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Anti-ERK1 + ERK2 (phospho T185 + Y187 + T202 + Y204) antibody [MAPK-YT] (ab50011)

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Overview

Product name

Anti-ERK1 + ERK2 (phospho T185 + Y187 + T202 + Y204) antibody [MAPK-YT]
See all ERK1 + ERK2 products (22) ...

Description

Mouse monoclonal [MAPK-YT] to ERK1 + ERK2 (phospho T185 + Y187 + T202 + Y204)

Specificity

ab50011 is specific for the active, dually-phosphorylated form of MAP kinase (ERK-1 and ERK-2, 44 kDa and 42 kDa, respectively). It does not recognise the non-phosphorylated or the monophosphorylated forms of the MAP kinase molecule or the diphosphorylated forms of JUN kinase and p38 MAP kinase.

Tested applications

ICC/IF, ELISA, WB, IP, IHC-Pmore details

Cross reactivity

Reacts with

Mouse, Rat, Cow, Human, Saccharomyces cerevisiae, Fruit fly (Drosophila melanogaster)

Predicted to work with

Xenopus laevis, Zebrafish

Immunogen

Synthetic peptide HTGFLpTEpYVAT corresponding to the phosphorylated form of human ERK1 and ERK2 activation loop.

HTGFLTEYVAT

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Epitope

The epitope recognized by ab50011 is located in the region of phosphorylated threonine and tyrosine residues within the regulatory site of active MAP kinase (Thr185 and Tyr187 in ERK-2).

Positive control

Rat brain extract, Drosophila wing imaginal disc.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: Ascites

Concentration

Concentration information loading...

Purity

Ascites

Clonality

Monoclonal

Clone number

MAPK-YT

Isotype

IgG1

Research areas

Stem Cells >> Signaling Pathways >> TGF beta >> Cytoplasmic
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> MAPK Pathway
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Tangles & Tau

  • Western blot - ERK1 + ERK2 (phospho T183 + Y185) antibody [MAPK-YT] (ab50011)Western blot - ERK1 + ERK2 (phospho T183 + Y185) antibody [MAPK-YT] (ab50011) image (enlarge)

  • Immunocytochemistry - ERK1 + ERK2 (phospho T183 + Y185) antibody [MAPK-YT] (ab50011)Immunocytochemistry - ERK1 + ERK2 (phospho T183 + Y185) antibody [MAPK-YT] (ab50011) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab50011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational
modifications

Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.

Cellular localization

Nucleus.

Target information above from: UniProt accessionP28482 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • ERK 1 antibody
  • ERK 2 antibody
  • ERK antibody
  • ERK-2 antibody
  • ERK1 antibody
  • ERK2 antibody
  • ERT1 antibody
  • ERT2 antibody
  • Extracellular signal regulated kinase 1 antibody
  • Extracellular signal regulated kinase 2 antibody
  • Extracellular signal-regulated kinase 2 antibody
  • HS44KDAP antibody
  • HUMKER1A antibody
  • Insulin stimulated MAP2 kinase antibody
  • MAP kinase 1 antibody
  • MAP kinase 2 antibody
  • MAP kinase isoform p42 antibody
  • MAP kinase isoform p44 antibody
  • MAPK 1 antibody
  • MAPK 2 antibody
  • MAPK 3 antibody
  • MAPK1 antibody
  • MAPK2 antibody
  • MAPK3 antibody
  • Microtubule associated protein 2 kinase antibody
  • Mitogen activated protein kinase 1 antibody
  • Mitogen activated protein kinase 2 antibody
  • Mitogen activated protein kinase 3 antibody
  • Mitogen-activated protein kinase 1 antibody
  • Mitogen-activated protein kinase 2 antibody
  • MK01_HUMAN antibody
  • p38 antibody
  • p40 antibody
  • p41 antibody
  • p42-MAPK antibody
  • PRKM 1 antibody
  • PRKM 2 antibody
  • PRKM 3 antibody
  • PRKM1 antibody
  • PRKM2 antibody
  • PRKM3 antibody
see all

Database links

see all

Anti-ERK1 + ERK2 (phospho T185 + Y187 + T202 + Y204) antibody [MAPK-YT] images:

  Western blot - ERK1 + ERK2 (phospho T183 + Y185) antibody [MAPK-YT] (ab50011)

Western blot - ERK1 + ERK2 (phospho T183 + Y185) antibody [MAPK-YT] (ab50011)

Lane 1 : Anti-ERK1 + ERK2 (phospho T185 + Y187 + T202 + Y204) antibody [MAPK-YT] (ab50011) at 1/10000 dilution
Lane 2 : no primary antibody

Lane 1 : Extract of Rat1 cells activated with 100µM Vanadate+200µM H2O2
Lane 2 : Extract of Rat1 cells activated with 100µM Vanadate+200µM H2O2

Secondary
Goat Anti-Mouse IgG, AlkPhos Conjugate

Predicted band size : 44 kDa
Observed band size : 42,44 kDa (why is the actual band size different from the predicted?)

  Immunocytochemistry - ERK1 + ERK2 (phospho T183 + Y185) antibody [MAPK-YT] (ab50011)

Immunocytochemistry - ERK1 + ERK2 (phospho T183 + Y185) antibody [MAPK-YT] (ab50011)

In situ immunostaining of the activated form of MAP kinase with ab50011 (green) in Drosophila wing imaginal disc (500 micrometers long).

References for Anti-ERK1 + ERK2 (phospho T185 + Y187 + T202 + Y204) antibody [MAPK-YT] (ab50011)

This product has been referenced in:

  • Knezevic I  et al. A Novel Cardiomyocyte-enriched MicroRNA, miR-378, Targets Insulin-like Growth Factor 1 Receptor: IMPLICATIONS IN POSTNATAL CARDIAC REMODELING AND CELL SURVIVAL. J Biol Chem 287:12913-26 (2012). WB; Rat.Read more (PubMed: 22367207) »
  • Tummala H  et al. The D153del Mutation in GNB3 Gene Causes Tissue Specific Signalling Patterns and an Abnormal Renal Morphology in Rge Chickens. PLoS One 6:e21156 (2011). WB; Chicken.Read more (PubMed: 21887213) »

See all 9 publications for this product

Publishing research using ab50011? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"