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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab28457? Please let us know so that we can cite the reference in this datasheet
ab28457 has been referenced in 1 publications.
- Koos B et al. The transcription factor evi-1 is overexpressed, promotes proliferation, and is prognostically unfavorable in infratentorial ependymomas. Clin Cancer Res 17:3631-7 (2011). WB, ICC/IF, IHC-P; Human. PubMed: 21493867
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - EVI1 antibody (ab28457)
Anti-EVI1 antibody (ab28457) at 0.5 µg/ml + fetal lung lysate at 10 µg
Secondary
HRP conjugated anti rabbit IgG at 1/50000 dilution
Predicted band size : 118 kDa
Observed band size : 118 kDa
Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Paraffin-embedded sections) - EVI1 antibody (ab28457)
Ab28457, at a dilution of 4.0-8.0 µg/ml, staining EV11 in paraffin embedded human intrestinal tissue by Immunohistochemistry.
Cells with positive label: epithelial cells of intestinal villus (indicated with arrows).
Magnification: 400X.
Immunocytochemistry/ Immunofluorescence-EVI1 antibody(ab28457)
ICC/IF image of ab28457 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28457, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
EVI1 antibody for Western blot in Mouse (28457)
EVI1 antibody for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Human (28457)
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