Overview

  • Product nameAnti-ERK2 antibody [E460]See all ERK2 primary antibodies ...
  • Description
    Rabbit monoclonal [E460] to ERK2
  • ConjugationAlexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Specificityab32081 recognises ERK2
  • Tested applicationsWB, IHC-P, ICC, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Recombinant Fragment
  • Immunogen

    Synthetic peptide corresponding to residues near the C terminal of human ERK2

  • Epitopeab32081 reacts with an epitope located in the C terminal region of ERK2.
  • Positive control
    • This antibody gave a positive signal within WB in the following whole cell lysates; HeLa; HEK293; MES; PC12 as well as ERK2 Recombinant protein. Human breast carcinoma and A431 cell lysate
  • General notes

    Produced under U.S. Patent No. 5,675,063.

    A trial size is available for this product.

Properties

Applications

Our Abpromise guarantee covers the use of ab32081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 42 kDa (predicted molecular weight: 41 kDa).
IHC-P Use at an assay dependent concentration.
ICC 1/100 - 1/250.
Flow Cyt 1/1000.
IP 1/80.

Target

  • FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
    Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications
    Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ERK 2 antibody
    • ERK antibody
    • ERK-2 antibody
    • ERT 1 antibody
    • ERT1 antibody
    • Extracellular Signal Regulated Kinase 2 antibody
    • Extracellular signal-regulated kinase 2 antibody
    • MAP kinase 1 antibody
    • MAP kinase 2 antibody
    • MAP kinase isoform p42 antibody
    • MAPK 1 antibody
    • MAPK 2 antibody
    • Mapk1 antibody
    • MAPK2 antibody
    • Mitogen Activated Protein Kinase 1 antibody
    • Mitogen Activated Protein Kinase 2 antibody
    • Mitogen-activated protein kinase 1 antibody
    • Mitogen-activated protein kinase 2 antibody
    • MK01_HUMAN antibody
    • P38 antibody
    • P40 antibody
    • P41 antibody
    • P41MAPK antibody
    • p42 MAPK antibody
    • p42-MAPK antibody
    • P42MAPK antibody
    • PRKM 1 antibody
    • PRKM 2 antibody
    • PRKM1 antibody
    • PRKM2 antibody
    • Protein Tyrosine Kinase ERK2 antibody
    see all

Anti-ERK2 antibody [E460] images

  • All lanes : Anti-ERK2 antibody [E460] (ab32081) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate
    Lane 4 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 5 : Human ERK1 full length protein (ab43623)
    Lane 6 : Human ERK2 full length protein (ab43625)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

    Predicted band size : 41 kDa
    Observed band size : 41 kDa

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab32081 overnight at 4°C. Antibody binding was detected using ab175781 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

  • Anti-ERK2 antibody [E460] (ab32081) at 1/2000 dilution + A431 cell lysate

    Predicted band size : 41 kDa
    Observed band size : 41 kDa
  • Immunohistochemical analysis of ERK2 expression in paraffin embedded human breast carcinoma tissue section, using ab31081 at a dilution of 1/250.Immunohistochemical analysis of ERK2 expression in paraffin embedded human breast carcinoma tissue section, using ab31081 at a dilution of 1/250.
  • Immunohistochemical analysis of Human fallopian tissue epithelium, staining ERK2 with ab32081 at 1/50 dilution. Samples were incubated with primary antibody for 1 hour at room temperature. Staining was detected using DAB.
  • Overlay histogram showing HeLa cells stained with ab32081 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32081, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

References for Anti-ERK2 antibody [E460] (ab32081)

This product has been referenced in:
  • Chen M  et al. Targeting primitive chronic myeloid leukemia cells by effective inhibition of a new AHI-1-BCR-ABL-JAK2 complex. J Natl Cancer Inst 105:405-23 (2013). Read more (PubMed: 23446755) »
  • Emmanuel C  et al. Comparison of expression profiles in ovarian epithelium in vivo and ovarian cancer identifies novel candidate genes involved in disease pathogenesis. PLoS One 6:e17617 (2011). IHC-P ; Human . Read more (PubMed: 21423607) »

See all 3 Publications for this product

Product Wall

I heard back from the lab that the ERK2 antibody is raised against a C-terminal sequence that shares 75% homology with ERK1.

Generally we say that anything with > 85% sequence homology would be expected to cross-react, so this is a bit low...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"