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Synthetic peptide (Rat) (C terminal).
Our Abpromise guarantee covers the use of ab7948 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||1/300. ABC amplification recommended.|
|WB||Use at an assay dependent concentration. Can be blocked with ERK2 peptide (ab7992). (see Abreview).|
|IP||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
Immunofluorescence detection of ERK-2 antibody (ab7498) at a dilution of 1/300 (1 day incubation at RT). Cytoplasmic staining observed in the dorsal horn of the rat spinal cord (40x objective). Immunofluorescence staining performed on slide using PFA/picric acid perfusion-fixed tissue (30
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