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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - EVI1 antibody (ab28457)
Anti-EVI1 antibody (ab28457) at 0.5 µg/ml + fetal lung lysate at 10 µg
Secondary
HRP conjugated anti rabbit IgG at 1/50000 dilution
Predicted band size : 118 kDa
Observed band size : 118 kDa
Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Paraffin-embedded sections) - EVI1 antibody (ab28457)
Ab28457, at a dilution of 4.0-8.0 µg/ml, staining EV11 in paraffin embedded human intrestinal tissue by Immunohistochemistry.
Cells with positive label: epithelial cells of intestinal villus (indicated with arrows).
Magnification: 400X.
Immunocytochemistry/ Immunofluorescence-EVI1 antibody(ab28457)
ICC/IF image of ab28457 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28457, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
EVI1 antibody for Western blot in Mouse (28457)
EVI1 antibody for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Human (28457)
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