Overview

  • Product nameAnti-Egr1 antibody [mAbcam55160]See all Egr1 primary antibodies ...
  • Description
    Mouse monoclonal [mAbcam55160] to Egr1
  • Tested applicationsWB, IP, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human Egr1.

    (Peptide available as ab56196.)

  • Positive control
    • This antibody gave a positive signal in the following lysates: HeLa Whole Cell Lysate; HeLa Nuclear lysate - TPA stimulated 2 hr
  • General notesWe can conjugate this antibody to FITC for you (please see ab150245 for details).

Properties

Applications

Our Abpromise guarantee covers the use of ab55160 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 µg/ml. Detects a band of approximately 55, 85 kDa (predicted molecular weight: 58 kDa).Can be blocked with Human Egr1 peptide (ab56196).
IP Use a concentration of 5 µg/ml.
IHC-P 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt Use 1µg for 106 cells.

Target

  • FunctionTranscriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation.
  • Sequence similaritiesBelongs to the EGR C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AT 225 antibody
    • AT225 antibody
    • Early growth response 1 antibody
    • Early growth response protein 1 antibody
    • EGR 1 antibody
    • EGR-1 antibody
    • EGR1 antibody
    • EGR1_HUMAN antibody
    • G0S 30 antibody
    • G0S30 antibody
    • KROX 24 antibody
    • Krox 24 protein antibody
    • KROX24 antibody
    • Nerve growth factor induced protein A antibody
    • Nerve growth factor-induced clone A antibody
    • Nerve growth factor-induced protein A antibody
    • NGFI A antibody
    • NGFI-A antibody
    • NGFIA antibody
    • TIS 8 antibody
    • TIS8 antibody
    • Transcription factor ETR 103 antibody
    • Transcription factor ETR103 antibody
    • Transcription factor Zif 268 antibody
    • Transcription factor Zif268 antibody
    • Zfp 6 antibody
    • ZIF 268 antibody
    • ZIF268 antibody
    • Zinc finger protein 225 antibody
    • Zinc finger protein Krox-24 antibody
    • ZNF 225 antibody
    • ZNF225 antibody
    see all

Anti-Egr1 antibody [mAbcam55160] images

  • Egr1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Egr1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab55160.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 55kDa; Egr1

  • All lanes : Anti-Egr1 antibody [mAbcam55160] (ab55160) at 5 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate - TPA stimulated 2 hr (ab14653)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 58 kDa
    Observed band size : 55,85 kDa (why is the actual band size different from the predicted?)
  • ab55160 (1/250) staining Egr1 in paraffin-embedded Human placenta tissue.  Tissue underwent fixation in formaldehyde, peroxidase blocking, protein blocking and heat mediated antigen retrieval.  The secondary antibody was goat anti rabbit/mouse conjugated to HRP.  For further experimental details please refer to abreview.

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab55160 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55160, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-Egr1 antibody [mAbcam55160] (ab55160)

This product has been referenced in:
  • Jafferji I  et al. Inhibition of epidermal growth factor receptor (EGFR) expression by human cytomegalovirus correlates with an increase in the expression and binding of Wilms' Tumour 1 protein to the EGFR promoter. J Gen Virol 90:1569-74 (2009). Read more (PubMed: 19321755) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Placenta)
Specification Placenta
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval Solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
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Verified customer

Submitted Mar 25 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"