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Overview

  • Product nameAnti-ERK2 antibodySee all ERK2 primary antibodies ...
  • Description
    Goat polyclonal to ERK2
  • Specificityab27505 recognises human ERK2 / MAPK1 protein.
  • Tested applicationsELISA, WB more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide: CA-AGPEMVRGQVF, corresponding to amino acids 9-19 of Human ERK2

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • EpitopeThis antibody is expected to recognise an epitope corresponding to a.a. 9-19 of both reported protein isoforms of human ERK2 / MAPK1 protein.
  • Positive controlHuman cervix epitheloid carcinoma HeLa; Human hepatoblastoma HepG2 lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab27505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA
WB
  • Application notesELISA: 1/8000 - antibody detection limit.
    WB: Use at a concentration of 0.5-2.0 µg/ml. Predicted molecular weight: 41.4 kDa. Can be blocked with ERK2 peptide (9-19) (ab45662).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
      Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
    • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
      Contains 1 protein kinase domain.
    • DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
    • Post-translational
      modifications      Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
    • Cellular localizationNucleus.

      • Target information above from: UniProt accession P28482 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      see all
    • Alternative names
        ERK 2 antibodyERK antibodyERK-2 antibody
        ERT 1 antibodyERT1 antibodyExtracellular Signal Regulated Kinase 2 antibodyExtracellular signal-regulated kinase 2 antibodyMAP kinase 1 antibodyMAP kinase 2 antibodyMAP kinase 2 antibodyMAP kinase isoform p42 antibodyMAPK 1 antibodyMAPK 2 antibodyMAPK 2 antibodyMAPK1 antibodyMAPK2 antibodyMitogen Activated Protein Kinase 1 antibodyMitogen Activated Protein Kinase 2 antibodyMitogen-activated protein kinase 1 antibodyMitogen-activated protein kinase 2 antibodyMK01_HUMAN antibodyP38 antibodyP40 antibodyP41 antibodyP41MAPK antibodyp42 MAPK antibodyp42-MAPK antibodyP42MAPK antibodyP42MAPK antibodyPRKM 1 antibodyPRKM 2 antibodyPRKM1 antibodyPRKM2 antibodyProtein Tyrosine Kinase ERK2 antibody
      see all

    Anti-ERK2 antibody images

    References for Anti-ERK2 antibody (ab27505)

    ab27505 has not yet been referenced specifically in any publications.

    Publishing research using ab27505 ? Please let us know so that we can cite the reference in this datasheet

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    Hi,   I am thinking of purchasing these two antibodies;   -Goat Anti-ERK2 antibody (ab27505) -Chicken Anti-ERK2 antibody (ab14018) My question is if they have been tested and evaluated for use in any kind of Immunofluorescence and/...

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    Thank you for contacting us with your question about these antibodies. Neither ab27505 or ab14018 has been tested in IF or IHC, and they have only been tested against the denatured protein in Western blot or ELISA. It is possible that one or both ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"