Anti-ERK1 antibody (ab47470)

Overview

• Product nameAnti-ERK1 antibodySee all ERK1 primary antibodies ...
• Description
  Rabbit polyclonal to ERK1
• SpecificityThs antibody detects endogenous levels of total p44/42 MAP Kinase protein.
• Tested applicationsWB, ELISA more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat
• Immunogen

  A synthesized phosphopeptide derived from human ERK1 around the phosphorylation site of threonine 202 (F-L-T^P-E-Y)

• Positive controlA431 cells

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Signaling Pathways
  • TGF beta
  • Cytoplasmic

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • MAPK Pathway

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Alzheimer's disease
  • Tangles & Tau

Applications

Target

• FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
• Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
  Contains 1 protein kinase domain.
• DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
• Post-translational
  modificationsDually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-204.

Target information above from: UniProt accession P27361 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5595 Human
  • Entrez Gene: 26417 Mouse
  • Entrez Gene: 50689 Rat
  • Omim: 601795 Human
  • SwissProt: P27361 Human
  • SwissProt: Q63844 Mouse
  • SwissProt: P21708 Rat
  • Unigene: 861 Human

  • Unigene: 8385 Mouse
  • Unigene: 2592 Rat

  see all

• Alternative names
  ERK 1 antibodyERK antibodyERK-1 antibody

  ERK1 antibodyERT 2 antibodyERT2 antibodyExtracellular Signal Regulated Kinase 1 antibodyExtracellular signal related kinase 1 antibodyExtracellular signal-regulated kinase 1 antibodyHGNC6877 antibodyHS44KDAP antibodyHUMKER1A antibodyInsulin Stimulated MAP2 Kinase antibodyInsulin-stimulated MAP2 kinase antibodyMAP kinase 1 antibodyMAP kinase 3 antibodyMAP Kinase antibodyMAP kinase isoform p44 antibodyMAPK 1 antibodyMAPK 3 antibodyMAPK antibodyMAPK1 antibodyMAPK3 antibodyMGC20180 antibodyMicrotubule Associated Protein 2 Kinase antibodyMicrotubule-associated protein 2 kinase antibodyMitogen Activated Protein Kinase 3 antibodyMitogen-activated protein kinase 1 antibodyMitogen-activated protein kinase 3 antibodyMK03_HUMAN antibodyOTTHUMP00000174538 antibodyOTTHUMP00000174541 antibodyp44 ERK1 antibodyp44 MAPK antibodyp44-ERK1 antibodyp44-MAPK antibodyP44ERK1 antibodyP44MAPK antibodyPRKM 3 antibodyPRKM3 antibodyProtein Kinase Mitogen Activated 3 antibody

  see all

Anti-ERK1 antibody images

• 

  Western blot - ERK1 antibody (ab47470)
  All lanes : Anti-ERK1 antibody (ab47470) at 1/500 dilution
  
  Lane 1 : A431 cells EGF-treated (200 ng/ml, 30min)
  Lane 2 : A431 cells EGF-treated (200 ng/ml, 30min)
  
  Lysates/proteins at 30 µg per lane.
  
  
  Predicted band size : 43 kDa