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Overview

  • Product nameAnti-ERK2 antibodySee all ERK2 primary antibodies ...
  • Description
    Rabbit polyclonal to ERK2
  • SpecificityThis antibody reacts with ERK 2 p42 and to a lesser extent ERK 1 p44.
  • Tested applicationsIHC-FoFr, WB, IP, IHC-P more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Rat) (C terminal).

  • Positive controlHeLa, NIH/3T3, A-431 and HL-60

Properties

Applications

Our Abpromise guarantee covers the use of ab7948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-FoFr IHC-FoFr: 1/300. ABC amplification recommended.
WB WB: Use at an assay dependent concentration. Can be blocked with ERK2 (C-term) peptide (rat) (ab7992). (see Abreview).
IP IP: Use at an assay dependent concentration.
IHC-P IHC-P: Use at an assay dependent concentration.

Target

  • FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
    Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications    Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
  • Cellular localizationNucleus.

    • Target information above from: UniProt accession P28482 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      ERK 2 antibodyERK antibodyERK-2 antibody
      ERT 1 antibodyERT1 antibodyExtracellular Signal Regulated Kinase 2 antibodyExtracellular signal-regulated kinase 2 antibodyMAP kinase 1 antibodyMAP kinase 2 antibodyMAP kinase 2 antibodyMAP kinase isoform p42 antibodyMAPK 1 antibodyMAPK 2 antibodyMAPK 2 antibodyMAPK1 antibodyMAPK2 antibodyMitogen Activated Protein Kinase 1 antibodyMitogen Activated Protein Kinase 2 antibodyMitogen-activated protein kinase 1 antibodyMitogen-activated protein kinase 2 antibodyMK01_HUMAN antibodyP38 antibodyP40 antibodyP41 antibodyP41MAPK antibodyp42 MAPK antibodyp42-MAPK antibodyP42MAPK antibodyP42MAPK antibodyPRKM 1 antibodyPRKM 2 antibodyPRKM1 antibodyPRKM2 antibodyProtein Tyrosine Kinase ERK2 antibody
    see all

Anti-ERK2 antibody images

  • Immunohistochemistry (PFA perfusion fixed frozen sections) - ERK2 antibody (ab7948)
    Immunohistochemistry (PFA perfusion fixed frozen sections) - ERK2 antibody (ab7948)Image courtesy of Dr S Pezet, CARD institute, KCL, London, UK

    ERK-2 antibody (ab7498) at a dilution of 1/1000 (2 X o/n incubation at RT; ABC amplification). Nuclear staining observed in lamina layers 1 and 2 of the spinal cord [A] 40x objective, [B] 20x objective. IHC staining performed on PFA perfusion-fixed, free floating 30µm saggital spinal cord sections. Find detailed information regarding the protocol in the Specific Protocol link.
  • Immunocytochemistry/ Immunofluorescence - ERK2 antibody (ab7948)
    Immunocytochemistry/ Immunofluorescence - ERK2 antibody (ab7948)Image courtesy of Dr S Pezet, CARD institute, KCL, London, UK

    Immunofluorescence detection of ERK-2 antibody (ab7498) at a dilution of 1/300 (1 day incubation at RT). Cytoplasmic staining observed in the dorsal horn of the rat spinal cord (40x objective). Immunofluorescence staining performed on slide using PFA/picric acid perfusion-fixed tissue (30µm saggital spinal cord sections).

  • Western blot - Anti-ERK2 antibody (ab7948)
    Western blot - Anti-ERK2 antibody (ab7948)
    ab7948 staining ERK2 in A431 whole cell lysate (lane A), HeLa whole cell lysate (lane B), HL-60 whole cell lysate (lane C) and NIH / 3T3 whole cell lysate (lane D).

References for Anti-ERK2 antibody (ab7948)

This product has been referenced in:
  • Fleming IN  et al. Synergistic inhibition of ErbB signaling by combined treatment with seliciclib and ErbB-targeting agents. Clin Cancer Res 14:4326-35 (2008). WB ; Human . Read more (PubMed: 18594016) »

See 1 Publication for this product

Publishing research using ab7948 ? Please let us know so that we can cite the reference in this datasheet

Product Wall

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Displaying 1 - 4 of 4 results for Abreviews and Q&A

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I ordered one ERK2 antibody from Abcam (AB7948). From the product data sheet, it mentioned that ' this antibody reacts with ERK2 p44 and to a lesser extent ERK1 p42'. However, actually ERK2 is p42 while ERK1 is p44. Are you sure I got the right antibody?

Thank you for contacting Abcam.

You are correct in that ERK2 is p42 and that ERK1 isp44. What the datasheet should have said is that the antibody "reacts with ERK2 p42 and to a lesser extent ERK1 p44".

I am sorry about the co...

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Customer is performing IHC-P on mouse skin and getting non-specific staining. Antigen retrieval: Citrate buffer for 10 minutes Block: 10% normal goat serum, 1% BSA for 1 hr RT 1ary antibody: 1:50 overnight for 4C or 30 minutes RT 2ary: G...

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Thank you for calling today. As we discussed over the phone, I believe that the main reason for non-specific staining is the dilution of the primary antibody. The rest of your protocol looks fine to me. We have data suggesting that 1:1000 is an...

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Western blot abreview for Anti-ERK2 antibody

 Excellent
Abreviews
Application Western blot
Sample Mouse Tissue lysate - whole (retina)
Loading amount 30 µg
Specification retina
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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Verified customer

Submitted Mar 21 2006

can you provide immunogen sequences of the following antibodies? ab 2312 ab 5612 ab 5613 ab 4756 ab 4757 ab 9363 ab 7948 ab 4819

Thank you for your enquiry. I am able to provide you with the following information; for some of the antibodies that you are interested in, immunogen information is considered to be proprietary. Ab7948: The sequence is as follows: IFEETARFQPGYRS ...

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