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Synthetic peptide corresponding to residues near the C terminal of human ERK2
Produced under U.S. Patent No. 5,675,063.
A trial size is available for this product.
Our Abpromise guarantee covers the use of ab32081 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Detects a band of approximately 42 kDa (predicted molecular weight: 41 kDa).|
|IHC-P||Use at an assay dependent concentration.|
|ICC||1/100 - 1/250.|
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab32081 overnight at 4°C. Antibody binding was detected using ab175781 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"