Overview

  • Product nameAnti-ERK1 antibody [M233]See all ERK1 primary antibodies ...
  • Description
    Mouse monoclonal [M233] to ERK1
  • SpecificityDoes not bind to ERK2
  • Tested applicationsWB, ICC/IF, ELISA, IHC-P more details
  • Species reactivity
    Reacts with: Rabbit, Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to the C terminal region of Human ERK1

  • Positive control
    • ICC/IF: Rabbit spleen fibroblasts. WB: lysate from A431 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab88480 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
ICC/IF 1/100.
ELISA 1/2000.
IHC-P Use at an assay dependent dilution.

Target

  • FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications
    Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-204.
  • Target information above from: UniProt accession P27361 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • ERK 1 antibody
    • ERK antibody
    • ERK-1 antibody
    • ERK1 antibody
    • ERT 2 antibody
    • ERT2 antibody
    • Extracellular Signal Regulated Kinase 1 antibody
    • Extracellular signal related kinase 1 antibody
    • Extracellular signal-regulated kinase 1 antibody
    • HGNC6877 antibody
    • HS44KDAP antibody
    • HUMKER1A antibody
    • Insulin Stimulated MAP2 Kinase antibody
    • Insulin-stimulated MAP2 kinase antibody
    • MAP kinase 1 antibody
    • MAP kinase 3 antibody
    • MAP Kinase antibody
    • MAP kinase isoform p44 antibody
    • MAPK 1 antibody
    • MAPK 3 antibody
    • MAPK antibody
    • MAPK1 antibody
    • MAPK3 antibody
    • MGC20180 antibody
    • Microtubule Associated Protein 2 Kinase antibody
    • Microtubule-associated protein 2 kinase antibody
    • Mitogen Activated Protein Kinase 3 antibody
    • Mitogen-activated protein kinase 1 antibody
    • Mitogen-activated protein kinase 3 antibody
    • MK03_HUMAN antibody
    • OTTHUMP00000174538 antibody
    • OTTHUMP00000174541 antibody
    • p44 ERK1 antibody
    • p44 MAPK antibody
    • p44-ERK1 antibody
    • p44-MAPK antibody
    • P44ERK1 antibody
    • P44MAPK antibody
    • PRKM 3 antibody
    • PRKM3 antibody
    • Protein Kinase Mitogen Activated 3 antibody
    see all

Anti-ERK1 antibody [M233] images

  • All lanes : Anti-ERK1 antibody [M233] (ab88480) at 1/1000 dilution (in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature)

    Lane 1 : Human A431 epithelial cells, untreated
    Lane 2 : Human A431 epithelial cells treated treated with 100 nM calyculin A for 30 minutes
    Lane 3 : Human A431 epithelial cells treated with 100 ng/ml EGF for 60 minutes


    Predicted band size : 43 kDa
    Observed band size : 43 kDa
  • ab88480, at a 1/100 dilution, staining ERK1 in paraformaldehyde fixed and NP-40 permeabilized rabbit spleen fibroblasts by Immunocytochemistry/ Immunofluorescence.
  • Anti-ERK1 antibody [M233] (ab88480) at 1/1000 dilution + Human ERK1 full length protein (ab43623) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds

References for Anti-ERK1 antibody [M233] (ab88480)

ab88480 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (Human Lung Fibroblasts)
Loading amount 30 µg
Specification Human Lung Fibroblasts
Treatment 10ng/ml TGFb1, 10-100µg/ml Nystatin, 5-20µM Cyctochalasin B
Gel Running Conditions Reduced Non-Denaturing (Native) (10% Gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
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Submitted Mar 29 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"