Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
This fast track antibody is not yet fully characterised. It is subject to these terms and conditions
Overview
Product name
Anti-Egr1 antibody
See all Egr1 products (4) ...
Description
Rabbit polyclonal to Egr1
This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »
Cross reactivity
Predicted to work with
Mouse, Rat, Human
Immunogen
Synthetic peptide: GEQPYEHLTAESFPDI-C conjugated to KLH, corresponding to amino acids 93-108 of Human Egr1.
GEQPYEHLTA ESFPDI-C
General notes
Expiration date is 1 year from date of opening.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Applications
Application notes
Tested in peptide ELISA.
We have detected a band of 58kDa in mouse embryonic fibroblast whole cell lysate, but we have failed to detect a band at 83kDa (expected size from data from other antibodies). We would appreciate any customer feedback for this antibody.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation.
Sequence similarities
Belongs to the EGR C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP18146
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AT225 antibody
- Early growth response 1 antibody
- Early growth response protein 1 antibody
see all
Database links
- Entrez Gene: 1958 Human
- Entrez Gene: 13653 Mouse
- Entrez Gene: 24330 Rat
- Omim: 128990 Human
- SwissProt: P18146 Human
- SwissProt: P08046 Mouse
- SwissProt: P08154 Rat
- Unigene: 326035 Human
see all
Anti-Egr1 antibody images:
This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.
Western blot - Egr1 antibody (ab6054)
Anti-Egr1 antibody (ab6054) at 1/1500 dilution + Mouse embryonic fibroblast whole cell lysate at 35 µg
Secondary
Goat-anti-Rabbit IgG [H&L] at 1/10000 dilution
Observed band size : 58 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Egr1 antibody (ab6054)
ab6054 at 10 µg/ml staining human ovarian cancinoma.
References for Anti-Egr1 antibody (ab6054)
This product has been referenced in:
- Nadra Ket al. Phosphatidic acid mediates demyelination in Lpin1 mutant mice. Genes Dev 22:1647-61 (2008). WB; Mouse.Read more (PubMed: 18559480) »
See 1 publication for this product
Publishing research using ab6054? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"