Overview

  • Product nameAnti-EZH1 antibodySee all EZH1 primary antibodies ...
  • Description
    Rabbit polyclonal to EZH1
  • SpecificityThis antibody detects endogenous levels of total EZH1 protein.
  • Tested applicationsWB, IHC-P, ELISA more details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (Human) from an internal region of EZH1.

  • Positive control
    • WB: Extracts from HT-29 cells, LOVO cells, A549 cells, NIH-3T3 cells, RAW264.7 cells and COS-7 cells. IHC-P: human brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab64850 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/500 - 1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
IHC-P 1/50 - 1/100.
ELISA 1/5000.

Target

  • FunctionPolycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH1-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.
  • Sequence similaritiesBelongs to the histone-lysine methyltransferase family. EZ subfamily.
    Contains 1 SET domain.
  • Cellular localizationNucleus. Colocalizes with trimethylated 'Lys-27' of histone H3.
  • Target information above from: UniProt accession Q92800 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Enhancer of zeste homolog 1 (Drosophila) antibody
    • Enhancer of zeste homolog 1 antibody
    • ENX 2 antibody
    • ENX-2 antibody
    • ENX2 antibody
    • EZH 1 antibody
    • Ezh1 antibody
    • EZH1_HUMAN antibody
    • Histone-lysine N-methyltransferase EZH1 antibody
    • KIAA0388 antibody
    see all

Anti-EZH1 antibody images

  • All lanes : Anti-EZH1 antibody (ab64850) at 1/500 dilution

    Lane 1 : Extracts from HT-29 cells
    Lane 2 : Extract from LOVO cells
    Lane 3 : Extracts from A549 cells
    Lane 4 : Extracts from NIH-3T3 cells
    Lane 5 : Extracts from RAW264.7 cells
    Lane 6 : Extracts from COS-7 cells
    Lane 7 : Extracts from COS-7 cells with blocking peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 85 kDa
    Observed band size : 85 kDa
  • Ab64850 (1/50 dilution) staining EZH1 in paraffin embedded human brain tissue by immunohistochemistry.

    The left panel is in the absence of blocking peptide, whereas the right panel is in the presence of blocking peptide.

References for Anti-EZH1 antibody (ab64850)

This product has been referenced in:
  • Wu H  et al. Dual functions of Tet1 in transcriptional regulation in mouse embryonic stem cells. Nature 473:389-93 (2011). Read more (PubMed: 21451524) »
  • Sdek P  et al. Rb and p130 control cell cycle gene silencing to maintain the postmitotic phenotype in cardiac myocytes. J Cell Biol 194:407-23 (2011). WB ; Mouse . Read more (PubMed: 21825075) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us. We have three antibodies that may be suitable for your purposes:

ab13665: rabbit polyclonal to EZH1 validated in WB on mouse and human samples
ab86128: rabbit polyclonal to EZH1 validated in WB and ICC/IF on ...

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Thank you for sending the image of your blots.

When you use the PVDF membrane, are you pre-wetting it with methanol? Because PVDF is hydrophobic, it may not properly bind the blocking agent without the pre-wetting step. This could potentially...

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Thank you for your phone call. As we discussed, it is possible that your 6 x 15 minute washes are washing the blocking agent off your membrane. It may help to reduce the wash time to 3 x 5 min, shorten the primary antibody incubation to 1 hour at RT, a...

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Thank you for contacting us. We do not have tests against purified Ezh1 or Ezh2 but a BLAST search of the immunogen sequence returns only Ezh1, so it is expected that the antibody is not capable of detecting Ezh2. I hope this information is helpf...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"