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Anti-ERK1 + ERK2 (phospho T202 + Y204 + Y185 + Y187) antibody (ab16869)

Abreviews (4)Q&A (6)Specific References (1)

Overview

  • Product nameAnti-ERK1 + ERK2 (phospho T202 + Y204 + Y185 + Y187) antibodySee all ERK1 + ERK2 primary antibodies ...
  • Description
    Rabbit polyclonal to ERK1 + ERK2 (phospho T202 + Y204 + Y185 + Y187)
  • SpecificityThis antibody recognises ERK1 phosphorylated at Thr202 and Tyr204 and ERK2 phosphorylated at Thr185 and Thr187.
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phospholyrated peptide (KLH coupled) corresponding to residues around Thr202/Tyr204 of human p44 MAP kinase (Erk1)

  • Positive controlNIH3T3 cell lysates stimulated with PDGF.

Properties

Applications

Our Abpromise guarantee covers the use of ab16869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/5000. Predicted molecular weight: 43 kDa.

Target

  • Alternative names
      ERK 1 antibodyERK 2 antibodyERK-2 antibody
      ERK1 antibodyERK2 antibodyERT1 antibodyERT2 antibodyExtracellular signal regulated kinase 1 antibodyExtracellular signal regulated kinase 1 antibodyExtracellular signal regulated kinase 2 antibodyExtracellular signal regulated kinase 2 antibodyExtracellular signal-regulated kinase 2 antibodyHS44KDAP antibodyHUMKER1A antibodyInsulin stimulated MAP2 kinase antibodyMAP kinase 1 antibodyMAP kinase 2 antibodyMAP kinase isoform p42 antibodyMAP kinase isoform p44 antibodyMAPK 1 antibodyMAPK 2 antibodyMAPK 3 antibodyMAPK 3 antibodyMAPK1 antibodyMAPK2 antibodyMAPK3 antibodyMGC20180 antibodyMicrotubule associated protein 2 kinase antibodyMitogen activated protein kinase 1 antibodyMitogen activated protein kinase 1 antibodyMitogen activated protein kinase 2 antibodyMitogen activated protein kinase 3 antibodyMitogen activated protein kinase 3 antibodyMitogen-activated protein kinase 1 antibodyMitogen-activated protein kinase 2 antibodyMK01_HUMAN antibodyp38 antibodyp40 antibodyp41 antibodyp41mapk antibodyp42 MAPK antibodyp42-MAPK antibodyp42MAPK antibodyp42MAPK antibodyp44 ERK1 antibodyp44 MAPK antibodyP44ERK1 antibodyP44MAPK antibodyPRKM 1 antibodyPRKM 1 antibodyPRKM 2 antibodyPRKM 2 antibodyPRKM 3 antibodyPRKM 3 antibodyPRKM1 antibodyPRKM2 antibodyPRKM3 antibodyProtein kinase mitogen activated 1 antibodyProtein kinase mitogen activated 1 antibodyProtein kinase mitogen activated 2 antibodyProtein kinase mitogen activated 2 antibodyProtein kinase mitogen activated 3 antibodyProtein kinase mitogen activated 3 antibodyProtein tyrosine kinase ERK 2 antibodyProtein tyrosine kinase ERK 2 antibody
    see all

Anti-ERK1 + ERK2 (phospho T202 + Y204 + Y185 + Y187) antibody images

  • - ERK1 + ERK2 (phospho T202/185 + Y204/187) antibody (ab16869)
    - ERK1 + ERK2 (phospho T202/185 + Y204/187) antibody (ab16869)
    Western Blot analysis of NIH3T3 cell lysates (10µg) stimulated with PDGF

References for Anti-ERK1 + ERK2 (phospho T202 + Y204 + Y185 + Y187) antibody (ab16869)

This product has been referenced in:
  • Velten M  et al. Systemic maternal inflammation and neonatal hyperoxia induces remodeling and left ventricular dysfunction in mice. PLoS One 6:e24544 (2011). WB ; Human . Read more (PubMed: 21935422) »

See 1 Publication for this product

Publishing research using ab16869 ? Please let us know so that we can cite the reference in this datasheet

Product Wall

Submit an Abreview for ab16869 Submit a Question for ab16869

Displaying 1 - 10 of 10 results for Abreviews and Q&A

10
Votes: Highest First

Thank you for letting me know,

If possible could you please send me an email when the lysate is available that would be great! Then I can let the customer know.

Thanks for your help!

Kind regards,

Thank you for your patience in this matter.

I am very pleased to inform you that the product (positive control lysate for ab16869 - NIH3T3 cell lysate stimulated with PDGF) is now available and we are now doing the final internal necessary steps...

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Thank you for letting me know,

If possible could you please send me an email when the lysate is available that would be great! Then I can let the customer know.

Thanks for your help!

Kind regards,

Thank you for your kind reminder.

I will be sending an e-mail as soon as have further information about the lysate from the Lab.

Thank you for your patience in advance.

Please see below the customer’s response;

Thank you for sending the info. The manuscrip didn't use the Phosphor ERK1/ERK2.

I understand that cells need to be stimulated with PDGF. I am wondering whether your company provides th...

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I can now inform you that we do not have the lysate in stock currently. This is expected to be available at the earliest next month.

I am sorry for the inconvenience. If you have any further questions or concerns please do not hesitate to contac...

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Hi Abcam,

Could you please advise which positive control you would recommend for this antibody? As I could not find the one specified on the datasheet.

Thanks for your help!

Kind regards,

Thank you for your enquiry regarding ab16869.

The cells need to be stimulated and in order to be able to detect phosphorylated ERK1 + ERK2 PDGF is recommended as inducer.

Your customer may wish to take a look at this specific publicati...

Read More

Western blot abreview for Anti-ERK1 + ERK2 (phospho T202 + Y204 + Y185 + Y187) antibody

 Poor
Abreviews
Application Western blot
Sample Mouse Cell lysate - whole cell (BV2 cell line)
Loading amount 400000 cells
Specification BV2 cell line
Treatment LPS 50 ng/mL or LPS 50 ng/mL + UO 10 uM
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Verified customer

Submitted Aug 08 2008

Western blot abreview for Anti-ERK1 + ERK2 (phospho T202 + Y204 + Y185 + Y187) antibody

 Below Average
Abreviews
Application Western blot
Sample Rat Tissue lysate - whole (spinal cord homogenate)
Loading amount 20 µg
Specification spinal cord homogenate
Treatment sham or nerve transected
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Verified customer

Submitted Aug 08 2008

Western blot abreview for Anti-ERK1 + ERK2 (phospho T202 + Y204 + Y185 + Y187) antibody

 Below Average
Abreviews
Application Western blot
Sample Rat Cell lysate - whole cell (HAPI cell line)
Loading amount 400000 cells
Specification HAPI cell line
Treatment LPS 50 ng/mL or LPS 50 ng/mL + UO 10 uM
Gel Running Conditions Reduced Denaturing (10 % SDA-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Verified customer

Submitted Jun 11 2008

Western blot abreview for Anti-ERK1 + ERK2 (phospho T202 + Y204 + Y185 + Y187) antibody

 Good
Abreviews
Application Western blot
Sample Rat Cell lysate - whole cell (Primary Microglia)
Loading amount 400000 cells
Specification Primary Microglia
Treatment LPS 50ng/mL or LPS 50 ng/mL + UO 10 uM
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Verified customer

Submitted Jun 09 2008

DESCRIPTION OF THE PROBLEM No signal or weak signal SAMPLE Rat bladder smooth muscle cell total protein extracts PRIMARY ANTIBODY Primary antibody was ab16869 for phospho erk 1/2 produced in rabbit. Antibody was used at a ratio of 1:5000 in the ...

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Thank you for filling out our technical help questionaire so thoroughly. You protocol looks correct, however I advise for this or any other antibody against a phosphorylated protein that you avoid using milk in your diluents and blocking buffer. Milk cont...

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Hi, I was wondering what is the difference between AB 16869 and AB 4819. They both say they are phoso erk 1 and 2. Thank you

Thank you for contacting us with your query. There is only a small difference between these two antibodies if you are going to make a choice between the two. They will both recognise phosphorylated ERK1 and ERK2. ERK1 and ERK2 are phosphorylated within th...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"