Recombinant Anti-ERp18 antibody [EPR9025] (ab137035)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9025] to ERp18
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ERp18 antibody [EPR9025]
See all ERp18 primary antibodies -
Description
Rabbit monoclonal [EPR9025] to ERp18 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HepG2, Y79 or Human fetal kidney lysates; 293T cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9025 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137035 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/10000 - 1/50000. Predicted molecular weight: 19 kDa.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/10000 - 1/50000. Predicted molecular weight: 19 kDa. |
Target
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Function
Possesses significant protein thiol-disulfide oxidase activity. -
Tissue specificity
Widely expressed. -
Sequence similarities
Contains 1 thioredoxin domain. -
Cellular localization
Endoplasmic reticulum lumen. - Information by UniProt
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Database links
- Entrez Gene: 51060 Human
- Omim: 609448 Human
- SwissProt: O95881 Human
- Unigene: 476033 Human
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Alternative names
- AG1 antibody
- AGR1 antibody
- anterior gradient homolog 1 antibody
see all
Images
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All lanes : Anti-ERp18 antibody [EPR9025] (ab137035) at 1/10000 dilution
Lane 1 : 293T lysate
Lane 2 : HepG2 lysate
Lane 3 : Y-79 cell lysate
Lane 4 : Human fetal kidney lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP conjugated Goat anti Rabbit IgG at 1/2000 dilution
Predicted band size: 19 kDa -
Intracellular Flow Cytometry analysis of permeabilized 293T cells labelling ERp18 with ab137035 at 1/10 dilution (Red) or a Rabbit IgG (negative control) (Green).
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab137035 has not yet been referenced specifically in any publications.