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The reagents in this kit constitute a biotin-free immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a secondary antibody-HRP conjugate which reacts with the primary antibody, and substrate-chromogen (DAB).
This kit is suitable for staining animal tissues, except mouse and rabbit tissues. The polyvalent-HRP in the kit contains anti-mouse and anti-rabbit secondary antibodies (conjugated with HRP), so the secondary Ab-HRP will non-specifically bind to mouse and rabbit tissues.
DAB is a suspected carcinogen. Handle with care.
Contains hydrogen peroxide.
|Components||15 ml||60 ml||125 ml||60 ml|
|50x DAB Chromogen||1 x 0.5ml||1 x 2ml||1 x 4ml||1 x 2ml|
|DAB substrate||1 x 15ml||1 x 60ml||1 x 125ml||1 x 60ml|
|Goat anti-rabbit HRP Conjugate||1 x 15ml||1 x 60ml||1 x 125ml||1 x 60ml|
|Hydrogen Peroxide Block||1 x 15ml||1 x 60ml||1 x 125ml||1 x 60ml|
|Mouse Specifying Reagent (Complement)||1 x 15ml||1 x 60ml||1 x 125ml||1 x 60ml|
|Protein block||1 x 15ml||1 x 60ml||1 x 125ml||1 x 60ml|
Our Abpromise guarantee covers the use of ab80436 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent dilution.|
ab1220 staining human kidney sections by IHC-P using EXPOSE IHC detection kit ab80436. Formalin fixed paraffin embedded tissue sections were pre-treated using heat mediated antigen retrieval (using a pressure cooker) with sodium citrate buffer (pH 6) for 30 minutes. The section was incubated with ab1220, 5 µg/ml, for 1 hour at room temperature. DAB was used as the chromogen and the section was counterstained with haematoxylin and mounted with DPX.
Formalin-fixed paraffin-embedded human glioma tissue sections were stained with anti-methyl-CpG DNA binding protein (MBD2) antibody as per EXPOSE kit protocol. Image is customer-generated data. Sample is 5 micron.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of chicken E7 brain tissue sections staining SOX2. Primary antibody ab97959 at dilution 1/5000 using EXPOSE IHC detection kit ab80436.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"