Overview

  • Product nameAnti-Egr1 antibodySee all Egr1 primary antibodies ...
  • Description
    Mouse monoclonal to Egr1
  • Tested applicationsWB, IHC-P, Flow Cyt more details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment: SPVATSYPSP VTTSYPSPAT TSYPSPVPTS FSSPGSSTYP SPVHSGFPSP SVATTYSSVP PAFPAQVSSF PSSAVTNSFS ASTGLSDMTA TFSPRTIEIC , corresponding to amino acids 444-544 of Human Egr1

Properties

Applications

Our Abpromise guarantee covers the use of ab54966 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 58 kDa.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 1µg for 106 cells.

Target

  • FunctionTranscriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation.
  • Sequence similaritiesBelongs to the EGR C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • Cellular localizationNucleus.
  • Target information above from: UniProt accession P18146 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • AT 225 antibody
    • AT225 antibody
    • Early growth response 1 antibody
    • Early growth response protein 1 antibody
    • EGR 1 antibody
    • EGR-1 antibody
    • EGR1 antibody
    • EGR1_HUMAN antibody
    • G0S 30 antibody
    • G0S30 antibody
    • KROX 24 antibody
    • Krox 24 protein antibody
    • KROX24 antibody
    • Nerve growth factor induced protein A antibody
    • Nerve growth factor-induced clone A antibody
    • Nerve growth factor-induced protein A antibody
    • NGFI A antibody
    • NGFI-A antibody
    • NGFIA antibody
    • TIS 8 antibody
    • TIS8 antibody
    • Transcription factor ETR 103 antibody
    • Transcription factor ETR103 antibody
    • Transcription factor Zif 268 antibody
    • Transcription factor Zif268 antibody
    • Zfp 6 antibody
    • ZIF 268 antibody
    • ZIF268 antibody
    • Zinc finger protein 225 antibody
    • Zinc finger protein Krox-24 antibody
    • ZNF 225 antibody
    • ZNF225 antibody
    see all

Anti-Egr1 antibody images

  • All lanes : Anti-Egr1 antibody (ab54966)

    Lane 1 : EGR1 transfected lysate
    Lane 2 : non-transfected


    Predicted band size : 58 kDa
  • Egr1 antibody (ab54966) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human placenta.
  • Overlay histogram showing HeLa cells stained with ab54966 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54966, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-Egr1 antibody (ab54966)

This product has been referenced in:
  • Bakalash S  et al. Egr1 expression is induced following glatiramer acetate immunotherapy in rodent models of glaucoma and Alzheimer's disease. Invest Ophthalmol Vis Sci 52:9033-46 (2011). Read more (PubMed: 21969301) »
  • Bieging KT  et al. A shared gene expression signature in mouse models of EBV-associated and non-EBV-associated Burkitt lymphoma. Blood 118:6849-59 (2011). WB . Read more (PubMed: 22039254) »

See all 3 Publications for this product

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