Anti-ETFDH antibody [3D1AC4AF3] (ab131376)

Overview

• Product nameAnti-ETFDH antibody [3D1AC4AF3]See all ETFDH primary antibodies ...
• Description
  Mouse monoclonal [3D1AC4AF3] to ETFDH
• Tested applicationsIn-Cell ELISA, Flow Cyt, ICC/IF, WB, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Rat, Cow, Human
  
• Immunogen

  Recombinant Human ETFDH protein.

• Positive controlWB: HLH, HHH, HeLa, HDFn, SY5Y, Jurkat, MLH, MHH, RLH, RHH, RBH, RKH, RSMH tissue homogenates
  ICC/IF: Paraformaldehyde fixed HDFn (Human), NIH 3T3 (Mouse) and H9C2 (Rat) cells
  Flow Cyt: HeLa cells This antibody gave a positive result in IHC in the following FFPE tissue: Human normal heart muscle.

Properties

• FormLiquid
• Storage instructionsStore at +4°C.
• Storage bufferPreservative: 0.02% Sodium azide
  Constituents: 0.357% HEPES, 0.877% Sodium chloride
• Concentration information loading...
• Clonality Monoclonal
• Clone number3D1AC4AF3
• IsotypeIgG1
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitochondrial markers

  • Signal Transduction
  • Metabolism
  • Mitochondrial

  • Tags & Cell Markers
  • Subcellular Markers
  • Organelles
  • Mitochondria

Applications

Target

• FunctionAccepts electrons from ETF and reduces ubiquinone.
• Involvement in diseaseDefects in ETFDH are the cause of glutaric aciduria type 2C (GA2C) [MIM:231680]. GA2C is an autosomal recessively inherited disorder of fatty acid, amino acid, and choline metabolism. It is characterized by multiple acyl-CoA dehydrogenase deficiencies resulting in large excretion not only of glutaric acid, but also of lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids.
• Sequence similaritiesBelongs to the ETF-QO/fixC family.
  Contains 1 4Fe-4S ferredoxin-type domain.
• Cellular localizationMitochondrion inner membrane.

Target information above from: UniProt accession Q16134 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 768074 Cow
  • Entrez Gene: 2110 Human
  • Entrez Gene: 66841 Mouse
  • Entrez Gene: 295143 Rat
  • Omim: 231675 Human
  • SwissProt: Q2KIG0 Cow
  • SwissProt: Q16134 Human
  • SwissProt: Q921G7 Mouse

  • SwissProt: Q6UPE1 Rat
  • Unigene: 155729 Human
  • Unigene: 28336 Mouse
  • Unigene: 37277 Rat

  see all

• Alternative names
  Electron transfer flavoprotein ubiquinone oxidoreductase antibodyElectron transfer flavoprotein-ubiquinone oxidoreductase antibodyelectron transferring flavoprotein dehydrogenase antibody

  Electron-transferring-flavoprotein dehydrogenase antibodyETF dehydrogenase antibodyETF QO antibodyETF ubiquinone oxidoreductase antibodyETF-QO antibodyETF-ubiquinone oxidoreductase antibodyETFD_HUMAN antibodyEtfdh antibodymitochondrial antibody

  see all

Anti-ETFDH antibody [3D1AC4AF3] images

• 

  Western blot - Anti-ETFDH antibody [3D1AC4AF3] (ab131376)
  All lanes : Anti-ETFDH antibody [3D1AC4AF3] (ab131376) at 1 µg/ml
  
  Lane 1 : Molecular weight marker
  Lane 2 : Human Liver Homogenate (HLH) at 10 µg
  Lane 3 : Human Heart Homogenate (HHH) at 10 µg
  Lane 4 : HepG2 Cell Lysate at 15 µg
  Lane 5 : Hela Cell Lysate at 15 µg
  Lane 6 : HDFn Cell Lysate at 15 µg
  Lane 7 : SY5Y Cell Lysate at 15 µg
  Lane 8 : Jurkat Cell Lysate at 15 µg
  Lane 9 : Mouse Liver Homogenate (MLH) at 10 µg
  Lane 10 : Mouse Heart Homogenate (MHH) at 10 µg
  Lane 11 : Rat Liver Homogenate (RLH) at 10 µg
  Lane 12 : Rat Heart Homogenate (RHH) at 10 µg
  Lane 13 : Rat Brain Homogenate (RBH) at 10 µg
  Lane 14 : Rat Kidney Homogenate (RLH) at 10 µg
  Lane 15 : Rat Skeletal Muscle Homogenate (RSMH) at 10 µg
  
  Secondary
  Goat anti-Mouse AP at 1/3000 dilution
  
  Predicted band size : 68 kDa
  
• 

  Immunocytochemistry/ Immunofluorescence - Anti-ETFDH antibody [3D1AC4AF3] (ab131376)
  Immunofluorescent analysis of ETFDH in HDFn cells.
  The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were then incubated with ab131376 at 5 µg/ml for 2 h at room temperature, or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1 h. 1% BSA was used as the blocking agent for all blocking steps. The target protein locates to the mitochondria.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFDH antibody [3D1AC4AF3] (ab131376)

  IHC image of ETFDH staining in Human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab131376, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• 

  Flow Cytometry - Anti-ETFDH antibody [3D1AC4AF3] (ab131376)
  Flow cytometric analysis of ETFDH in HeLa cells fixed and permeabilized with methanol and stained with 1 µg/mL of ab131376 (blue), or an equal amount of an isotype control antibody (red). 1% BSA was used as the blocking reagent for all the blocking steps.