Overview

  • Product nameAnti-ERK2 antibody
    See all ERK2 primary antibodies
  • Description
    Goat polyclonal to ERK2
  • Specificityab27505 recognises human ERK2 / MAPK1 protein.
  • Tested applicationsELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide:

    CA-AGPEMVRGQVF,

    corresponding to amino acids 9-19 of Human ERK2

  • EpitopeThis antibody is expected to recognise an epitope corresponding to a.a. 9-19 of both reported protein isoforms of human ERK2 / MAPK1 protein.
  • Positive control
    • Human cervix epitheloid carcinoma HeLa; Human hepatoblastoma HepG2 lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab27505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesELISA: 1/8000 - antibody detection limit.
    WB: Use at a concentration of 0.5-2.0 µg/ml. Predicted molecular weight: 41.4 kDa. Can be blocked with ERK2 peptide (9-19) (ab45662).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
      Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
    • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
      Contains 1 protein kinase domain.
    • DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
    • Post-translational
      modifications
      Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • ERK 2 antibody
      • ERK antibody
      • ERK-2 antibody
      • ERT 1 antibody
      • ERT1 antibody
      • Extracellular Signal Regulated Kinase 2 antibody
      • Extracellular signal-regulated kinase 2 antibody
      • MAP kinase 1 antibody
      • MAP kinase 2 antibody
      • MAP kinase 2 antibody
      • MAP kinase isoform p42 antibody
      • MAPK 1 antibody
      • MAPK 2 antibody
      • MAPK 2 antibody
      • MAPK1 antibody
      • MAPK2 antibody
      • Mitogen Activated Protein Kinase 1 antibody
      • Mitogen Activated Protein Kinase 2 antibody
      • Mitogen-activated protein kinase 1 antibody
      • Mitogen-activated protein kinase 2 antibody
      • MK01_HUMAN antibody
      • P38 antibody
      • P40 antibody
      • P41 antibody
      • P41MAPK antibody
      • p42 MAPK antibody
      • p42-MAPK antibody
      • P42MAPK antibody
      • P42MAPK antibody
      • PRKM 1 antibody
      • PRKM 2 antibody
      • PRKM1 antibody
      • PRKM2 antibody
      • Protein Tyrosine Kinase ERK2 antibody
      see all

    Anti-ERK2 antibody images

    References for Anti-ERK2 antibody (ab27505)

    ab27505 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us with your question about these antibodies. Neither ab27505 or ab14018 has been tested in IF or IHC, and they have only been tested against the denatured protein in Western blot or ELISA. It is possible that one or bo...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"