Anti-ERLIN1 antibody (ab229261)
Key features and details
- Rabbit polyclonal to ERLIN1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-ERLIN1 antibody -
Description
Rabbit polyclonal to ERLIN1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Pig, Rhesus monkey -
Immunogen
Recombinant fragment within Human ERLIN1 (internal sequence). The exact sequence is proprietary.
Database link: O75477 -
Positive control
- IHC-P: Human hepatoma tissue. C2C12 and RT2 xenograft. WB: HepG2 whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229261 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 39 kDa.
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IHC-P |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 39 kDa. |
IHC-P
1/100 - 1/1000. |
Target
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Function
Component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs). -
Tissue specificity
Expressed in heart, placenta, liver, kidney, pancreas, prostate, testis, ovary and small intestine. -
Sequence similarities
Belongs to the band 7/mec-2 family. -
Cellular localization
Endoplasmic reticulum membrane. Associated with lipid raft-like domains of the endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 617074 Cow
- Entrez Gene: 10613 Human
- Entrez Gene: 226144 Mouse
- Entrez Gene: 100154360 Pig
- Entrez Gene: 293939 Rat
- Entrez Gene: 709596 Rhesus monkey
- Omim: 611604 Human
- SwissProt: O75477 Human
see all -
Alternative names
- Band 7 23-211 Keo4 (Interim) similar to C.elegans protein antibody
- C10orf69 antibody
- chromosome 10 open reading frame 69 antibody
see all
Images
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Anti-ERLIN1 antibody (ab229261) at 1/1000 dilution + HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 39 kDa10% SDS-PAGE
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Paraffin-embedded human hepatoma tissue stained for ERLIN1 with ab229261 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded RT2 xenograft stained for ERLIN1 with ab229261 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded C2C12 xenograft stained for ERLIN1 with ab229261 at 1/500 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab229261 has not yet been referenced specifically in any publications.