Anti-Egr1 antibody (ab6054)
- Product nameAnti-Egr1 antibodySee all Egr1 primary antibodies ...
- Species reactivity
Predicted to work with: Mouse, Rat, Human
Synthetic peptide: GEQPYEHLTAESFPDI-C conjugated to KLH, corresponding to amino acids 93-108 of Human Egr1.
- General notesExpiration date is 1 year from date of opening.
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
- Concentration information loading...
- PurityImmunogen affinity purified
- Clonality Polyclonal
- Research Areas
We have detected a band of 58kDa in mouse embryonic fibroblast whole cell lysate, but we have failed to detect a band at 83kDa (expected size from data from other antibodies). We would appreciate any customer feedback for this antibody.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- FunctionTranscriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation.
- Sequence similaritiesBelongs to the EGR C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers.
- Cellular localizationNucleus.
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Anti-Egr1 antibody images
Anti-Egr1 antibody (ab6054) at 1/1500 dilution + Mouse embryonic fibroblast whole cell lysate at 35 µg
Goat-anti-Rabbit IgG [H&L] at 1/10000 dilution
Observed band size : 58 kDa (why is the actual band size different from the predicted?)
ab6054 at 10 µg/ml staining human ovarian cancinoma.