The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain.
DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Post-translational modificationsDually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-204.
All lanes : Anti-ERK1 antibody [M233] (ab88480) at 1/1000 dilution (in 5% non-fat milk, PBS, 0.04% Tween20
for 1hour at room temperature)
Lane 1 : Human A431 epithelial cells,
untreated Lane 2 : Human A431 epithelial cells
treated treated with 100 nM calyculin A
for 30 minutes Lane 3 : Human A431 epithelial cells
treated with 100 ng/ml EGF for 60 minutes
Predicted band size : 43 kDa Observed band size : 43 kDa