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Anti-EXOSC5 antibody (ab69699) at 1/500 dilution + human kidney lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 25 kDa
Observed band size : 27 kDa (why is the actual band size different from the predicted?)
All lanes : Anti-EXOSC5 antibody (ab69699) at 1/500 dilution
Lane 1 : EXOSC5 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 25 kDa
Observed band size : 27 kDa (why is the actual band size different from the predicted?)
ab69699 at 10µg/ml staining EXOSC5 in human Hela cells by ICC/IF.
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