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Products:Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Overview
Product name
Anti-EXOC7 antibody
See all EXOC7 products (6) ...
Description
Mouse monoclonal to EXOC7
Tested applications
WB, IHC-P, IP, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment: VFQPGVKLRD KERQIIKERF KGFNDGLEEL CKIQKAWAIP DTEQRDRIRQ AQKTIVKETY GAFLQKFGSV PFTKNPEKYI KYGVEQVGDM IDRLFDTSA, corresponding to amino acids 586-685 of Human EXOC7
VFQPGVKLRD KERQIIKERF KGFNDGLEEL CKIQKAWAIP DTEQRDRIRQ AQKTIVKETY GAFLQKFGSV PFTKNPEKYI KYGVEQVGDM IDRLFDTSA
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG2a
Light chain type
kappa
Research areas
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab57402 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml.Predicted molecular weight: 83 kDa.
IHC-P: Use a concentration of 3 µg/ml
IP: Use at an assay dependent concentration. (PubMed: 21566143)
ICC/IF: Use at an assay dependent dilution. (PubMed: 21108927)
Target
Function
Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. In adipocytes, plays a crucial role in targeting SLC2A4 vesicle to the plasma membrane in response to insulin, perhaps directing the vesicle to the precise site of fusion.
Sequence similarities
Belongs to the EXO70 family.
Domain
The N-terminus is involved in SEC8 and ARHQ binding.
The C-terminus is required for translocation to the plasma membrane.
Cellular localization
Cytoplasm > cytosol. Cell membrane. Translocates, as a preformed complex with SEC6 and SEC8, to the plasma membrane in response to insulin through the activation of ARHQ.
Target information above from: UniProt accessionQ9UPT5
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 2 5 3p antibody
- DKFZp686J04253 antibody
- EX070 antibody
see all
Database links
- Entrez Gene: 23265 Human
- Omim: 608163 Human
- SwissProt: Q9UPT5 Human
- Unigene: 514496 Human
- Unigene: 533985 Human
Anti-EXOC7 antibody images:
IHC-P - EXOC7 antibody (ab57402)
EXOC7 antibody (ab57402) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human small Intestine.
Western blot - EXOC7 antibody (ab57402)
EXOC7 antibody (ab57402) at 1ug/lane + HeLa cell lysate at 25ug/lane.
References for Anti-EXOC7 antibody (ab57402)
This product has been referenced in:
- Hubert Tet al. Actin and Arp2/3 localize at the centrosome of interphase cells. Biochem Biophys Res Commun 404:153-8 (2011). ICC/IF; Human.Read more (PubMed: 21108927) »
- Barkefors Iet al. Exocyst Complex Component 3-like 2 (EXOC3L2) Associates with the Exocyst Complex and Mediates Directional Migration of Endothelial Cells. J Biol Chem 286:24189-99 (2011). IHC-P, IP; Mouse.Read more (PubMed: 21566143) »
See all 3 publications for this product
Publishing research using ab57402? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"