• Product nameAnti-ERK1 antibody [EP4967]
    See all ERK1 primary antibodies
  • Description
    Rabbit monoclonal [EP4967] to ERK1
  • Tested applicationsWB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to a region within Human ERK1

  • Positive control
    • Jurkat, A375, A431 and HUVEC cell lysates. Human colon adenocarcinoma tissue.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A 40 µl trial size is available to purchase for this antibody.



Our Abpromise guarantee covers the use of ab109282 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
  • Application notesIs unsuitable for Flow Cyt,ICC or IP.
  • Target

    • FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
    • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
      Contains 1 protein kinase domain.
    • DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
    • Post-translational
      Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-204.
    • Information by UniProt
    • Database links
    • Alternative names
      • ERK 1 antibody
      • ERK antibody
      • ERK-1 antibody
      • ERK1 antibody
      • ERT 2 antibody
      • ERT2 antibody
      • Extracellular Signal Regulated Kinase 1 antibody
      • Extracellular signal related kinase 1 antibody
      • Extracellular signal-regulated kinase 1 antibody
      • HGNC6877 antibody
      • HS44KDAP antibody
      • HUMKER1A antibody
      • Insulin Stimulated MAP2 Kinase antibody
      • Insulin-stimulated MAP2 kinase antibody
      • MAP kinase 1 antibody
      • MAP kinase 3 antibody
      • MAP Kinase antibody
      • MAP kinase isoform p44 antibody
      • MAPK 1 antibody
      • MAPK 3 antibody
      • MAPK antibody
      • MAPK1 antibody
      • Mapk3 antibody
      • MGC20180 antibody
      • Microtubule Associated Protein 2 Kinase antibody
      • Microtubule-associated protein 2 kinase antibody
      • Mitogen Activated Protein Kinase 3 antibody
      • Mitogen-activated protein kinase 1 antibody
      • Mitogen-activated protein kinase 3 antibody
      • MK03_HUMAN antibody
      • OTTHUMP00000174538 antibody
      • OTTHUMP00000174541 antibody
      • p44 ERK1 antibody
      • p44 MAPK antibody
      • p44-ERK1 antibody
      • p44-MAPK antibody
      • P44ERK1 antibody
      • P44MAPK antibody
      • PRKM 3 antibody
      • PRKM3 antibody
      • Protein Kinase Mitogen Activated 3 antibody
      see all

    Anti-ERK1 antibody [EP4967] images

    • All lanes : Anti-ERK1 antibody [EP4967] (ab109282) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : A375 cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : HUVEC cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 44 kDa
    • Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue using ab109282 at 1/100 dilution.

    References for Anti-ERK1 antibody [EP4967] (ab109282)

    ab109282 has not yet been referenced specifically in any publications.

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