Anti-ERK1 + ERK2 (phospho T185 + Y187 + T202 + Y204) antibody [MAPK-YT] (ab50011)
Overview
- Product nameAnti-ERK1 + ERK2 (phospho T185 + Y187 + T202 + Y204) antibody [MAPK-YT]See all ERK1 + ERK2 primary antibodies ...
- DescriptionMouse monoclonal [MAPK-YT] to ERK1 + ERK2 (phospho T185 + Y187 + T202 + Y204)
- Specificityab50011 is specific for the active, dually-phosphorylated form of MAP kinase (ERK-1 and ERK-2, 44 kDa and 42 kDa, respectively). It does not recognise the non-phosphorylated or the monophosphorylated forms of the MAP kinase molecule or the diphosphorylated forms of JUN kinase and p38 MAP kinase.
- Tested applicationsICC/IF, ELISA, WB, IP, IHC-P more details
- Species reactivityReacts with: Mouse, Rat, Cow, Human, Saccharomyces cerevisiae, Fruit fly (Drosophila melanogaster)
Predicted to work with: Xenopus laevis, Zebrafish - Immunogen
Synthetic peptide HTGFLpTEpYVAT corresponding to the phosphorylated form of human ERK1 and ERK2 activation loop.
- EpitopeThe epitope recognized by ab50011 is located in the region of phosphorylated threonine and tyrosine residues within the regulatory site of active MAP kinase (Thr185 and Tyr187 in ERK-2).
- Positive controlRat brain extract, Drosophila wing imaginal disc.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 15mM Sodium Azide
Constituents: Ascites -
Concentration information loading... - PurityAscites
- Clonality Monoclonal
- Clone numberMAPK-YT
- IsotypeIgG1
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab50011 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | ICC/IF: 1/200. |
| ELISA | ELISA: Use at an assay dependent dilution. |
| WB | WB: 1/10000. Predicted molecular weight: 44 kDa. |
| IP | IP: Use at an assay dependent dilution. |
| IHC-P | IHC-P: Use at an assay dependent dilution. |
Target
- FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity. - Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. - DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
- Post-translational
modificationsDually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187. - Cellular localizationNucleus.
-
Database links
- Entrez Gene: 327672 Cow
- Entrez Gene: 5595 Human
- Entrez Gene: 5594 Human
- Entrez Gene: 26417 Mouse
- Entrez Gene: 26413 Mouse
- Entrez Gene: 50689 Rat
- Entrez Gene: 116590 Rat
- Entrez Gene: 398985 Xenopus laevis
- Entrez Gene: 399480 Zebrafish
- Omim: 601795 Human
- Omim: 176948 Human
- SwissProt: P46196 Cow
- SwissProt: P27361 Human
- SwissProt: P28482 Human
- SwissProt: Q63844 Mouse
- SwissProt: P63085 Mouse
- SwissProt: P21708 Rat
- SwissProt: P63086 Rat
- SwissProt: P26696 Xenopus laevis
- SwissProt: Q9DGR6 Zebrafish
- SwissProt: Q9DGR5 Zebrafish
- Unigene: 861 Human
- Unigene: 431850 Human
- Unigene: 196581 Mouse
- Unigene: 8385 Mouse
- Unigene: 2592 Rat
- Unigene: 34914 Rat
- Unigene: 1680 Xenopus laevis
- Unigene: 874 Xenopus laevis
- Unigene: 10452 Zebrafish
- Unigene: 75913 Zebrafish
see all
Target information above from: UniProt accession
P28482
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- ERK 1 antibodyERK 2 antibodyERK antibody
- ERK-2 antibodyERK1 antibodyERK2 antibodyERT1 antibodyERT2 antibodyExtracellular signal regulated kinase 1 antibodyExtracellular signal regulated kinase 2 antibodyExtracellular signal-regulated kinase 2 antibodyHS44KDAP antibodyHUMKER1A antibodyInsulin stimulated MAP2 kinase antibodyMAP kinase 1 antibodyMAP kinase 2 antibodyMAP kinase isoform p42 antibodyMAP kinase isoform p44 antibodyMAPK 1 antibodyMAPK 2 antibodyMAPK 3 antibodyMAPK1 antibodyMAPK2 antibodyMAPK3 antibodyMicrotubule associated protein 2 kinase antibodyMitogen activated protein kinase 1 antibodyMitogen activated protein kinase 2 antibodyMitogen activated protein kinase 3 antibodyMitogen-activated protein kinase 1 antibodyMitogen-activated protein kinase 2 antibodyMK01_HUMAN antibodyp38 antibodyp40 antibodyp41 antibodyp42-MAPK antibodyPRKM 1 antibodyPRKM 2 antibodyPRKM 3 antibodyPRKM1 antibodyPRKM2 antibodyPRKM3 antibody
see all
Anti-ERK1 + ERK2 (phospho T185 + Y187 + T202 + Y204) antibody [MAPK-YT] images
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Lane 1 : Anti-ERK1 + ERK2 (phospho T185 + Y187 + T202 + Y204) antibody [MAPK-YT] (ab50011) at 1/10000 dilution
Lane 2 : no primary antibody
Lane 1 : Extract of Rat1 cells activated with 100µM Vanadate+200µM H2O2
Lane 2 : Extract of Rat1 cells activated with 100µM Vanadate+200µM H2O2
Secondary
Goat Anti-Mouse IgG, AlkPhos Conjugate
Predicted band size : 44 kDa
Observed band size : 42,44 kDa (why is the actual band size different from the predicted?) -
In situ immunostaining of the activated form of MAP kinase with ab50011 (green) in Drosophila wing imaginal disc (500 micrometers long).
References for Anti-ERK1 + ERK2 (phospho T185 + Y187 + T202 + Y204) antibody [MAPK-YT] (ab50011)
This product has been referenced in:
- Knezevic I et al. A Novel Cardiomyocyte-enriched MicroRNA, miR-378, Targets Insulin-like Growth Factor 1 Receptor: IMPLICATIONS IN POSTNATAL CARDIAC REMODELING AND CELL SURVIVAL. J Biol Chem 287:12913-26 (2012). WB ; Rat . Read more (PubMed: 22367207) »
- Tummala H et al. The D153del Mutation in GNB3 Gene Causes Tissue Specific Signalling Patterns and an Abnormal Renal Morphology in Rge Chickens. PLoS One 6:e21156 (2011). WB ; Chicken . Read more (PubMed: 21887213) »
![Western blot - ERK1 + ERK2 (phospho T183 + Y185) antibody [MAPK-YT] (ab50011) Western blot - ERK1 + ERK2 (phospho T183 + Y185) antibody [MAPK-YT] (ab50011)](http://a.abcam.com/ps/datasheet/Images/50/ab50011/ab50011wb copy.gif)
![Immunocytochemistry - ERK1 + ERK2 (phospho T183 + Y185) antibody [MAPK-YT] (ab50011) Immunocytochemistry - ERK1 + ERK2 (phospho T183 + Y185) antibody [MAPK-YT] (ab50011)](http://a.abcam.com/ps/datasheet/Images/50/ab50011/ab50011if.jpg)

