Overview
- Product nameAnti-ERK1 + ERK2 (phospho T202 + Y204) antibodySee all ERK1 + ERK2 primary antibodies ...
- DescriptionSheep polyclonal to ERK1 + ERK2 (phospho T202 + Y204)
- Specificityab76166 may also recognise the unphosphorylated forms of ERK1 + ERK2.
- Tested applicationsWB, IHC-P, IHC-Fr more details
- Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Xenopus laevis, Zebrafish - Immunogen
A synthetic peptide from the phospho T202 and Y204 region of human ERK1 + ERK2 conjugated to an immunogenic carrier protein.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50%, aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 1X PBS -
Concentration information loading... - PurityIgG fraction
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab76166 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | |
| IHC-P | |
| IHC-Fr |
IHC-Fr: Use at a concentration of 10 - 50 µg/ml.
WB: Use at a concentration of 10 - 50 µg/ml. Predicted molecular weight: 44 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
- FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity. - Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. - DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
- Post-translational
modificationsDually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187. - Cellular localizationNucleus.
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Database links
- Entrez Gene: 5595 Human
- Entrez Gene: 5594 Human
- Entrez Gene: 26417 Mouse
- Entrez Gene: 26413 Mouse
- Entrez Gene: 50689 Rat
- Entrez Gene: 116590 Rat
- Entrez Gene: 398985 Xenopus laevis
- Entrez Gene: 399480 Zebrafish
- Omim: 601795 Human
- Omim: 176948 Human
- SwissProt: P27361 Human
- SwissProt: P28482 Human
- SwissProt: Q63844 Mouse
- SwissProt: P63085 Mouse
- SwissProt: P21708 Rat
- SwissProt: P63086 Rat
- SwissProt: P26696 Xenopus laevis
- SwissProt: Q9DGR6 Zebrafish
- SwissProt: Q9DGR5 Zebrafish
- Unigene: 861 Human
- Unigene: 431850 Human
- Unigene: 196581 Mouse
- Unigene: 8385 Mouse
- Unigene: 2592 Rat
- Unigene: 34914 Rat
- Unigene: 1680 Xenopus laevis
- Unigene: 874 Xenopus laevis
- Unigene: 10452 Zebrafish
- Unigene: 75913 Zebrafish
see all
Target information above from: UniProt accession
P28482
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- ERK 1 antibodyERK 2 antibodyERK antibody
- ERK-2 antibodyERK1 antibodyERK2 antibodyERT1 antibodyERT2 antibodyExtracellular signal regulated kinase 1 antibodyExtracellular signal regulated kinase 2 antibodyExtracellular signal-regulated kinase 2 antibodyHS44KDAP antibodyHUMKER1A antibodyInsulin stimulated MAP2 kinase antibodyMAP kinase 1 antibodyMAP kinase 2 antibodyMAP kinase isoform p42 antibodyMAP kinase isoform p44 antibodyMAPK 1 antibodyMAPK 2 antibodyMAPK 3 antibodyMAPK1 antibodyMAPK2 antibodyMAPK3 antibodyMGC20180 antibodyMicrotubule associated protein 2 kinase antibodyMitogen activated protein kinase 1 antibodyMitogen activated protein kinase 2 antibodyMitogen activated protein kinase 3 antibodyMitogen-activated protein kinase 1 antibodyMitogen-activated protein kinase 2 antibodyMK01_HUMAN antibodyp38 antibodyp40 antibodyp41 antibodyp41mapk antibodyp42 MAPK antibodyp42-MAPK antibodyp42MAPK antibodyp44 ERK1 antibodyp44 MAPK antibodyp44ERK1 antibodyp44MAPK antibodyPRKM 1 antibodyPRKM 2 antibodyPRKM 3 antibodyPRKM1 antibodyPRKM2 antibodyPRKM3 antibodyProtein kinase mitogen activated 1 antibodyProtein kinase mitogen activated 2 antibodyProtein kinase mitogen activated 3 antibodyProtein tyrosine kinase ERK 2 antibody
see all
References for Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody (ab76166)
ab76166 has not yet been referenced specifically in any publications.
