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Anti-ERK1 (phospho T202 + Y204) antibody (ab24157)

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Overview

  • Product nameAnti-ERK1 (phospho T202 + Y204) antibodySee all ERK1 primary antibodies ...
  • Description
    Rabbit polyclonal to ERK1 (phospho T202 + Y204)
  • Specificityab24157 reacts with Phospho-Thr202/Thr204 ERK/MAPK protein.
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse, Human, Xenopus laevis
  • Immunogen

    Phosphopeptide corresponding to amino acid residues surrounding Phospho-Thr202 and Phospho-Tyr204 of ERK/MAPK(Rat).

  • Positive controlrat brain lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab24157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 43 kDa).

Target

  • FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications    Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-204.

    • Target information above from: UniProt accession P27361 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      ERK 1 antibodyERK antibodyERK-1 antibody
      ERK1 antibodyERT 2 antibodyERT2 antibodyExtracellular Signal Regulated Kinase 1 antibodyExtracellular signal related kinase 1 antibodyExtracellular signal-regulated kinase 1 antibodyHGNC6877 antibodyHS44KDAP antibodyHUMKER1A antibodyInsulin Stimulated MAP2 Kinase antibodyInsulin-stimulated MAP2 kinase antibodyMAP kinase 1 antibodyMAP kinase 3 antibodyMAP Kinase antibodyMAP kinase isoform p44 antibodyMAPK 1 antibodyMAPK 3 antibodyMAPK antibodyMAPK1 antibodyMapk3 antibodyMGC20180 antibodyMicrotubule Associated Protein 2 Kinase antibodyMicrotubule-associated protein 2 kinase antibodyMitogen Activated Protein Kinase 3 antibodyMitogen-activated protein kinase 1 antibodyMitogen-activated protein kinase 3 antibodyMK03_HUMAN antibodyMNK1 antibodyOTTHUMP00000174538 antibodyOTTHUMP00000174541 antibodyp44 ERK1 antibodyp44 MAPK antibodyp44-ERK1 antibodyp44-MAPK antibodyP44ERK1 antibodyP44MAPK antibodyPRKM 3 antibodyPRKM3 antibodyProtein Kinase Mitogen Activated 3 antibody
    see all

Anti-ERK1 (phospho T202 + Y204) antibody images

References for Anti-ERK1 (phospho T202 + Y204) antibody (ab24157)

ab24157 has not yet been referenced specifically in any publications.

Publishing research using ab24157 ? Please let us know so that we can cite the reference in this datasheet

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What is the concentration of this antibody?

Thank you for your enquiry. The concentration of this antibody is 0.1- 0.2 mg/ml. Please contact us again if you have any additional questions.

I checked the results another post-doc got on K562 phosphorylation status for Erk, and it was highly phosphorylated (compared to K562 stably expressing our gene of interest). This has been done with the Cell Signaling Technology rabbit antibody. As we ha...

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Thank you for your email. We have not had any complaints regarding this antibody. I would suggest increasing the concentration to 1:500 overnight at 4C. Also, please make sure that the protein transferred and that your secondary antibody is working prop...

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BATCH NUMBER 134245 ORDER NUMBER ? DESCRIPTION OF THE PROBLEM No signal for the phospho Erk antibody, whereas the Erk Ab (ab17942) worked really well on the identical lysates; I used the same anti-rabbit mixture as second antibody, so I...

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Thank you for your enquiry. Just because there is Erk present in your samples does not mean that there is also dually phosphorylated (or active) Erk present as well. There are two possibilities here: either there is no pErk present in these samples, or ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"