Anti-ERK2 antibody [E460] (ab32081)
- Product nameAnti-ERK2 antibody [E460]See all ERK2 primary antibodies ...
- DescriptionRabbit monoclonal [E460] to ERK2
- Specificityab32081 recognises ERK2
- Tested applicationsWB, IHC-P, ICC, Flow Cyt, IP more details
- Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to residues near the C terminal of human ERK2
- Epitopeab32081 reacts with an epitope located in the C terminal region of ERK2.
- Positive controlHuman breast carcinoma and A431 cell lysate
- General notesProduced under U.S. Patent No. 5,675,063.
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
- Concentration information loading...
- PurityProtein A purified
- Clonality Monoclonal
- Clone numberE460
- Research Areas
Our Abpromise guarantee covers the use of ab32081 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: 1/1000 - 1/2000. Detects a band of approximately 42 kDa (predicted molecular weight: 41 kDa).|
|IHC-P||IHC-P: Use at an assay dependent dilution.|
|ICC||ICC: 1/100 - 1/250.|
|Flow Cyt||Flow Cyt: 1/50.|
- FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
- Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.
- DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
modificationsDually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
- Cellular localizationNucleus.
- ERK 2 antibodyERK antibodyERK-2 antibody
- ERT 1 antibodyERT1 antibodyExtracellular Signal Regulated Kinase 2 antibodyExtracellular signal-regulated kinase 2 antibodyMAP kinase 1 antibodyMAP kinase 2 antibodyMAP kinase 2 antibodyMAP kinase isoform p42 antibodyMAPK 1 antibodyMAPK 2 antibodyMAPK 2 antibodyMAPK1 antibodyMAPK2 antibodyMitogen Activated Protein Kinase 1 antibodyMitogen Activated Protein Kinase 2 antibodyMitogen-activated protein kinase 1 antibodyMitogen-activated protein kinase 2 antibodyMK01_HUMAN antibodyP38 antibodyP40 antibodyP41 antibodyP41MAPK antibodyp42 MAPK antibodyp42-MAPK antibodyP42MAPK antibodyPRKM 1 antibodyPRKM 2 antibodyPRKM1 antibodyPRKM2 antibodyProtein Tyrosine Kinase ERK2 antibody
Anti-ERK2 antibody [E460] images
Anti-ERK2 antibody [E460] (ab32081) at 1/2000 dilution + A431 cell lysate
Predicted band size : 41 kDa
Observed band size : 41 kDa
Immunohistochemical analysis of ERK2 expression in paraffin embedded human breast carcinoma tissue section, using ab31081 at a dilution of 1/250.
Immunohistochemical analysis of Human fallopian tissue epithelium, staining ERK2 with ab32081 at 1/50 dilution. Samples were incubated with primary antibody for 1 hour at room temperature. Staining was detected using DAB.
References for Anti-ERK2 antibody [E460] (ab32081)
This product has been referenced in:
- Emmanuel C et al. Comparison of expression profiles in ovarian epithelium in vivo and ovarian cancer identifies novel candidate genes involved in disease pathogenesis. PLoS One 6:e17617 (2011). IHC-P ; Human . Read more (PubMed: 21423607) »
- Burkhard K & Shapiro P Use of inhibitors in the study of MAP kinases. Methods Mol Biol 661:107-22 (2010). Read more (PubMed: 20811979) »