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Read our guarantee »Anti-ERK1 + ERK2 (phospho T185 + Y187 + T202 + Y204) antibody [MAPK-YT]
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Mouse monoclonal [MAPK-YT] to ERK1 + ERK2 (phospho T185 + Y187 + T202 + Y204)
ab50011 is specific for the active, dually-phosphorylated form of MAP kinase (ERK-1 and ERK-2, 44 kDa and 42 kDa, respectively). It does not recognise the non-phosphorylated or the monophosphorylated forms of the MAP kinase molecule or the diphosphorylated forms of JUN kinase and p38 MAP kinase.
ICC/IF, ELISA, WB, IP, IHC-Pmore details
Reacts with
Mouse, Rat, Cow, Human, Saccharomyces cerevisiae, Fruit fly (Drosophila melanogaster)
Predicted to work with
Xenopus laevis, Zebrafish
Synthetic peptide HTGFLpTEpYVAT corresponding to the phosphorylated form of human ERK1 and ERK2 activation loop.
HTGFLTEYVAT
The epitope recognized by ab50011 is located in the region of phosphorylated threonine and tyrosine residues within the regulatory site of active MAP kinase (Thr185 and Tyr187 in ERK-2).
Rat brain extract, Drosophila wing imaginal disc.
Liquid
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 15mM Sodium Azide
Constituents: Ascites
Concentration information loading...
Ascites
Monoclonal
MAPK-YT
IgG1
Stem Cells >> Signaling Pathways >> TGF beta >> Cytoplasmic
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> MAPK Pathway
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Tangles & Tau
Our Abpromise guarantee covers the use of ab50011 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/200.
ELISA: Use at an assay dependent dilution.
WB: 1/10000. Predicted molecular weight: 44 kDa.
IP: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution.
Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
Nucleus.
Target information above from: UniProt accessionP28482
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - ERK1 + ERK2 (phospho T183 + Y185) antibody [MAPK-YT] (ab50011)
![Western blot - ERK1 + ERK2 (phospho T183 + Y185) antibody [MAPK-YT] (ab50011)](/ps/datasheet/Images/50/ab50011/ab50011wb copy.gif)
Lane 1 : Anti-ERK1 + ERK2 (phospho T185 + Y187 + T202 + Y204) antibody [MAPK-YT] (ab50011) at 1/10000 dilution
Lane 2 : no primary antibody
Lane 1 : Extract of Rat1 cells activated with 100µM
Vanadate+200µM H2O2
Lane 2 : Extract of Rat1 cells activated with 100µM
Vanadate+200µM H2O2
Secondary
Goat Anti-Mouse IgG, AlkPhos
Conjugate
Predicted band size : 44 kDa
Observed band size : 42,44 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry - ERK1 + ERK2 (phospho T183 + Y185) antibody [MAPK-YT] (ab50011)
![Immunocytochemistry - ERK1 + ERK2 (phospho T183 + Y185) antibody [MAPK-YT] (ab50011)](/ps/datasheet/Images/50/ab50011/ab50011if.jpg)
In situ immunostaining of the activated form of MAP kinase with ab50011 (green) in Drosophila wing imaginal disc (500 micrometers long).
This product has been referenced in:
See all 9 publications for this product
Publishing research using ab50011? Please let us know so that we can cite the reference in this datasheet
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![Immunocytochemistry - ERK1 + ERK2 (phospho T183 + Y185) antibody [MAPK-YT] (ab50011)](/ps/datasheet/Images/50/ab50011/ab50011if.jpg)
In situ immunostaining of the activated form of MAP kinase with ab50011 (green) in Drosophila wing imaginal disc (500 micrometers long).
![ERK1 + ERK2 (phospho T185 + Y187 + T202 + Y204) antibody [MAPK-YT] for Immunocytochemistry/ Immunofluorescence in Mouse (50011)](/ps/datasheet/images/50/ab50011/ERK1-ERK2-Primary-antibodies-ab50011-3.jpg)
![Anti-ERK1 + ERK2 (phospho T185 + Y187 + T202 + Y204) antibody [MAPK-YT] for Western blot in Grasshopper (50011)](/ps/datasheet/images/50/ab50011/ERK1-ERK2-Primary-antibodies-ab50011-11.jpg)
![Anti-ERK1 + ERK2 (phospho T185 + Y187 + T202 + Y204) antibody [MAPK-YT] for Immunocytochemistry/ Immunofluorescence in Mouse (50011)](/ps/datasheet/images/50/ab50011/ERK1-ERK2-Primary-antibodies-ab50011-17.jpg)
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