Anti-ERK2 antibody (ab7948)
- Product nameAnti-ERK2 antibodySee all ERK2 primary antibodies ...
- DescriptionRabbit polyclonal to ERK2
- SpecificityThis antibody reacts with ERK 2 p42 and to a lesser extent ERK 1 p44.
- Tested applicationsIHC-FoFr, WB, IP, IHC-P more details
- Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (Rat) (C terminal).
- Positive controlHeLa, NIH/3T3, A-431 and HL-60
- Storage instructionsStore at +4°C. Do not freeze.
- Storage bufferPBS with 0.1% sodium azide
- Concentration information loading...
- PurityIgG fraction
- Clonality Polyclonal
- Research Areas
Our Abpromise guarantee covers the use of ab7948 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||IHC-FoFr: 1/300. ABC amplification recommended.|
|WB||WB: Use at an assay dependent concentration. Can be blocked with ERK2 (C-term) peptide (rat) (ab7992). (see Abreview).|
|IP||IP: Use at an assay dependent concentration.|
|IHC-P||IHC-P: Use at an assay dependent concentration.|
- FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
- Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.
- DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
modificationsDually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
- Cellular localizationNucleus.
- ERK 2 antibodyERK antibodyERK-2 antibody
- ERT 1 antibodyERT1 antibodyExtracellular Signal Regulated Kinase 2 antibodyExtracellular signal-regulated kinase 2 antibodyMAP kinase 1 antibodyMAP kinase 2 antibodyMAP kinase 2 antibodyMAP kinase isoform p42 antibodyMAPK 1 antibodyMAPK 2 antibodyMAPK 2 antibodyMAPK1 antibodyMAPK2 antibodyMitogen Activated Protein Kinase 1 antibodyMitogen Activated Protein Kinase 2 antibodyMitogen-activated protein kinase 1 antibodyMitogen-activated protein kinase 2 antibodyMK01_HUMAN antibodyP38 antibodyP40 antibodyP41 antibodyP41MAPK antibodyp42 MAPK antibodyp42-MAPK antibodyP42MAPK antibodyP42MAPK antibodyPRKM 1 antibodyPRKM 2 antibodyPRKM1 antibodyPRKM2 antibodyProtein Tyrosine Kinase ERK2 antibody
Anti-ERK2 antibody images
ERK-2 antibody (ab7498) at a dilution of 1/1000 (2 X o/n incubation at RT; ABC amplification). Nuclear staining observed in lamina layers 1 and 2 of the spinal cord [A] 40x objective, [B] 20x objective. IHC staining performed on PFA perfusion-fixed, free floating 30
m saggital spinal cord sections. Find detailed information regarding the protocol in the Specific Protocol link. µ
Immunofluorescence detection of ERK-2 antibody (ab7498) at a dilution of 1/300 (1 day incubation at RT). Cytoplasmic staining observed in the dorsal horn of the rat spinal cord (40x objective). Immunofluorescence staining performed on slide using PFA/picric acid perfusion-fixed tissue (30
µm saggital spinal cord sections).
ab7948 staining ERK2 in A431 whole cell lysate (lane A), HeLa whole cell lysate (lane B), HL-60 whole cell lysate (lane C) and NIH / 3T3 whole cell lysate (lane D).
References for Anti-ERK2 antibody (ab7948)
This product has been referenced in:
- Fleming IN et al. Synergistic inhibition of ErbB signaling by combined treatment with seliciclib and ErbB-targeting agents. Clin Cancer Res 14:4326-35 (2008). WB ; Human . Read more (PubMed: 18594016) »