Overview

  • Product nameAnti-ERK1 antibodySee all ERK1 primary antibodies ...
  • Description
    Rabbit polyclonal to ERK1
  • SpecificitySpecific for p44 ERK1 and will not react with p42 ERK1. NOTE: We have customer feedback that this antibody may crossreact with ERK2.
  • Tested applicationsIHC-P, WB, IP, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Cow, Dog, Human, Pig
  • Immunogen

    Rat ERK c-terminal peptide conjugated to KLH and BSA.

  • EpitopeThe antigen is based on the C-terminal sequence of rat p44 MAPK (C-LKELIFQETARFQPG-NH2).
  • Positive control
    • Use mouse brain extract for immunoblotting and IP.

Properties

Applications

Our Abpromise guarantee covers the use of ab9363 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 µg/ml.
WB 1/250.
IP Use at 4 µg/mg of lysate.
ELISA 1/500.

Target

  • FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications
    Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-204.
  • Information by UniProt
  • Database links
  • Alternative names
    • ERK 1 antibody
    • ERK antibody
    • ERK-1 antibody
    • ERK1 antibody
    • ERT 2 antibody
    • ERT2 antibody
    • Extracellular Signal Regulated Kinase 1 antibody
    • Extracellular signal related kinase 1 antibody
    • Extracellular signal-regulated kinase 1 antibody
    • HGNC6877 antibody
    • HS44KDAP antibody
    • HUMKER1A antibody
    • Insulin Stimulated MAP2 Kinase antibody
    • Insulin-stimulated MAP2 kinase antibody
    • MAP kinase 1 antibody
    • MAP kinase 3 antibody
    • MAP Kinase antibody
    • MAP kinase isoform p44 antibody
    • MAPK 1 antibody
    • MAPK 3 antibody
    • MAPK antibody
    • MAPK1 antibody
    • MAPK3 antibody
    • MGC20180 antibody
    • Microtubule Associated Protein 2 Kinase antibody
    • Microtubule-associated protein 2 kinase antibody
    • Mitogen Activated Protein Kinase 3 antibody
    • Mitogen-activated protein kinase 1 antibody
    • Mitogen-activated protein kinase 3 antibody
    • MK03_HUMAN antibody
    • OTTHUMP00000174538 antibody
    • OTTHUMP00000174541 antibody
    • p44 ERK1 antibody
    • p44 MAPK antibody
    • p44-ERK1 antibody
    • p44-MAPK antibody
    • P44ERK1 antibody
    • P44MAPK antibody
    • PRKM 3 antibody
    • PRKM3 antibody
    • Protein Kinase Mitogen Activated 3 antibody
    see all

Anti-ERK1 antibody images

  • Ab9363 staining Human normal colon. Staining is localised to the nucleoplasm and cytosol.
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

References for Anti-ERK1 antibody (ab9363)

This product has been referenced in:
  • Llamazares M  et al. The ADAMTS12 metalloproteinase exhibits anti-tumorigenic properties through modulation of the Ras-dependent ERK signalling pathway. J Cell Sci 120:3544-52 (2007). WB ; Dog . Read more (PubMed: 17895370) »
  • Andersson L  et al. PLD1 and ERK2 regulate cytosolic lipid droplet formation. J Cell Sci 119:2246-57 (2006). WB ; Mouse . Read more (PubMed: 16723731) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry. The antibody ab9363 has been tested in direct ELISA, in a protocol such as decribed in the document below. http://www.abcam.com/index.html?pageconfig=resource&rid=11388 I hope this information helps. Please do not...

Read More

Thank you for your enquiry. I am able to provide you with the following information; for some of the antibodies that you are interested in, immunogen information is considered to be proprietary. Ab7948: The sequence is as follows: IFEETARFQPGYRS ...

Read More

I am afraid that we don't carry an antibody against ERK1 that works in immunohistochemistry. However, we are always adding new products and we will keep you informed.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"