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Synthetic peptide (Rat) (C terminal).
Our Abpromise guarantee covers the use of ab7948 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||1/300. ABC amplification recommended.|
|WB||Use at an assay dependent concentration. Can be blocked with ERK2 peptide (ab7992). (see Abreview).|
|IP||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
Lanes 1, 3 and 5: Wild-type HAP1 cell lysate (20 µg)
Lanes 2, 4 and 6: ERK2 knockout HAP1 cell lysate (20 µg)
Lanes 1 and 2: Green signal from target - ab7948 observed at 44 kDa
Lanes 3 and 4: Red signal from loading control - ab8226 observed at 42 kDa
Lanes 5 and 6: Merged (red and green) signal
ab7948 was shown to specifically react with ERK2 when ERK2 knockout samples were used. Wild-type and ERK2 knockout samples were subjected to SDS-PAGE. ab7948 and ab8226 (loading control to beta actin) were diluted at 1/2000 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
Immunofluorescence detection of ERK-2 antibody (ab7498) at a dilution of 1/300 (1 day incubation at RT). Cytoplasmic staining observed in the dorsal horn of the rat spinal cord (40x objective). Immunofluorescence staining performed on slide using PFA/picric acid perfusion-fixed tissue (30
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