Overview

  • Product nameAnti-ERp72 antibodySee all ERp72 primary antibodies ...
  • Description
    Mouse polyclonal to ERp72
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Vector coding for a partial recombinant fusion protein, corresponding to amino acids 520-620 of Human PDIR [Swiss Prot P13667]. Tarte sequence used to make antibody: PKNNKGPVKV VVGKTFDSIV MDPKKDVLIE FYAPWCGHCK QLEPVYNSLA KKYKGQKGLV IAKMDATAND VPSDRYKVEG FPTIYFAPSG DKKNPVKFEG.

  • General notes


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, Whole serum.
  • PurityWhole antiserum
  • Primary antibody notes This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab52841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
  • Application notesWB: 1/1000. Predicted molecular weight: 73 kDa.

    This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Sequence similaritiesBelongs to the protein disulfide isomerase family.
      Contains 3 thioredoxin domains.
    • Cellular localizationEndoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Target information above from: UniProt accession P13667 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • Calcium binding protein intestinal related antibody
      • Endoplasmic reticulum resident protein 70 antibody
      • Endoplasmic reticulum resident protein 72 antibody
      • ER protein 70 antibody
      • ER protein 72 antibody
      • ERP 70 antibody
      • ERp 72 antibody
      • ERp-72 antibody
      • ERP70 antibody
      • ERp72 antibody
      • PDIA 4 antibody
      • PDIA4 antibody
      • PDIA4_HUMAN antibody
      • PDIR antibody
      • Protein disulfide isomerase A4 antibody
      • Protein disulfide isomerase associated 4 antibody
      • Protein disulfide isomerase family A member 4 antibody
      • Protein disulfide isomerase related protein antibody
      • Protein disulfide-isomerase A4 antibody
      • Protein ERp 72 antibody
      • Protein ERp72 antibody
      see all

    Anti-ERp72 antibody images

    • All lanes : Anti-ERp72 antibody (ab52841) at 1/1000 dilution

      Lane 1 : Total protein extract from E coli with ~50ng to 100ng of a tagged fusion protein of an irrelevant antigen
      Lane 2 : Total protein extract from E coli with ~50ng to 500ng of the antigen (tagged fusion protein)

      Lysates/proteins at 20 µg per lane.

      Secondary
      Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated, at 1/5000 dilution

      Predicted band size : 73 kDa

    References for Anti-ERp72 antibody (ab52841)

    ab52841 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab52841.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"