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WB with mouse brain tissue |
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Egr1 antibody (ab54966) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human placenta.
Overlay histogram showing HeLa cells stained with ab54966 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54966, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (
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