Overview

  • Product nameAnti-ERK2 antibody - C-terminal
    See all ERK2 primary antibodies
  • Description
    Rabbit polyclonal to ERK2 - C-terminal
  • Tested applicationsELISA, IHC-P, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human ERK2 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • Positive control
    • UV-treated HL60 cell lysate and human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab72096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
Flow Cyt Use at an assay dependent dilution.

Target

  • FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
    Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications
    Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ERK 2 antibody
    • ERK antibody
    • ERK-2 antibody
    • ERT 1 antibody
    • ERT1 antibody
    • Extracellular Signal Regulated Kinase 2 antibody
    • Extracellular signal-regulated kinase 2 antibody
    • MAP kinase 1 antibody
    • MAP kinase 2 antibody
    • MAP kinase 2 antibody
    • MAP kinase isoform p42 antibody
    • MAPK 1 antibody
    • MAPK 2 antibody
    • MAPK 2 antibody
    • MAPK1 antibody
    • MAPK2 antibody
    • Mitogen Activated Protein Kinase 1 antibody
    • Mitogen Activated Protein Kinase 2 antibody
    • Mitogen-activated protein kinase 1 antibody
    • Mitogen-activated protein kinase 2 antibody
    • MK01_HUMAN antibody
    • P38 antibody
    • P40 antibody
    • P41 antibody
    • P41MAPK antibody
    • p42 MAPK antibody
    • p42-MAPK antibody
    • P42MAPK antibody
    • PRKM 1 antibody
    • PRKM 2 antibody
    • PRKM1 antibody
    • PRKM2 antibody
    • Protein Tyrosine Kinase ERK2 antibody
    see all

Anti-ERK2 antibody - C-terminal images

  • Anti-ERK2 antibody - C-terminal (ab72096) at 1/100 dilution + UV-treated HL60 cell lysate at 12.5 µg

    Secondary
    HRP-anti-rabbit

    Predicted band size : 41 kDa
    Observed band size : 41 kDa
    Additional bands at : 21 kDa,70 kDa. We are unsure as to the identity of these extra bands.
  • ab72096 at 1/50 dilution, staining ERK2 in human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.

References for Anti-ERK2 antibody - C-terminal (ab72096)

ab72096 has not yet been referenced specifically in any publications.

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