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Western blot - EXOSC5 antibody (ab69699)
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Western blot - EXOSC5 antibody (ab69699)
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Immunocytochemistry/ Immunofluorescence - EXOSC5 antibody (ab69699)
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Product Name
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EXOSC5 antibody
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Product type
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Primary antibodies
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Description
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Mouse polyclonal to EXOSC5
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Immunogen
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Full length protein (Human), EXOSC5 (AAH07742.1).
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 10 µg/ml.
WB: 1/500 - 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 25 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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human kidney tissue lysate, EXOSC5 transfected 293T cell lysate.
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Cellular localization
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Nuclear, nucleolus.
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
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Relevance
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EXOSC5 is a component of the exosome 3'->5' exoribonuclease complex, a complex that degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3'-untranslated regions. It is required for the 3'-processing of the 7S pre-RNA to the mature 5.8S rRNA and has a 3'-5' exonuclease activity.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
Constituents: 1X PBS, pH 7.2
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this EXOSC5 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for EXOSC5 antibody images, references, products related to ab69699 and other tools.
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EXOSC5 antibody images:
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 Western blot - EXOSC5 antibody (ab69699)
EXOSC5 antibody (ab69699) at 1/500 dilution + human kidney lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 25 kDa
Observed band size : 27 kDa (why is the actual band size different from the predicted?)
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Western blot - EXOSC5 antibody (ab69699)
All lanes : EXOSC5 antibody (ab69699) at 1/500 dilution
Lane 1 : EXOSC5 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 25 kDa
Observed band size : 27 kDa (why is the actual band size different from the predicted?)
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Immunocytochemistry/ Immunofluorescence - EXOSC5 antibody (ab69699)
ab69699 at 10µg/ml staining EXOSC5 in human Hela cells by ICC/IF.
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Search PubMed (MEDLINE) for references to EXOSC5
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EXOSC5 antibody - more information
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Customer reviews (feedback) regarding EXOSC5 antibody |
Customer FAQs regarding EXOSC5 antibody |
Protocols for EXOSC5 antibody |
Price and availability of products related to EXOSC5 antibody |
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EXOSC5 antibody - related products:
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EXOSC5 antibody - other tools:
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Contact Abcam with a Technical Enquiry about EXOSC5 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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