Clonality Monoclonal
• Clone numberMAPK-YT
• IsotypeIgG1
• Research Areas
  • Stem Cells
  • Signaling Pathways
  • TGF beta
  • Cytoplasmic

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • MAPK Pathway

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Alzheimer's disease
  • Tangles & Tau

Applications

Target

• FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
  Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
• Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
  Contains 1 protein kinase domain.
• DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
• Post-translational
  modificationsDually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
• Cellular localizationNucleus.

Target information above from: UniProt accession P28482 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 327672 Cow
  • Entrez Gene: 5595 Human
  • Entrez Gene: 5594 Human
  • Entrez Gene: 26417 Mouse
  • Entrez Gene: 26413 Mouse
  • Entrez Gene: 50689 Rat
  • Entrez Gene: 116590 Rat
  • Entrez Gene: 398985 Xenopus laevis

  • Entrez Gene: 399480 Zebrafish
  • Omim: 601795 Human
  • Omim: 176948 Human
  • SwissProt: P46196 Cow
  • SwissProt: P27361 Human
  • SwissProt: P28482 Human
  • SwissProt: Q63844 Mouse
  • SwissProt: P63085 Mouse
  • SwissProt: P21708 Rat
  • SwissProt: P63086 Rat
  • SwissProt: P26696 Xenopus laevis
  • SwissProt: Q9DGR6 Zebrafish
  • SwissProt: Q9DGR5 Zebrafish
  • Unigene: 861 Human
  • Unigene: 431850 Human
  • Unigene: 196581 Mouse
  • Unigene: 8385 Mouse
  • Unigene: 2592 Rat
  • Unigene: 34914 Rat
  • Unigene: 1680 Xenopus laevis
  • Unigene: 874 Xenopus laevis
  • Unigene: 10452 Zebrafish
  • Unigene: 75913 Zebrafish

  see all

• Alternative names
  ERK 1 antibodyERK 2 antibodyERK antibody

  ERK-2 antibodyERK1 antibodyERK2 antibodyERT1 antibodyERT2 antibodyExtracellular signal regulated kinase 1 antibodyExtracellular signal regulated kinase 2 antibodyExtracellular signal-regulated kinase 2 antibodyHS44KDAP antibodyHUMKER1A antibodyInsulin stimulated MAP2 kinase antibodyMAP kinase 1 antibodyMAP kinase 2 antibodyMAP kinase isoform p42 antibodyMAP kinase isoform p44 antibodyMAPK 1 antibodyMAPK 2 antibodyMAPK 3 antibodyMAPK1 antibodyMAPK2 antibodyMAPK3 antibodyMicrotubule associated protein 2 kinase antibodyMitogen activated protein kinase 1 antibodyMitogen activated protein kinase 2 antibodyMitogen activated protein kinase 3 antibodyMitogen-activated protein kinase 1 antibodyMitogen-activated protein kinase 2 antibodyMK01_HUMAN antibodyp38 antibodyp40 antibodyp41 antibodyp42-MAPK antibodyPRKM 1 antibodyPRKM 2 antibodyPRKM 3 antibodyPRKM1 antibodyPRKM2 antibodyPRKM3 antibody

  see all

Anti-ERK1 + ERK2 (phospho T185 + Y187 + T202 + Y204) antibody [MAPK-YT] images

• 

  Western blot - ERK1 + ERK2 (phospho T183 + Y185) antibody [MAPK-YT] (ab50011)
  Lane 1 : Anti-ERK1 + ERK2 (phospho T185 + Y187 + T202 + Y204) antibody [MAPK-YT] (ab50011) at 1/10000 dilution
  Lane 2 : no primary antibody
  
  Lane 1 : Extract of Rat1 cells activated with 100µM Vanadate+200µM H₂O₂
  Lane 2 : Extract of Rat1 cells activated with 100µM Vanadate+200µM H₂O₂
  
  Secondary
  Goat Anti-Mouse IgG, AlkPhos Conjugate
  
  Predicted band size : 44 kDa
  Observed band size : 42,44 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunocytochemistry - ERK1 + ERK2 (phospho T183 + Y185) antibody [MAPK-YT] (ab50011)
  In situ immunostaining of the activated form of MAP kinase with ab50011 (green) in Drosophila wing imaginal disc (500 micrometers long).