EXPOSE Rabbit specific HRP/DAB detection IHC Kit (ab80437)
- Datasheet
- Specific References (8)
- Protocols
Overview
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Product nameEXPOSE Rabbit specific HRP/DAB detection IHC Kit
See all HRP/DAB detection kit kits -
Detection methodColorimetric
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Assay typeMicro-Polymer
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Product overview
The reagents in this kit constitute a biotin-free immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a rabbit specific HRP conjugate which reacts with the primary antibody, and substrate-chromogen (DAB).
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Notes
DAB is a suspected carcinogen. Handle with care.
Contains hydrogen peroxide.
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Tested applicationsSuitable for: IHC-Pmore details
Properties
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Storage instructionsStore at +4°C. Please refer to protocols.
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Components 15 ml 60 ml 125 ml 50x DAB Chromogen 1 x 1ml 1 x 2ml 1 x 4ml DAB substrate 1 x 15ml 1 x 60ml 1 x 125ml Goat anti-rabbit HRP Conjugate 1 x 15ml 1 x 60ml 1 x 125ml Hydrogen Peroxide Block 1 x 15ml 1 x 60ml 1 x 125ml Protein block 1 x 15ml 1 x 60ml 1 x 125ml -
Research areas
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RelevanceHRP/DAB detection kit is a immunoenzymatic antigen detection system for immunohistochemistry techniques.
Applications
Our Abpromise guarantee covers the use of ab80437 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent dilution. |
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)Hall OJ et al. PLoS Pathog. 12:e1005840 (2016)Immunohistochemical analysis of mouse lung tissue labeling Ki67 detected with ab80437. Tissue sections were embedded in paraffin and were counterstained with hematoxylin.
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Immunohistochemistry FrozenSelby MJ et al. PLoS One (2016)Immunohistochemical (frozen) analysis of MC38 tumor sections labeling CD3 with ab16669 at 1/400 dilution. Sections were fixed with acetone, treated with peroxidase block to quench endogenous peroxidase, and then further blocked with a 10% goat serum and 5% BSA solution. CD3 positive T cells were detected using ab80437. Invasive margin (top) shows sections derived from the periphery of the tumor and interior (bottom) shows sections from within the tumor.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EXPOSE Rabbit Specific HRP/DAB Detection IHC kit (ab80437)ab18723 staining 6 week rat brain tissue subventricular zone (SVZ) by IHC-P using rabbit-specific EXPOSE IHC detection kit (ab80437). Formalin fixed paraffin embedded tissue sections were pre-treated using heat mediated antigen retrieval (using a pressure cooker) with sodium citrate buffer (pH6) for 30 mins. The section was incubated with ab18723, 0.1µg/ml, for 1 hour at room temperature. DAB was used as the chromogen and the section was counterstained with haematoxylin and mounted with DPX.
References
This product has been referenced in:
- Snow WM et al. In Vivo Detection of Gray Matter Neuropathology in the 3xTg Mouse Model of Alzheimer's Disease with Diffusion Tensor Imaging. J Alzheimers Dis 58:841-853 (2017). Read more (PubMed: 28505976) »
- Woolf N et al. Targeting tumor multicellular aggregation through IGPR-1 inhibits colon cancer growth and improves chemotherapy. Oncogenesis 6:e378 (2017). IHC-P ; Human . Read more (PubMed: 28920928) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"