Overview

  • Product nameAnti-ERK2 antibody [E460]
    See all ERK2 primary antibodies
  • Description
    Rabbit monoclonal [E460] to ERK2
  • Specificityab32081 recognises ERK2
  • Tested applicationsWB, IHC-P, ICC, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Recombinant Fragment
  • Immunogen

    Synthetic peptide corresponding to residues near the C terminal of human ERK2

  • Epitopeab32081 reacts with an epitope located in the C terminal region of ERK2.
  • Positive control
    • This antibody gave a positive signal within WB in the following whole cell lysates; HeLa; HEK293; MES; PC12 as well as ERK2 Recombinant protein. Human breast carcinoma and A431 cell lysate
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A 40 µl trial size is available to purchase for this antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab32081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 42 kDa (predicted molecular weight: 41 kDa).
IHC-P Use at an assay dependent concentration.
ICC 1/100 - 1/250.
Flow Cyt 1/1000.
IP 1/80.

Target

  • FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
    Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications
    Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ERK 2 antibody
    • ERK antibody
    • ERK-2 antibody
    • ERT 1 antibody
    • ERT1 antibody
    • Extracellular Signal Regulated Kinase 2 antibody
    • Extracellular signal-regulated kinase 2 antibody
    • MAP kinase 1 antibody
    • MAP kinase 2 antibody
    • MAP kinase isoform p42 antibody
    • MAPK 1 antibody
    • MAPK 2 antibody
    • Mapk1 antibody
    • MAPK2 antibody
    • Mitogen Activated Protein Kinase 1 antibody
    • Mitogen Activated Protein Kinase 2 antibody
    • Mitogen-activated protein kinase 1 antibody
    • Mitogen-activated protein kinase 2 antibody
    • MK01_HUMAN antibody
    • P38 antibody
    • P40 antibody
    • P41 antibody
    • P41MAPK antibody
    • p42 MAPK antibody
    • p42-MAPK antibody
    • P42MAPK antibody
    • PRKM 1 antibody
    • PRKM 2 antibody
    • PRKM1 antibody
    • PRKM2 antibody
    • Protein Tyrosine Kinase ERK2 antibody
    see all

Anti-ERK2 antibody [E460] images

  • All lanes : Anti-ERK2 antibody [E460] (ab32081) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate
    Lane 4 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 5 : Human ERK1 full length protein (ab43623)
    Lane 6 : Human ERK2 full length protein (ab43625)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

    Predicted band size : 41 kDa
    Observed band size : 41 kDa

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab32081 overnight at 4°C. Antibody binding was detected using ab175781 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

  • Anti-ERK2 antibody [E460] (ab32081) at 1/2000 dilution + A431 cell lysate

    Predicted band size : 41 kDa
    Observed band size : 41 kDa
  • Immunohistochemical analysis of ERK2 expression in paraffin embedded human breast carcinoma tissue section, using ab31081 at a dilution of 1/250.Immunohistochemical analysis of ERK2 expression in paraffin embedded human breast carcinoma tissue section, using ab31081 at a dilution of 1/250.
  • Immunohistochemical analysis of Human fallopian tissue epithelium, staining ERK2 with ab32081 at 1/50 dilution. Samples were incubated with primary antibody for 1 hour at room temperature. Staining was detected using DAB.
  • Overlay histogram showing HeLa cells stained with ab32081 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32081, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

References for Anti-ERK2 antibody [E460] (ab32081)

This product has been referenced in:
  • Chen M  et al. Targeting primitive chronic myeloid leukemia cells by effective inhibition of a new AHI-1-BCR-ABL-JAK2 complex. J Natl Cancer Inst 105:405-23 (2013). Read more (PubMed: 23446755) »
  • Emmanuel C  et al. Comparison of expression profiles in ovarian epithelium in vivo and ovarian cancer identifies novel candidate genes involved in disease pathogenesis. PLoS One 6:e17617 (2011). IHC-P ; Human . Read more (PubMed: 21423607) »

See all 3 Publications for this product

Product Wall

Application Flow Cytometry
Fixation Paraformaldehyde
Permeabilization Yes - 70% methanol
Sample Human Cell (HEK293)
Specification HEK293
Gating Strategy live cells gated
Preparation Cell harvesting/tissue preparation method: Cell dissociation buffer
Sample buffer: Enzyme free
Username

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Verified customer

Submitted Aug 04 2014

Application Western blot
Loading amount 50000 cells
Gel Running Conditions Reduced Denaturing (10%)
Sample Human Cell lysate - whole cell (HEK293)
Specification HEK293
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Aug 04 2014

I heard back from the lab that the ERK2 antibody is raised against a C-terminal sequence that shares 75% homology with ERK1.

Generally we say that anything with > 85% sequence homology would be expected to cross-react, so this is a bit low...

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