Anti-Eg5 antibody (ab61199)
Key features and details
- Rabbit polyclonal to Eg5
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Eg5 antibody
See all Eg5 primary antibodies -
Description
Rabbit polyclonal to Eg5 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic non-phosphopeptide derived from human Eg5 around the phosphorylation site of threonine 927.
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Positive control
- Human lung carcinoma tissue, COLO205 cell extracts and HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab61199 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab61199 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/100.
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WB |
1/500 - 1/1000. Detects a band of approximately 120 kDa (predicted molecular weight: 120 kDa).
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ICC/IF |
1/500 - 1/1000.
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Notes |
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IHC-P
1/50 - 1/100. |
WB
1/500 - 1/1000. Detects a band of approximately 120 kDa (predicted molecular weight: 120 kDa). |
ICC/IF
1/500 - 1/1000. |
Target
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Function
Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays. -
Involvement in disease
Defects in KIF11 are the cause of microcephaly with or without chorioretinopathy, lymphedema, or mental retardation (MCLMR) [MIM:152950]. An autosomal dominant disorder that involves an overlapping but variable spectrum of central nervous system and ocular developmental anomalies. Microcephaly ranges from mild to severe and is often associated with mild to moderate developmental delay and a characteristic facial phenotype with upslanting palpebral fissures, broad nose with rounded tip, long philtrum with thin upper lip, prominent chin, and prominent ears. Chorioretinopathy is the most common eye abnormality, but retinal folds, microphthalmia, and myopic and hypermetropic astigmatism have also been reported, and some individuals have no overt ocular phenotype. Congenital lymphedema, when present, is typically confined to the dorsa of the feet, and lymphoscintigraphy reveals the absence of radioactive isotope uptake from the webspaces between the toes. -
Sequence similarities
Belongs to the kinesin-like protein family. BimC subfamily.
Contains 1 kinesin-motor domain. -
Post-translational
modificationsPhosphorylated exclusively on serine during S phase, but on both serine and Thr-926 during mitosis, so controlling the association of KIF11 with the spindle apparatus (probably during early prophase). Phosphorylated upon DNA damage, probably by ATM or ATR.
A subset of this protein primarily localized at the spindle pole is phosphorylated by NEK6 during mitosis; phosphorylation is required for mitotic function. -
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton > spindle pole. - Information by UniProt
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Database links
- Entrez Gene: 3832 Human
- Entrez Gene: 16551 Mouse
- Omim: 148760 Human
- SwissProt: P52732 Human
- SwissProt: Q6P9P6 Mouse
- Unigene: 8878 Human
- Unigene: 42203 Mouse
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Alternative names
- EG5 antibody
- HKSP antibody
- KIF11 antibody
see all
Images
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All lanes : Anti-Eg5 antibody (ab61199) at 1/500 dilution
Lane 1 : COLO205 cell extracts
Lane 2 : COLO205 cell extracts with immunising peptide
Predicted band size: 120 kDa
Observed band size: 120 kDa -
ab61199 at 1/50 dilution staining Eg5 in human lung carcinoma by Immunohistochemistry, Paraffin-embedded tissue, in the absence (left) or presence (right) of the immunising peptide.
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ab61199 at 1/500 dilution staining Eg5 in HeLa cells, by Immunofluorescence, in the absence (left) or presence (right) of the immunising peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab61199 has been referenced in 11 publications.
- Hu ZD et al. KIF11 Promotes Proliferation of Hepatocellular Carcinoma among Patients with Liver Cancers. Biomed Res Int 2021:2676745 (2021). PubMed: 33490265
- Mo XC et al. Screening and identification of hub genes in bladder cancer by bioinformatics analysis and KIF11 is a potential prognostic biomarker. Oncol Lett 21:205 (2021). PubMed: 33574944
- Serra-Marques A et al. Concerted action of kinesins KIF5B and KIF13B promotes efficient secretory vesicle transport to microtubule plus ends. Elife 9:N/A (2020). PubMed: 33174839
- Jin Q et al. High kinesin family member 11 expression predicts poor prognosis in patients with clear cell renal cell carcinoma. J Clin Pathol 72:354-362 (2019). PubMed: 30819726
- Xu X et al. Redox-Responsive Nanoparticle-Mediated Systemic RNAi for Effective Cancer Therapy. Small 14:e1802565 (2018). PubMed: 30230235