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Anti-ERM / Etv5 antibody (ab50393)

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Overview

Product name

Anti-ERM / Etv5 antibody
See all ERM / Etv5 products (5) ...

Description

Rabbit polyclonal to ERM / Etv5

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat, Cow, Dog

Immunogen

Synthetic peptide: The exact immunogen was derived from the following sequence: AQVPDDEQFV PDFQSDNLVL HAPPPTKIKR ELHSPSSELS SCSHEQALGA (Human) The exact immunogen is proprietary information.

AQVPDDEQFV PDFQSDNLVL HAPPPTKIKR ELHSPSSELS SCSHEQALGA

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

293T cell lysate

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 2% Sucrose, PBS

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Stem Cells >> Cancer Stem Cells >> Intracellular
Epigenetics and Nuclear Signaling >> Transcription >> Other factors

  • Western blot - ERM / Etv5 antibody (ab50393)Western blot - ERM / Etv5 antibody (ab50393) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab50393 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

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    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 1 µg/mlDetects a band of approximately 50 kDa (predicted molecular weight: 58 kDa).

Target

Function

Binds to DNA sequences containing the consensus nucleotide core sequence GGAA.

Tissue specificity

Ubiquitous.

Sequence similarities

Belongs to the ETS family.
Contains 1 ETS DNA-binding domain.

Cellular localization

Nucleus.

Target information above from: UniProt accessionP41161 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • ERM antibody
  • Ets related protein ERM antibody
  • ETS translocation variant 5 antibody
  • Ets variant gene 5 antibody
  • Ets-related protein ERM antibody
  • ETV5 antibody
  • ETV5_HUMAN antibody
see all

Database links

see all

Anti-ERM / Etv5 antibody images:

  Western blot - ERM / Etv5 antibody (ab50393)

Western blot - ERM / Etv5 antibody (ab50393)

Lane 1 : Marker
Lane 2 : Anti-ERM / Etv5 antibody (ab50393) at 1 µg/ml

Lane 1 : As above
Lane 2 : 293T cell lysate at 10 µg

Secondary
Lane 1 : HRP conjugated anti-Rabbit IgG at 1/50000-1/100000 dilution
Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000-1/100000 dilution


Predicted band size : 58 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)


Gel concentration 12%

References for Anti-ERM / Etv5 antibody (ab50393)

ab50393 has not yet been referenced specifically in any publications.

Publishing research using ab50393? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"