Anti-ERK1 (phospho T202) antibody (ab194776)
Key features and details
- Rabbit polyclonal to ERK1 (phospho T202)
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ERK1 (phospho T202) antibody
See all ERK1 primary antibodies -
Description
Rabbit polyclonal to ERK1 (phospho T202) -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human ERK1 (phospho T202). Phospho specific peptide corresponding to residues surrounding Threonine 202 of Human ERK1.
Database link: P27361 -
Positive control
- 293 cell extract, treated with PMA; Human breast carcinoma tissue; HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab194776 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/100.
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WB |
1/500 - 1/2000. Predicted molecular weight: 43 kDa.
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ICC/IF |
1/100 - 1/200.
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Notes |
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IHC-P
1/50 - 1/100. |
WB
1/500 - 1/2000. Predicted molecular weight: 43 kDa. |
ICC/IF
1/100 - 1/200. |
Target
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Function
Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4). -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
Domain
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
Post-translational
modificationsDually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-204. - Information by UniProt
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Database links
- Entrez Gene: 5595 Human
- Omim: 601795 Human
- SwissProt: P27361 Human
- Unigene: 861 Human
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Alternative names
- ERK 1 antibody
- ERK-1 antibody
- ERK1 antibody
see all
Images
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All lanes : Anti-ERK1 (phospho T202) antibody (ab194776) at 1/500 dilution
Lane 1 : 293 cell extract, untreated
Lane 2 : 293 cell extract, treated with PMA
Predicted band size: 43 kDa -
Immunofluorescent analysis of methanol-fixed HeLa cells labeling ERK1 (phospho T202) with ab194776 at 1/100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling ERK1 (phospho T202) with ab194776 at 1/50 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab194776 has been referenced in 4 publications.
- Lu C et al. Tumor necrosis factor receptor type 1-associated death domain (TRADD) regulates epithelial-mesenchymal transition (EMT), M1/M2 macrophage polarization and ectopic endometrial cysts formation in endometriosis. Ann Transl Med 9:148 (2021). PubMed: 33569450
- Sun H & Sun Y Lidocaine inhibits proliferation and metastasis of lung cancer cell via regulation of miR-539/EGFR axis. Artif Cells Nanomed Biotechnol 47:2866-2874 (2019). PubMed: 31299862
- Wang Y et al. Angiotensin II receptor blocker LCZ696 attenuates cardiac remodeling through the inhibition of the ERK signaling pathway in mice with pregnancy-associated cardiomyopathy. Cell Biosci 9:86 (2019). PubMed: 31649814
- Shen T et al. Circ_0026344 restrains metastasis of human colorectal cancer cells via miR-183. Artif Cells Nanomed Biotechnol 47:4038-4045 (2019). PubMed: 31608699