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Western blot - Egr1 antibody (ab6054)
Western blot - Egr1 antibody (ab6054)
(enlarge)


Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Egr1 antibody (ab6054)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Egr1 antibody (ab6054)
(enlarge)


This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

Product Name 

Egr1 antibody

 

See all Egr1 antibodies (3)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Egr1

 

  Note on antibody grade

Fast-Track antibodies

  • Immunogen affinity purified
  • high ELISA titre values
  • released early (to accelerate your research)
  • 950 Abpoints to the 1st researcher
    who submits conclusive data (positive or negative):
    Read the Fast-Track antibody Terms of Use


Immunogen 

Synthetic peptide: GEQPYEHLTAESFPDI-C conjugated to KLH, corresponding to amino acids 93-108 of Human Egr1.
BLAST 'GEQPYEHLTAESFPDI-C' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Predicted to react 
(species key)

Hu
(Due to sequence homology)


Abreviews 

Customer reviews
(see key)


Read about Abreviews

Submit an Abreview

Be the first to review this antibody and earn 950 Abpoints.


Read Fast Track antibody terms and conditions.


Application notes 
(see key)

Recommended dilutions
Tested in peptide ELISA.
We have detected a band of 58kDa in mouse embryonic fibroblast whole cell lysate, but we have failed to detect a band at 83kDa (expected size from data from other antibodies). We would appreciate any customer feedback for this antibody.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear

Research areas 

Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger

Relevance 

This protein belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppressor gene.

 

Alternative names Egr1 antibody (ab6054)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

1958    (Human)
13653    (Mouse)
24330    (Rat)

GeneCard

    

GC05P137832    (Human)

Omim

    

128990    (Human)

SwissProt

    

P18146    (Human)
P08046    (Mouse)
P08154    (Rat)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Batch dependent within range: 0.93 - 0.93 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

Find concentration of your lot :

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

Expiration date is 1 year from date of opening.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Egr1 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for Egr1 antibody images, references, products related to ab6054 and other tools.

 

Egr1 antibody images:

This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.
Please read the definition of 'Fast-Track antibody'

 

Product image  Western blot - Egr1 antibody (ab6054)

Western blot - Egr1 antibody (ab6054)

Egr1 antibody (ab6054) at 1/1500 dilution + Mouse embryonic fibroblast whole cell lysate at 35 µg

Secondary
Goat-anti-Rabbit IgG [H&L] at 1/10000 dilution

Observed band size : 58 kDa (why is the actual band size different from the predicted?)

 

Product image  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Egr1 antibody (ab6054)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Egr1 antibody (ab6054)

ab6054 at 10 µg/ml staining human ovarian cancinoma.

 

References for Egr1 antibody (ab6054)

Specific publication references   This product has been used in:

Nadra K et al. Phosphatidic acid mediates demyelination in Lpin1 mutant mice. Genes Dev 22:1647-61 (2008). WB; Mouse. PubMed: 18559480

If you publish research using ab6054 please let us know so that we can cite the reference on this datasheet.

 

Search PubMed (MEDLINE) for references to Egr1

 

Egr1 antibody - more information

Customer reviews (feedback) regarding Egr1 antibody

Customer FAQs regarding Egr1 antibody

Protocols for Egr1 antibody

Price and availability of products related to Egr1 antibody

 

Egr1 antibody - related products:

 

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Other Egr1 Antibodies

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Egr1 antibody - other tools:

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