• Product name
    EXPOSE Rabbit specific HRP/DAB detection IHC Kit
    See all HRP/DAB detection kit kits
  • Detection method
  • Assay type
  • Product overview

    The reagents in this kit constitute a biotin-free immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a rabbit specific HRP conjugate which reacts with the primary antibody, and substrate-chromogen (DAB).

  • Notes

    DAB is a suspected carcinogen. Handle with care.

    Contains hydrogen peroxide.

  • Tested applications
    Suitable for: IHC-Pmore details


  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 15 ml 60 ml 125 ml
    50x DAB Chromogen 1 x 1ml 1 x 2ml 1 x 4ml
    DAB substrate 1 x 15ml 1 x 60ml 1 x 125ml
    Goat anti-rabbit HRP Conjugate 1 x 15ml 1 x 60ml 1 x 125ml
    Hydrogen Peroxide Block 1 x 15ml 1 x 60ml 1 x 125ml
    Protein block 1 x 15ml 1 x 60ml 1 x 125ml
  • Research areas
  • Relevance
    HRP/DAB detection kit is a immunoenzymatic antigen detection system for immunohistochemistry techniques.


Our Abpromise guarantee covers the use of ab80437 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.


  • Immunohistochemical analysis of mouse lung tissue labeling Ki67 detected with ab80437. Tissue sections were embedded in paraffin and were counterstained with hematoxylin. 


  • Immunohistochemical (frozen) analysis of MC38 tumor sections labeling CD3 with ab16669 at 1/400 dilution. Sections were fixed with acetone, treated with peroxidase block to quench endogenous peroxidase, and then further blocked with a 10% goat serum and 5% BSA solution. CD3 positive T cells were detected using ab80437. Invasive margin (top) shows sections derived from the periphery of the tumor and interior (bottom) shows sections from within the tumor.






  • ab18723 staining 6 week rat brain tissue subventricular zone (SVZ) by IHC-P using rabbit-specific EXPOSE IHC detection kit (ab80437). Formalin fixed paraffin embedded tissue sections were pre-treated using heat mediated antigen retrieval (using a pressure cooker) with sodium citrate buffer (pH6) for 30 mins. The section was incubated with ab18723, 0.1µg/ml, for 1 hour at room temperature. DAB was used as the chromogen and the section was counterstained with haematoxylin and mounted with DPX.


This product has been referenced in:
  • Snow WM  et al. In Vivo Detection of Gray Matter Neuropathology in the 3xTg Mouse Model of Alzheimer's Disease with Diffusion Tensor Imaging. J Alzheimers Dis 58:841-853 (2017). Read more (PubMed: 28505976) »
  • Woolf N  et al. Targeting tumor multicellular aggregation through IGPR-1 inhibits colon cancer growth and improves chemotherapy. Oncogenesis 6:e378 (2017). IHC-P ; Human . Read more (PubMed: 28920928) »

See all 7 Publications for this product

Customer reviews and Q&As

Thank you for calling Abcam earlier today. I have had a response from the lab about the storage life of the kit, ab80437, the EXPOSE Rabbit specific HRP/DAB detection IHC kit. They have told me that the storage life of this kit is 12-18 months if ...

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