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Overview

  • Product nameAnti-ERK1 antibody [CT]See all ERK1 primary antibodies ...
  • Description
    Rabbit monoclonal [CT] to ERK1
  • Tested applicationsWB, IP more details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Human, Chinese Hamster
  • Immunogen

    Synthetic peptide: CGGPFTFDME LDDLPKERLK ELIFQETARF QPGAPEAP, corresponding to amino acids 346-380 of Rat ERK1.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control3T3/NIH cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab77759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
IP
  • Application notesIP Kinase Assay: 1-10µl.
    WB: 1/1000 - 1/5000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
    • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
      Contains 1 protein kinase domain.
    • DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
    • Post-translational
      modifications      Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-204.

      • Target information above from: UniProt accession P27361 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      see all
    • Alternative names
        ERK 1 antibodyERK antibodyERK-1 antibody
        ERK1 antibodyERT 2 antibodyERT2 antibodyExtracellular Signal Regulated Kinase 1 antibodyExtracellular signal related kinase 1 antibodyExtracellular signal-regulated kinase 1 antibodyHGNC6877 antibodyHS44KDAP antibodyHUMKER1A antibodyInsulin Stimulated MAP2 Kinase antibodyInsulin-stimulated MAP2 kinase antibodyMAP kinase 1 antibodyMAP kinase 3 antibodyMAP Kinase antibodyMAP kinase isoform p44 antibodyMAPK 1 antibodyMAPK 3 antibodyMAPK antibodyMAPK1 antibodyMAPK3 antibodyMGC20180 antibodyMicrotubule Associated Protein 2 Kinase antibodyMicrotubule-associated protein 2 kinase antibodyMitogen Activated Protein Kinase 3 antibodyMitogen-activated protein kinase 1 antibodyMitogen-activated protein kinase 3 antibodyMK03_HUMAN antibodyOTTHUMP00000174538 antibodyOTTHUMP00000174541 antibodyp44 ERK1 antibodyp44 MAPK antibodyp44-ERK1 antibodyp44-MAPK antibodyP44ERK1 antibodyP44MAPK antibodyPRKM 3 antibodyPRKM3 antibodyProtein Kinase Mitogen Activated 3 antibody
      see all

    Anti-ERK1 antibody [CT] images

    • Western blot - ERK1 antibody [CT] (ab77759)
      Western blot - ERK1 antibody [CT] (ab77759)
      Anti-ERK1 antibody [CT] (ab77759) at 1/1000 dilution + RIPA lysates of 3T3/NIH

      Secondary
      Goat anti-rabbit secondary antibody conjugated to HRP
      developed using the ECL technique

      Predicted band size : 43 kDa
      Observed band size : 43 kDa

    References for Anti-ERK1 antibody [CT] (ab77759)

    ab77759 has not yet been referenced specifically in any publications.

    Publishing research using ab77759 ? Please let us know so that we can cite the reference in this datasheet

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