Overview

  • Product nameAnti-ERK2 antibody [1A8]See all ERK2 primary antibodies ...
  • Description
    Mouse monoclonal [1A8] to ERK2
  • Tested applicationsWB, ELISA more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    His-tagged recombinant human ERK2 protein purified from E.coli.

  • Positive control
    • 293T, HepG2, NIH 3T3 and C6 cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab70532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
ELISA Use at an assay dependent dilution.

Target

  • FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
    Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications
    Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
  • Cellular localizationNucleus.
  • Target information above from: UniProt accession P28482 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • ERK 2 antibody
    • ERK antibody
    • ERK-2 antibody
    • ERT 1 antibody
    • ERT1 antibody
    • Extracellular Signal Regulated Kinase 2 antibody
    • Extracellular signal-regulated kinase 2 antibody
    • MAP kinase 1 antibody
    • MAP kinase 2 antibody
    • MAP kinase 2 antibody
    • MAP kinase isoform p42 antibody
    • MAPK 1 antibody
    • MAPK 2 antibody
    • MAPK 2 antibody
    • MAPK1 antibody
    • MAPK2 antibody
    • Mitogen Activated Protein Kinase 1 antibody
    • Mitogen Activated Protein Kinase 2 antibody
    • Mitogen-activated protein kinase 1 antibody
    • Mitogen-activated protein kinase 2 antibody
    • MK01_HUMAN antibody
    • P38 antibody
    • P40 antibody
    • P41 antibody
    • P41MAPK antibody
    • p42 MAPK antibody
    • p42-MAPK antibody
    • P42MAPK antibody
    • PRKM 1 antibody
    • PRKM 2 antibody
    • PRKM1 antibody
    • PRKM2 antibody
    • Protein Tyrosine Kinase ERK2 antibody
    see all

Anti-ERK2 antibody [1A8] images

  • All lanes : Anti-ERK2 antibody [1A8] (ab70532) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : NIH 3T3 cell lysate
    Lane 4 : C6 cell lysate


    Predicted band size : 41 kDa
    Observed band size : 41 kDa
    Additional bands at : 83 kDa. We are unsure as to the identity of these extra bands.

References for Anti-ERK2 antibody [1A8] (ab70532)

ab70532 has not yet been referenced specifically in any publications.

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