Anti-ERK2 antibody [E460] (ab32081)

Overview

• Product nameAnti-ERK2 antibody [E460]See all ERK2 primary antibodies ...
• Description
  Rabbit monoclonal [E460] to ERK2
• Specificityab32081 recognises ERK2
• Tested applicationsWB, IHC-P, ICC, Flow Cyt, IP more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic peptide corresponding to residues near the C terminal of human ERK2

• Epitopeab32081 reacts with an epitope located in the C terminal region of ERK2.
• Positive controlHuman breast carcinoma and A431 cell lysate
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• Concentration information loading...
• PurityProtein A purified
• Clonality Monoclonal
• Clone numberE460
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Signaling Pathways
  • TGF beta
  • Cytoplasmic

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • MAPK Pathway

  • Neuroscience
  • Neurotransmission
  • Intracellular Signaling
  • Kinases

Applications

Target

• FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
  Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
• Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
  Contains 1 protein kinase domain.
• DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
• Post-translational
  modificationsDually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
• Cellular localizationNucleus.

Target information above from: UniProt accession P28482 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5594 Human
  • Entrez Gene: 26413 Mouse
  • Entrez Gene: 116590 Rat
  • Omim: 176948 Human
  • SwissProt: P28482 Human
  • SwissProt: P63085 Mouse
  • SwissProt: P63086 Rat
  • Unigene: 431850 Human

  • Unigene: 196581 Mouse
  • Unigene: 34914 Rat

  see all

• Alternative names
  ERK 2 antibodyERK antibodyERK-2 antibody

  ERT 1 antibodyERT1 antibodyExtracellular Signal Regulated Kinase 2 antibodyExtracellular signal-regulated kinase 2 antibodyMAP kinase 1 antibodyMAP kinase 2 antibodyMAP kinase 2 antibodyMAP kinase isoform p42 antibodyMAPK 1 antibodyMAPK 2 antibodyMAPK 2 antibodyMAPK1 antibodyMAPK2 antibodyMitogen Activated Protein Kinase 1 antibodyMitogen Activated Protein Kinase 2 antibodyMitogen-activated protein kinase 1 antibodyMitogen-activated protein kinase 2 antibodyMK01_HUMAN antibodyP38 antibodyP40 antibodyP41 antibodyP41MAPK antibodyp42 MAPK antibodyp42-MAPK antibodyP42MAPK antibodyPRKM 1 antibodyPRKM 2 antibodyPRKM1 antibodyPRKM2 antibodyProtein Tyrosine Kinase ERK2 antibody

  see all

Anti-ERK2 antibody [E460] images

• 

  Western blot - ERK2 antibody [E460] (ab32081)
  Anti-ERK2 antibody [E460] (ab32081) at 1/2000 dilution + A431 cell lysate
  
  Predicted band size : 41 kDa
  Observed band size : 41 kDa
  
• 

  Immunohistochemistry (Paraffin-embedded sections) - ERK2 antibody [E460] (ab32081)
  Immunohistochemical analysis of ERK2 expression in paraffin embedded human breast carcinoma tissue section, using ab31081 at a dilution of 1/250.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK2 antibody [E460] (ab32081)Image from Emmanuel C et al., PLoS One. 2011 Mar 15;6(3):e17617. doi: 10.1371/journal.pone.0017617.; Fig 2.; March 15, 2011, PLoS ONE 6(3): e17617.
  Immunohistochemical analysis of Human fallopian tissue epithelium, staining ERK2 with ab32081 at 1/50 dilution. Samples were incubated with primary antibody for 1 hour at room temperature. Staining was detected using DAB.