Overview

  • Product nameAnti-ERK1 antibody [EP4967]See all ERK1 primary antibodies ...
  • Description
    Rabbit monoclonal [EP4967] to ERK1
  • Tested applicationsWB, IHC-P more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to a region within Human ERK1

  • Positive control
    • Jurkat, A375, A431 and HUVEC cell lysates. Human colon adenocarcinoma tissue.
  • General notes

    Produced under U.S. Patent No. 5,675,063. A 40 μl trial size is available to purchase for this antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109282 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
  • Application notesIs unsuitable for Flow Cyt,ICC or IP.
  • Target

    • FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
    • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
      Contains 1 protein kinase domain.
    • DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
    • Post-translational
      modifications
      Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-204.
    • Target information above from: UniProt accession P27361 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • ERK 1 antibody
      • ERK antibody
      • ERK-1 antibody
      • ERK1 antibody
      • ERT 2 antibody
      • ERT2 antibody
      • Extracellular Signal Regulated Kinase 1 antibody
      • Extracellular signal related kinase 1 antibody
      • Extracellular signal-regulated kinase 1 antibody
      • HGNC6877 antibody
      • HS44KDAP antibody
      • HUMKER1A antibody
      • Insulin Stimulated MAP2 Kinase antibody
      • Insulin-stimulated MAP2 kinase antibody
      • MAP kinase 1 antibody
      • MAP kinase 3 antibody
      • MAP Kinase antibody
      • MAP kinase isoform p44 antibody
      • MAPK 1 antibody
      • MAPK 3 antibody
      • MAPK antibody
      • MAPK1 antibody
      • Mapk3 antibody
      • MGC20180 antibody
      • Microtubule Associated Protein 2 Kinase antibody
      • Microtubule-associated protein 2 kinase antibody
      • Mitogen Activated Protein Kinase 3 antibody
      • Mitogen-activated protein kinase 1 antibody
      • Mitogen-activated protein kinase 3 antibody
      • MK03_HUMAN antibody
      • OTTHUMP00000174538 antibody
      • OTTHUMP00000174541 antibody
      • p44 ERK1 antibody
      • p44 MAPK antibody
      • p44-ERK1 antibody
      • p44-MAPK antibody
      • P44ERK1 antibody
      • P44MAPK antibody
      • PRKM 3 antibody
      • PRKM3 antibody
      • Protein Kinase Mitogen Activated 3 antibody
      see all

    Anti-ERK1 antibody [EP4967] images

    • All lanes : Anti-ERK1 antibody [EP4967] (ab109282) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : A375 cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : HUVEC cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 44 kDa
    • Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue using ab109282 at 1/100 dilution.

    References for Anti-ERK1 antibody [EP4967] (ab109282)

    ab109282 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"