Anti-ERK2 antibody [3F8] (ab96475)

Overview

• Product nameAnti-ERK2 antibody [3F8]See all ERK2 primary antibodies ...
• Description
  Mouse monoclonal [3F8] to ERK2
• Tested applicationsWB, ELISA, Flow Cyt more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  Purified recombinant fragment of Human ERK2 expressed in E. Coli.

• Positive controlJurkat, Hela, A431 and NIH3T3 cell lysate; Jurkat cells.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.03% Sodium Azide
  Constituents: Ascites
• Concentration information loading...
• PurityAscites
• Clonality Monoclonal
• Clone number3F8
• IsotypeIgG2b
• Research Areas
  • Stem Cells
  • Signaling Pathways
  • TGF beta
  • Cytoplasmic

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • MAPK Pathway

  • Neuroscience
  • Neurotransmission
  • Intracellular Signaling
  • Kinases

Applications

Target

• FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
  Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
• Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
  Contains 1 protein kinase domain.
• DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
• Post-translational
  modificationsDually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
• Cellular localizationNucleus.

Target information above from: UniProt accession P28482 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5594 Human
  • Entrez Gene: 26413 Mouse
  • Omim: 176948 Human
  • SwissProt: P28482 Human
  • SwissProt: P63085 Mouse
  • Unigene: 431850 Human
  • Unigene: 196581 Mouse

• Alternative names
  ERK 2 antibodyERK antibodyERK-2 antibody

  ERT1 antibodyExtracellular Signal Regulated Kinase 2 antibodyExtracellular signal-regulated kinase 2 antibodyMAP kinase 1 antibodyMAP kinase 2 antibodyMAP kinase 2 antibodyMAP kinase isoform p42 antibodyMAPK 1 antibodyMAPK 2 antibodyMAPK 2 antibodyMapk1 antibodyMAPK2 antibodyMitogen-activated protein kinase 1 antibodyMitogen-activated protein kinase 2 antibodyMK01_HUMAN antibodyP38 antibodyP40 antibodyP41 antibodyp42-MAPK antibodyP42MAPK antibodyPRKM1 antibodyPRKM2 antibody

  see all

Anti-ERK2 antibody [3F8] images

• 

  Western blot - ERK2 antibody [3F8] (ab96475)
  All lanes : Anti-ERK2 antibody [3F8] (ab96475) at 1/500 dilution
  
  Lane 1 : Jurkat cell lysate
  Lane 2 : Hela cell lysate
  Lane 3 : A431 cell lysate
  Lane 4 : NIH3T3 cell lysate
  
  
  Predicted band size : 41 kDa
  
• 

  Flow Cytometry - ERK2 antibody [3F8] (ab96475)
  ab96475 at 1/200 dilution staining ERK2 in Jurkat cells by Flow Cytometry (shown in green), negative control in purple.