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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Overview
Product name
Anti-Egr1 antibody
See all Egr1 products (4) ...
Description
Mouse monoclonal to Egr1
Tested applications
WB, IHC-P, Flow Cytmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Recombinant fragment: SPVATSYPSP VTTSYPSPAT TSYPSPVPTS FSSPGSSTYP SPVHSGFPSP SVATTYSSVP PAFPAQVSSF PSSAVTNSFS ASTGLSDMTA TFSPRTIEIC , corresponding to amino acids 444-544 of Human Egr1
SPVATSYPSP VTTSYPSPAT TSYPSPVPTS FSSPGSSTYP SPVHSGFPSP SVATTYSSVP PAFPAQVSSF PSSAVTNSFS ASTGLSDMTA TFSPRTIEIC
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG2a
Light chain type
kappa
Research areas
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Applications
Show applications key
Our Abpromise guarantee covers the use of ab54966 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml.Predicted molecular weight: 58 kDa.
IHC-P: Use a concentration of 3 µg/ml
Flow Cyt: Use 1µg for 106 cells.
Target
Function
Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation.
Sequence similarities
Belongs to the EGR C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP18146
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AT 225 antibody
- AT225 antibody
- Early growth response 1 antibody
see all
Database links
- Entrez Gene: 1958 Human
- Entrez Gene: 13653 Mouse
- Omim: 128990 Human
- SwissProt: P18146 Human
- SwissProt: P08046 Mouse
- Unigene: 326035 Human
- Unigene: 708393 Human
- Unigene: 181959 Mouse
Anti-Egr1 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Egr1 antibody (ab54966)
Egr1 antibody (ab54966) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human placenta.
Flow Cytometry-Egr1 antibody(ab54966)
Overlay histogram showing HeLa cells stained with ab54966 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54966, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
References for Anti-Egr1 antibody (ab54966)
This product has been referenced in:
- Bakalash Set al. Egr1 expression is induced following glatiramer acetate immunotherapy in rodent models of glaucoma and Alzheimer's disease. Invest Ophthalmol Vis Sci 52:9033-46 (2011).Read more (PubMed: 21969301) »
- Bieging KTet al. A shared gene expression signature in mouse models of EBV-associated and non-EBV-associated Burkitt lymphoma. Blood 118:6849-59 (2011). WB.Read more (PubMed: 22039254) »
See all 3 publications for this product
Publishing research using ab54966? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"