Anti-ERK1 + ERK2 (phospho Y204 + Y187) antibody (ab47339)

Overview

• Product nameAnti-ERK1 + ERK2 (phospho Y204 + Y187) antibodySee all ERK1 + ERK2 primary antibodies ...
• Description
  Rabbit polyclonal to ERK1 + ERK2 (phospho Y204 + Y187)
• SpecificityThis antibody is specific for ERK1, only when phosphorylated at tyrosine 204.
• Tested applicationsIHC-P, WB, ELISA more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat
• Immunogen

  A synthesized phosphopeptide derived around the phosphorylation site of ERK1 tyrosine 204 and ERK2 tyrosine 187 (T-E-Y_P-V-A).

• Positive controlExtracts from Jurkat cells

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Signaling Pathways
  • TGF beta
  • Cytoplasmic

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • MAPK Pathway

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Alzheimer's disease
  • Tangles & Tau

Applications

Target

• FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
  Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
• Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
  Contains 1 protein kinase domain.
• DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
• Post-translational
  modificationsDually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
• Cellular localizationNucleus.

Target information above from: UniProt accession P28482 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5595 Human
  • Entrez Gene: 5594 Human
  • Entrez Gene: 26417 Mouse
  • Entrez Gene: 26413 Mouse
  • Entrez Gene: 50689 Rat
  • Entrez Gene: 116590 Rat
  • Omim: 601795 Human
  • Omim: 176948 Human

  • SwissProt: P27361 Human
  • SwissProt: P28482 Human
  • SwissProt: Q63844 Mouse
  • SwissProt: P63085 Mouse
  • SwissProt: P21708 Rat
  • SwissProt: P63086 Rat
  • Unigene: 861 Human
  • Unigene: 431850 Human
  • Unigene: 196581 Mouse
  • Unigene: 8385 Mouse
  • Unigene: 2592 Rat
  • Unigene: 34914 Rat

  see all

• Alternative names
  ERK 1 antibodyERK 2 antibodyERK-2 antibody

  ERK1 antibodyERK2 antibodyERT1 antibodyERT2 antibodyExtracellular signal regulated kinase 1 antibodyExtracellular signal-regulated kinase 2 antibodyMAP kinase 1 antibodyMAP kinase 2 antibodyMAP kinase isoform p42 antibodyMAP kinase isoform p44 antibodyMAPK 1 antibodyMAPK 2 antibodyMAPK 3 antibodyMAPK1 antibodyMAPK2 antibodyMAPK3 antibodyMitogen-activated protein kinase 1 antibodyMitogen-activated protein kinase 2 antibodyMK01_HUMAN antibodyp38 antibodyp40 antibodyp41 antibodyp42-MAPK antibodyPRKM 2 antibody

  see all

Anti-ERK1 + ERK2 (phospho Y204 + Y187) antibody images

• 

  Western blot - ERK1 (phospho Y204) antibody (ab47339)
  All lanes : Anti-ERK1 + ERK2 (phospho Y204 + Y187) antibody (ab47339) at 1/500 dilution
  
  Lane 1 : Extracts from Jurkat cells treated with PMA (200ng/ml, 15min)
  Lane 2 : Extracts from Jurkat cells un-treated
  
  
  Predicted band size : 44, 42 kDa
  Observed band size : 42,44 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-ERK1 (phospho Y204) antibody(ab47339)
  Ab47339 staining human normal or colon tissue. Staining is localised to cytoplasm.
  Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
  Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
• 

  Western blot - ERK1 + ERK2 (phospho Y204 + Y187) antibody (ab47339)
  All lanes : Anti-ERK1 + ERK2 (phospho Y204 + Y187) antibody (ab47339)
  
  Lane 1 : Jurkat cell extract
  Lane 2 : Jurkat cell extract treated with PMA
  
  
  Predicted band size : 44, 42 kDa
  Observed band size : 43 kDa (why is the actual band size different from the predicted?)