Anti-ERK1 + ERK2 (phospho Y180) antibody (ab78238)

Overview

• Product nameAnti-ERK1 + ERK2 (phospho Y180) antibodySee all ERK1 + ERK2 primary antibodies ...
• Description
  Sheep polyclonal to ERK1 + ERK2 (phospho Y180)
• SpecificityPhosphorylated ERK1 (42 kDa) and ERK2 (44kDa).
• Tested applicationsWB, IP more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic peptide: CTGFLTEY^pVAT, corresponding to amino acids 174-183 of Human ERK1 + ERK2

  Run BLAST with Run BLAST with
• Positive controlWB: lysates from serum-stimulated mouse NIH/3T3 cells, from NGF-stimulated rat PC-12 cells and EGF-stimulated human A431 carcinoma cells. IP: EGF stimulated A431 RIPA lysate.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.05% Sodium Azide
  Constituents: 30% Glycerol, 0.2M Tris glycine, 0.15M Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Signaling Pathways
  • TGF beta
  • Cytoplasmic

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • MAPK Pathway

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Alzheimer's disease
  • Tangles & Tau

Applications

Target

• FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
  Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
• Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
  Contains 1 protein kinase domain.
• DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
• Post-translational
  modificationsDually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
• Cellular localizationNucleus.

Target information above from: UniProt accession P28482 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5595 Human
  • Entrez Gene: 5594 Human
  • Entrez Gene: 26417 Mouse
  • Entrez Gene: 26413 Mouse
  • Entrez Gene: 50689 Rat
  • Entrez Gene: 116590 Rat
  • Omim: 601795 Human
  • Omim: 176948 Human

  • SwissProt: P27361 Human
  • SwissProt: P28482 Human
  • SwissProt: Q63844 Mouse
  • SwissProt: P63085 Mouse
  • SwissProt: P21708 Rat
  • SwissProt: P63086 Rat
  • Unigene: 861 Human
  • Unigene: 431850 Human
  • Unigene: 196581 Mouse
  • Unigene: 8385 Mouse
  • Unigene: 2592 Rat
  • Unigene: 34914 Rat

  see all

• Alternative names
  ERK 1 antibodyERK 2 antibodyERK antibody

  ERK-2 antibodyERK1 antibodyERK2 antibodyERT1 antibodyERT2 antibodyExtracellular signal regulated kinase 1 antibodyExtracellular signal regulated kinase 2 antibodyExtracellular signal-regulated kinase 2 antibodyHS44KDAP antibodyHUMKER1A antibodyInsulin stimulated MAP2 kinase antibodyMAP kinase 1 antibodyMAP kinase 2 antibodyMAP kinase isoform p42 antibodyMAP kinase isoform p44 antibodyMAPK 1 antibodyMAPK 2 antibodyMAPK 3 antibodyMAPK1 antibodyMAPK2 antibodyMAPK3 antibodyMGC20180 antibodyMicrotubule associated protein 2 kinase antibodyMitogen activated protein kinase 1 antibodyMitogen activated protein kinase 2 antibodyMitogen activated protein kinase 3 antibodyMitogen-activated protein kinase 1 antibodyMitogen-activated protein kinase 2 antibodyMK01_HUMAN antibodyp38 antibodyp40 antibodyp41 antibodyp41mapk antibodyp42 MAPK antibodyp42-MAPK antibodyp42MAPK antibodyp44 ERK1 antibodyp44 MAPK antibodyp44ERK1 antibodyp44MAPK antibodyPRKM 1 antibodyPRKM 2 antibodyPRKM 3 antibodyPRKM1 antibodyPRKM2 antibodyPRKM3 antibodyProtein kinase mitogen activated 1 antibodyProtein kinase mitogen activated 2 antibodyProtein kinase mitogen activated 3 antibodyProtein tyrosine kinase ERK 2 antibody

  see all

Anti-ERK1 + ERK2 (phospho Y180) antibody images

• 

  Western blot - ERK1 + ERK2 (phospho Y180) antibody (ab78238)
  All lanes : Anti-ERK1 + ERK2 (phospho Y180) antibody (ab78238) at 2 µg/ml
  
  Lane 1 : Lysate from serum-stimulated 3T3 cells
  Lane 2 : Lysate from non-stimulated 3T3 cells
  
  Secondary
  Rabbit anti-Sheep, HRP-conjugated at 1/1000 dilution
  developed using the ECL technique
  
  Predicted band size : 42 , 44 kDa
  Observed band size : 42,44 kDa