Overview

  • Product nameAnti-ERK1 antibody [Y72]
    See all ERK1 primary antibodies
  • Description
    Rabbit monoclonal [Y72] to ERK1
  • Specificityab32537 recognises ERK1. The antibody does not cross-react with other MAP kinases.
  • Tested applicationsWB, ICC, IP, Flow Cyt, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human, Recombinant Fragment
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human ERK1 aa 1-100 (N terminal).
    Database link: P27361

  • Epitopeab32537 reacts with an epitope located in the N terminal region of ERK1.
  • Positive control
    • WB: HeLa, HEK293, Jurkat whole cell lysates + ERK1 Recombinant protein IHC: Lung carcinoma
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A 40 μl trial size is available to purchase for this antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-ERK1 antibody (Alexa Fluor® 488) [Y72] (ab190200)

    Anti-ERK1 antibody (HRP) [Y72] (ab190578)

     

Properties

Applications

Our Abpromise guarantee covers the use of ab32537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 43 kDa).
ICC 1/100.
IP 1/40.
Flow Cyt 1/40. Ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100.

Target

  • FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications
    Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-204.
  • Information by UniProt
  • Database links
  • Alternative names
    • ERK 1 antibody
    • ERK antibody
    • ERK-1 antibody
    • ERK1 antibody
    • ERT 2 antibody
    • ERT2 antibody
    • Extracellular Signal Regulated Kinase 1 antibody
    • Extracellular signal related kinase 1 antibody
    • Extracellular signal-regulated kinase 1 antibody
    • HGNC6877 antibody
    • HS44KDAP antibody
    • HUMKER1A antibody
    • Insulin Stimulated MAP2 Kinase antibody
    • Insulin-stimulated MAP2 kinase antibody
    • MAP kinase 1 antibody
    • MAP kinase 3 antibody
    • MAP Kinase antibody
    • MAP kinase isoform p44 antibody
    • MAPK 1 antibody
    • MAPK 3 antibody
    • MAPK antibody
    • MAPK1 antibody
    • MAPK3 antibody
    • MGC20180 antibody
    • Microtubule Associated Protein 2 Kinase antibody
    • Microtubule-associated protein 2 kinase antibody
    • Mitogen Activated Protein Kinase 3 antibody
    • Mitogen-activated protein kinase 1 antibody
    • Mitogen-activated protein kinase 3 antibody
    • MK03_HUMAN antibody
    • OTTHUMP00000174538 antibody
    • OTTHUMP00000174541 antibody
    • p44 ERK1 antibody
    • p44 MAPK antibody
    • p44-ERK1 antibody
    • p44-MAPK antibody
    • P44ERK1 antibody
    • P44MAPK antibody
    • PRKM 3 antibody
    • PRKM3 antibody
    • Protein Kinase Mitogen Activated 3 antibody
    see all

Anti-ERK1 antibody [Y72] images

  • ab32537 staining ERK1 with ab32537 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 22°C. ab150081, a goat anti rabbit Alexa Fluor® 488 (1/200) was used as the secondary antibody. Counterstained with DAPI. Cytoplasmic and nuclear staining shown.

    See Abreview

  • Anti-ERK1 antibody [Y72] (ab32537) at 1/1000 dilution + Jurkat cell lysate

    Predicted band size : 43 kDa
    Observed band size : 43 kDa
  • All lanes : Anti-ERK1 antibody [Y72] (ab32537) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : Human ERK1 full length protein (ab43623)
    Lane 4 : Human ERK2 full length protein (ab43625)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

    Predicted band size : 43 kDa
    Observed band size : 44 kDa (why is the actual band size different from the predicted?)

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab32537 overnight at 4°C. Antibody binding was detected using a goat anti-rabbit Alexa Fluor 790) ab175781 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

  • ab32537 staining ERK1 in lung carcinoma by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab32537 staining ERK1 (green) in HEK293 cells by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized with 70% methanol. The sample was incubated with the primary antibody (1/40 in PBS + 0.2% BSA + 0.1% sodium azide) for 1 hour at 22°C. A phycoerythrin-conjugated goat anti-rabbit IgG (1/100) was used as the secondary antibody.
    Gating Strategy: Live Cells. Purple plot represents isotype control.

    See Abreview

References for Anti-ERK1 antibody [Y72] (ab32537)

This product has been referenced in:
  • Dakhova O  et al. Global gene expression analysis of reactive stroma in prostate cancer. Clin Cancer Res 15:3979-89 (2009). IHC ; Human . Read more (PubMed: 19509179) »

See 1 Publication for this product

Product Wall

Application Flow Cytometry
Fixation Paraformaldehyde
Permeabilization Yes - 70% methanol
Sample Human Cell (HEK293)
Specification HEK293
Gating Strategy live cells gated
Preparation Cell harvesting/tissue preparation method: Cell dissociation buffer
Sample buffer: Enzyme free
Username

Abcam user community

Verified customer

Submitted Aug 04 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Sample Human Cell (HEK293)
Specification HEK293
Permeabilization Yes - 0.1% Triton X-100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 04 2014

Application Western blot
Loading amount 50000 cells
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Human Cell lysate - whole cell (HEK293)
Specification HEK293
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Aug 04 2014

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Permeabilization Yes - 0.5% Triton X100 in PBS
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

Submitted Mar 27 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"