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Anti-EVI1 antibody (ab86306) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal brain lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 118 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
Gel concentration 6-18%
1
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