Overview

  • Product nameAnti-ERK2 antibodySee all ERK2 primary antibodies ...
  • Description
    Rabbit polyclonal to ERK2
  • SpecificityThis antibody reacts with ERK 2 p42 and to a lesser extent ERK 1 p44.
  • Tested applicationsIHC-FoFr, WB, IP, IHC-P more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Rat) (C terminal).

  • Positive control
    • HeLa, NIH/3T3, A-431 and HL-60

Properties

Applications

Our Abpromise guarantee covers the use of ab7948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-FoFr 1/300. ABC amplification recommended.
WB Use at an assay dependent concentration. Can be blocked with ERK2 peptide (ab7992). (see Abreview).
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
    Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications
    Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
  • Cellular localizationNucleus.
  • Target information above from: UniProt accession P28482 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • ERK 2 antibody
    • ERK antibody
    • ERK-2 antibody
    • ERT 1 antibody
    • ERT1 antibody
    • Extracellular Signal Regulated Kinase 2 antibody
    • Extracellular signal-regulated kinase 2 antibody
    • MAP kinase 1 antibody
    • MAP kinase 2 antibody
    • MAP kinase 2 antibody
    • MAP kinase isoform p42 antibody
    • MAPK 1 antibody
    • MAPK 2 antibody
    • MAPK 2 antibody
    • MAPK1 antibody
    • MAPK2 antibody
    • Mitogen Activated Protein Kinase 1 antibody
    • Mitogen Activated Protein Kinase 2 antibody
    • Mitogen-activated protein kinase 1 antibody
    • Mitogen-activated protein kinase 2 antibody
    • MK01_HUMAN antibody
    • P38 antibody
    • P40 antibody
    • P41 antibody
    • P41MAPK antibody
    • p42 MAPK antibody
    • p42-MAPK antibody
    • P42MAPK antibody
    • P42MAPK antibody
    • PRKM 1 antibody
    • PRKM 2 antibody
    • PRKM1 antibody
    • PRKM2 antibody
    • Protein Tyrosine Kinase ERK2 antibody
    see all

Anti-ERK2 antibody images


  • ERK-2 antibody (ab7498) at a dilution of 1/1000 (2 X o/n incubation at RT; ABC amplification). Nuclear staining observed in lamina layers 1 and 2 of the spinal cord [A] 40x objective, [B] 20x objective. IHC staining performed on PFA perfusion-fixed, free floating 30µm saggital spinal cord sections. Find detailed information regarding the protocol in the Specific Protocol link.
  • Immunofluorescence detection of ERK-2 antibody (ab7498) at a dilution of 1/300 (1 day incubation at RT). Cytoplasmic staining observed in the dorsal horn of the rat spinal cord (40x objective). Immunofluorescence staining performed on slide using PFA/picric acid perfusion-fixed tissue (30µm saggital spinal cord sections).

  • ab7948 staining ERK2 in A431 whole cell lysate (lane A), HeLa whole cell lysate (lane B), HL-60 whole cell lysate (lane C) and NIH / 3T3 whole cell lysate (lane D).

References for Anti-ERK2 antibody (ab7948)

This product has been referenced in:
  • Fleming IN  et al. Synergistic inhibition of ErbB signaling by combined treatment with seliciclib and ErbB-targeting agents. Clin Cancer Res 14:4326-35 (2008). WB ; Human . Read more (PubMed: 18594016) »

See 1 Publication for this product

Product Wall

Thank you for contacting Abcam.

You are correct in that ERK2 is p42 and that ERK1 isp44. What the datasheet should have said is that the antibody "reacts with ERK2 p42 and to a lesser extent ERK1 p44".

I am sorry about the...

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Thank you for calling today. As we discussed over the phone, I believe that the main reason for non-specific staining is the dilution of the primary antibody. The rest of your protocol looks fine to me. We have data suggesting that 1:1000 is...

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Application Western blot
Sample Mouse Tissue lysate - whole (retina)
Loading amount 30 µg
Specification retina
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Mar 21 2006

Thank you for your enquiry. I am able to provide you with the following information; for some of the antibodies that you are interested in, immunogen information is considered to be proprietary. Ab7948: The sequence is as follows: IFEETARFQPGYRS ...

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