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Products:Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Overview
Product name
Anti-ERM / Etv5 antibody
See all ERM / Etv5 products (5) ...
Description
Rabbit polyclonal to ERM / Etv5
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Dog
Immunogen
A synthetic peptide corresponding to a region of human ERM / Etv5
Positive control
ERM / Etv5 transfected 293T cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Cancer Stem Cells >> Intracellular
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
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Western blot - ERM / Etv5 antibody (ab75170)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab75170 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
ELISA: Use at an assay dependent dilution. Titre using peptide based assay: 1/62500.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Binds to DNA sequences containing the consensus nucleotide core sequence GGAA.
Tissue specificity
Ubiquitous.
Sequence similarities
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP41161
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ERM antibody
- Ets related protein ERM antibody
- ETS translocation variant 5 antibody
see all
Database links
- Entrez Gene: 2119 Human
- Entrez Gene: 104156 Mouse
- Entrez Gene: 303828 Rat
- Omim: 601600 Human
- SwissProt: P41161 Human
- SwissProt: Q6IBN5 Human
- SwissProt: Q8BJR1 Mouse
- SwissProt: Q9CXC9 Mouse
see all
Anti-ERM / Etv5 antibody images:
Western blot - ERM / Etv5 antibody (ab75170)
Anti-ERM / Etv5 antibody (ab75170) at 1 µg/ml (in 5% skim milk / PBS buffer) + Transfected 293T cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 58 kDa
Observed band size : 58 kDa
Gel concentration: 12%
References for Anti-ERM / Etv5 antibody (ab75170)
ab75170 has not yet been referenced specifically in any publications.
Publishing research using ab75170? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"