![Western blot - Egr1 antibody [mAbcam55160] (ab55160) image](#ab55160_2.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Egr1 antibody [mAbcam55160] (ab55160) image](#Egr1-Primary-antibodies-ab55160-6.jpg)
Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Overview
Product name
Anti-Egr1 antibody [mAbcam55160]
See all Egr1 products (4) ...
Description
Mouse monoclonal [mAbcam55160] to Egr1
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human Egr1.
(Peptide available as ab56196.)
Positive control
This antibody gave a positive signal in the following lysates: HeLa Whole Cell Lysate; HeLa Nuclear lysate - TPA stimulated 2 hr
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
mAbcam55160
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Applications
Show applications key
Our Abpromise guarantee covers the use of ab55160 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
IHC-P: 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use at a concentration of 5 µg/ml. Detects a band of approximately 55, 85 kDa (predicted molecular weight: 58 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation.
Sequence similarities
Belongs to the EGR C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP18146
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AT 225 antibody
- AT225 antibody
- Early growth response 1 antibody
see all
Database links
- Entrez Gene: 1958 Human
- Entrez Gene: 13653 Mouse
- Entrez Gene: 24330 Rat
- Omim: 128990 Human
- SwissProt: P18146 Human
- SwissProt: P08046 Mouse
- SwissProt: P08154 Rat
- Unigene: 326035 Human
see all
Anti-Egr1 antibody [mAbcam55160] images:
Western blot - Egr1 antibody [mAbcam55160] (ab55160)
![Western blot - Egr1 antibody [mAbcam55160] (ab55160)](/ps/datasheet/Images/55/ab55160/ab55160_2.jpg)
All lanes : Anti-Egr1 antibody [mAbcam55160] (ab55160) at 5 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate - TPA stimulated 2 hr (ab14653)
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 58 kDa
Observed band size : 55,85 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Egr1 antibody [mAbcam55160] (ab55160)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Egr1 antibody [mAbcam55160] (ab55160)](/ps/datasheet/images/55/ab55160/Egr1-Primary-antibodies-ab55160-6.jpg)
ab55160 (1/250) staining Egr1 in paraffin-embedded Human placenta tissue. Tissue underwent fixation in formaldehyde, peroxidase blocking, protein blocking and heat mediated antigen retrieval. The secondary antibody was goat anti rabbit/mouse conjugated to HRP. For further experimental details please refer to abreview.
This image is courtesy of an abreview submitted by Antibody Solutions Ltd.
References for Anti-Egr1 antibody [mAbcam55160] (ab55160)
This product has been referenced in:
- Jafferji Iet al. Inhibition of epidermal growth factor receptor (EGFR) expression by human cytomegalovirus correlates with an increase in the expression and binding of Wilms' Tumour 1 protein to the EGFR promoter. J Gen Virol 90:1569-74 (2009).Read more (PubMed: 19321755) »
See 1 publication for this product
Publishing research using ab55160? Please let us know so that we can cite the reference in this datasheet
![Egr1 antibody [mAbcam55160] for IHC-P in Human (55160)](/ps/datasheet/images/55/ab55160/Egr1-Primary-antibodies-ab55160-2.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"