Anti-ERK1 antibody (ab9363)
- Product nameAnti-ERK1 antibodySee all ERK1 primary antibodies ...
- DescriptionRabbit polyclonal to ERK1
- SpecificitySpecific for p44 ERK1 and will not react with p42 ERK1. NOTE: We have customer feedback that this antibody may crossreact with ERK2.
- Tested applicationsWB, IP, ELISA more details
- Species reactivityReacts with: Mouse, Rat, Rabbit, Cow, Dog, Human, Pig
Rat ERK c-terminal peptide conjugated to KLH and BSA.
- EpitopeThe antigen is based on the C-terminal sequence of rat p44 MAPK (C-LKELIFQETARFQPG-NH2).
- Positive controlUse mouse brain extract for immunoblotting and IP.
- Storage instructionsStore at +4°C. Do not freeze.
- Storage bufferPBS with 0.02% sodium azide
- Concentration information loading...
- PurityImmunogen affinity purified
- Purification notesImmunoaffinity chromatography with ERK1 terminal peptide on agarose.
- Clonality Polyclonal
- Research Areas
Our Abpromise guarantee covers the use of ab9363 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||IP: Use at 4 µg/mg of lysate.|
- FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
- Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.
- DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
modificationsDually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-204.
- ERK 1 antibodyERK antibodyERK-1 antibody
- ERK1 antibodyERT 2 antibodyERT2 antibodyExtracellular Signal Regulated Kinase 1 antibodyExtracellular signal related kinase 1 antibodyExtracellular signal-regulated kinase 1 antibodyHGNC6877 antibodyHS44KDAP antibodyHUMKER1A antibodyInsulin Stimulated MAP2 Kinase antibodyInsulin-stimulated MAP2 kinase antibodyMAP kinase 1 antibodyMAP kinase 3 antibodyMAP Kinase antibodyMAP kinase isoform p44 antibodyMAPK 1 antibodyMAPK 3 antibodyMAPK antibodyMAPK1 antibodyMAPK3 antibodyMGC20180 antibodyMicrotubule Associated Protein 2 Kinase antibodyMicrotubule-associated protein 2 kinase antibodyMitogen Activated Protein Kinase 3 antibodyMitogen-activated protein kinase 1 antibodyMitogen-activated protein kinase 3 antibodyMK03_HUMAN antibodyOTTHUMP00000174538 antibodyOTTHUMP00000174541 antibodyp44 ERK1 antibodyp44 MAPK antibodyp44-ERK1 antibodyp44-MAPK antibodyP44ERK1 antibodyP44MAPK antibodyPRKM 3 antibodyPRKM3 antibodyProtein Kinase Mitogen Activated 3 antibody
Anti-ERK1 antibody images
Ab9363 staining Human normal colon. Staining is localised to the nucleoplasm and cytosol.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
References for Anti-ERK1 antibody (ab9363)
This product has been referenced in:
- Llamazares M et al. The ADAMTS12 metalloproteinase exhibits anti-tumorigenic properties through modulation of the Ras-dependent ERK signalling pathway. J Cell Sci 120:3544-52 (2007). WB ; Dog . Read more (PubMed: 17895370) »
- Andersson L et al. PLD1 and ERK2 regulate cytosolic lipid droplet formation. J Cell Sci 119:2246-57 (2006). Read more (PubMed: 16723731) »