Anti-ERK1 antibody [E32] (ab73270)

Overview

• Product nameAnti-ERK1 antibody [E32]See all ERK1 primary antibodies ...
• Description
  Mouse monoclonal [E32] to ERK1
• SpecificityRecognizes only the ERK1 protein (e.g.: from Human and mouse cells)
• Tested applicationsWB, IP more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  Recombinant full length human, active, ERK1 protein.

• Positive control293 and HeLa cell lysates.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferpH: 7.20
  Preservative: 0.05% Sodium azide
  Constituent: 0.0268% PBS
• Concentration information loading...
• PurityProtein A purified
• Clonality Monoclonal
• Clone numberE32
• IsotypeIgG1
• Research Areas
  • Stem Cells
  • Signaling Pathways
  • TGF beta
  • Cytoplasmic

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • MAPK Pathway

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Alzheimer's disease
  • Tangles & Tau

Applications

Target

• FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
• Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
  Contains 1 protein kinase domain.
• DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
• Post-translational
  modificationsDually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-204.

Target information above from: UniProt accession P27361 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5595 Human
  • Entrez Gene: 26417 Mouse
  • Omim: 601795 Human
  • SwissProt: P27361 Human
  • SwissProt: Q63844 Mouse
  • Unigene: 861 Human
  • Unigene: 8385 Mouse

• Alternative names
  ERK 1 antibodyERK antibodyERK-1 antibody

  ERK1 antibodyERT 2 antibodyERT2 antibodyExtracellular Signal Regulated Kinase 1 antibodyExtracellular signal related kinase 1 antibodyExtracellular signal-regulated kinase 1 antibodyHGNC6877 antibodyHS44KDAP antibodyHUMKER1A antibodyInsulin Stimulated MAP2 Kinase antibodyInsulin-stimulated MAP2 kinase antibodyMAP kinase 1 antibodyMAP kinase 3 antibodyMAP Kinase antibodyMAP kinase isoform p44 antibodyMAPK 1 antibodyMAPK 3 antibodyMAPK antibodyMAPK1 antibodyMAPK3 antibodyMGC20180 antibodyMicrotubule Associated Protein 2 Kinase antibodyMicrotubule-associated protein 2 kinase antibodyMitogen Activated Protein Kinase 3 antibodyMitogen-activated protein kinase 1 antibodyMitogen-activated protein kinase 3 antibodyMK03_HUMAN antibodyOTTHUMP00000174538 antibodyOTTHUMP00000174541 antibodyp44 ERK1 antibodyp44 MAPK antibodyp44-ERK1 antibodyp44-MAPK antibodyP44ERK1 antibodyP44MAPK antibodyPRKM 3 antibodyPRKM3 antibodyProtein Kinase Mitogen Activated 3 antibody

  see all

Anti-ERK1 antibody [E32] images

• 

  Western blot - ERK1 antibody [E32] (ab73270)
  All lanes : Anti-ERK1 antibody [E32] (ab73270) at 1/1000 dilution
  
  Lane 1 : 293 cell lysate at 5 µg
  Lane 2 : 293 cell lysate at 10 µg
  Lane 3 : 293 cell lysate at 30 µg
  
  
  Predicted band size : 43 kDa
  Observed band size : 43 kDa
  
• 

  Immunoprecipitation - Anti-ERK1 antibody [E32] (ab73270)
  Detection of ERK1 by Western Blot of Immunprecipitate.
  ab73270 staining ERK1 in HeLa whole cell lysate immunoprecipitated using 1µl of ab73270.
  Lane 1: 10µg HeLa lysate.
  Lane 2: 25µg HeLa lysate.
  Lane 3: 50µg HeLa lysate.