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Western blot - EVI1 antibody (ab28457)
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Immunohistochemistry (Paraffin-embedded sections) - EVI1 antibody (ab28457)
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Product Name
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EVI1 antibody
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See all EVI1 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to EVI1
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Immunogen
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Synthetic peptide derived from residues 964 - 1013 of human EVI1.
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Reacts with
(species key)
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Hu
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Specificity
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Ab28457 recognises Ecotropic virus integration 1 site protein(EVI1)
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/12500.
IHC-P: Use at a concentration of 4 - 8 µg/ml.
WB: Use at a concentration of 0.5 µg/ml. Predicted molecular weight: 118 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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WB: fetal lung lysate.
IHC-P: human intestine tissue.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Chromatin and Nuclear Signaling >> Cell cycle >> Cell Differentiation
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Relevance
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EVI1 promotes cell proliferation by interacting with BRG1 and blocking the repression of BRG1 on E2F1 activity. EVI1 may be involved in megakaryocytic differentiation.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS.
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Purification notes
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Ab28457 was purified by peptide affinity chromatography method.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this EVI1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for EVI1 antibody images, references, products related to ab28457 and other tools.
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EVI1 antibody images:
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 Western blot - EVI1 antibody (ab28457)
EVI1 antibody (ab28457) at 0.5 µg/ml + fetal lung lysate at 10 µg
Secondary
HRP conjugated anti rabbit IgG at 1/50000 dilution
Predicted band size : 118 kDa
Observed band size : 118 kDa
Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.
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Immunohistochemistry (Paraffin-embedded sections) - EVI1 antibody (ab28457)
Ab28457, at a dilution of 4.0-8.0 µg/ml, staining EV11 in paraffin embedded human intrestinal tissue by Immunohistochemistry.
Cells with positive label: epithelial cells of intestinal villus (indicated with arrows).
Magnification: 400X.
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Search PubMed (MEDLINE) for references to EVI1
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EVI1 antibody - more information
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Customer reviews (feedback) regarding EVI1 antibody |
Customer FAQs regarding EVI1 antibody |
Protocols for EVI1 antibody |
Price and availability of products related to EVI1 antibody |
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EVI1 antibody - related products:
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EVI1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about EVI1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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