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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Anti-Egr1 antibody [mAbcam55160] (ab55160)
Product partner
Related products
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Proteins and Peptides
ab56196
- Secondary antibodies
- Isotype controls
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab55160? Please let us know so that we can cite the reference in this datasheet
ab55160 has been referenced in 1 publications.
- Jafferji I et al. Inhibition of epidermal growth factor receptor (EGFR) expression by human cytomegalovirus correlates with an increase in the expression and binding of Wilms' Tumour 1 protein to the EGFR promoter. J Gen Virol 90:1569-74 (2009). PubMed: 19321755
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Western blot - Egr1 antibody [mAbcam55160] (ab55160)](/ps/datasheet/Images/55/ab55160/ab55160_2.jpg)
Western blot - Egr1 antibody [mAbcam55160] (ab55160)
All lanes : Anti-Egr1 antibody [mAbcam55160] (ab55160) at 5 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 58 kDa
Observed band size : 55,85 kDa (why is the actual band size different from the predicted?)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Egr1 antibody [mAbcam55160] (ab55160)](/ps/datasheet/images/55/ab55160/Egr1-Primary-antibodies-ab55160-6.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Egr1 antibody [mAbcam55160] (ab55160)
ab55160 (1/250) staining Egr1 in paraffin-embedded Human placenta tissue. Tissue underwent fixation in formaldehyde, peroxidase blocking, protein blocking and heat mediated antigen retrieval. The secondary antibody was goat anti rabbit/mouse conjugated to HRP. For further experimental details please refer to abreview.
This image is courtesy of an abreview submitted by Antibody Solutions Ltd.
](/ps/datasheet/images/55/ab55160/Egr1-Primary-antibodies-ab55160-10.jpg)
Flow Cytometry-Anti-Egr1 antibody [mAbcam55160](ab55160)
Overlay histogram showing HeLa cells stained with ab55160 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55160, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (
![Egr1 antibody [mAbcam55160] for IHC-P in Human (55160)](/ps/datasheet/images/55/ab55160/Egr1-Primary-antibodies-ab55160-2.jpg)
Egr1 antibody [mAbcam55160] for IHC-P in Human (55160)
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